Combination of baicalein and temozolomide eradicates malignant glioma through cell apoptosis

Author(s): Lingling DING, Linlin YUE, Linlin Wang, Yingying Li, Ling LI, Xiangmin YU

To study the synergistic effect of baicalein (BAI) combined with temozolomide (TMZ) on glioma cells,weselectedU251 cells as experimental cell. CCK-8 assay was used to detect the effect of baicalein、temozolomide and baicalein combined with temozolomide on the viability of U251 cells. Western blot was detected the expression of cleaved caspase-3 and Bcl-2 protein. DAPI staining was used to observe the changes of nuclei during apoptosis. The results show that the combination of baicalein and temozolomide was more effective than temozolomide and baicalein alone (p <0.000), and the expression of cleaved caspase-3 and Bcl-2 protein was significantly increased in U251 cells treated with temozolomide and baicalein. DAPI staining showed that the nucleus disintegration was very obvious when the drugs were combined.

glioma cells:temozolomide, baicalein, apoptosis

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