Effects of Combination Therapy with Cisplatin and Grape Seed Proanthocyanidins on Human Cervical Cancer Cells C33A

Effects of Combination Therapy with Cisplatin and Grape Seed Proanthocyanidins on Human Cervical Cancer Cells C33A

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Author(s)

Author(s): Jing Wang, Hong Yu

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DOI: 10.18483/ijSci.973 434 701 101-106 Volume 5 - Mar 2016

Abstract

To investigated the inhibitory effect of cisplatin in combination with Grape Seed Proanthocyanidins against human cervical cancer cell lines in vitro. Cervical cancer C33A cells were cultured and divided into four groups: cisplatin group, Grape Seed Proanthocyanidins group, cisplatin with Grape Seed Proanthocyanidins group and the blank control group. Morphologic changes of C33A cells were observed under invert microscope after C33A cells were treated 48, and 72 h. The proliferation of C33A cells was determined by CCK8 assay. Apoptosis were evaluated by PE Annexin V staining. Both of these two agents induced apoptosis and inhibited proliferation in C33A cells in a dose-dependent manner. In combination, cisplatin and Grape Seed Proanthocyanidins amplified these regulatory effects compared to each agent alone. The inhibition of proliferation can be observed in cisplatin group and in Grape Seed Proanthocyanidins group with different concentrations, while the notable effect was showed in Grape Seed Proanthocyanidins group. Combination treatment of cisplatin and Grape Seed Proanthocyanidins produces synergistic influence on C33A cells.

Keywords

cisplatin, Grape Seed Proanthocyanidins, human cervical cancer cells C33A

References

  1. Granados López A J, López J A. Multistep model of cervical cancer: Participation of miRNAs and coding genes[J]. International journal of molecular sciences, 2014;15(9): 15700-15733.
  2. Greer B E, Koh W J, Abu-Rustum N R, et al. Cervical cancer[J]. Journal of the National Comprehensive Cancer Network, 2010; 8(12): 1388-1416.
  3. Cepeda V, Fuertes M A, Castilla J, et al. Biochemical mechanisms of cisplatin cytotoxicity[J]. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 2007;7(1): 3-18.
  4. Lundstrom K, Boulikas T. Viral and non-viral vectors in gene therapy: technology development and clinical trials[J]. Technology in cancer research & treatment, 2003; 2(5): 471-485.
  5. Hogan S, Lei Z, Li J, et al. Antioxidant rich grape pomace extract suppresses postprandial hyperglycemia in diabetic mice by specifically inhibiting alpha-glucosidase.[J]. Nutrition & Metabolism, 2010;7(5):1-9
  6. Sharma S D, Meeran S M, Katiyar S K. Proanthocyanidins Inhibit In vitro and In vivo Growth of Human Non–Small Cell Lung Cancer Cells by Inhibiting the Prostaglandin E2 and Prostaglandin E2 Receptors[J]. Molecular cancer therapeutics, 2010; 9(3): 569-580.
  7. Hsiu-hui Chen, and jun-ying qu., cisplatin, different concentrations of cervical cancer Hela proliferation. Chinese journal of clinical pharmacology ,2014;30(7) : 601-602.
  8. Jemal, Ahmedin, et al. Global cancer statistics.CA: a cancer journal for clinicians, 2011;61(2): 69-90.
  9. Rose P G. Chemoradiotherapy for cervical cancer[J]. European Journal of Cancer, 2002;38(2): 270-278.
  10. Chen, Qing, Xiao-Fang Liu, and Peng-Sheng Zheng. ,Grape seed proanthocyanidins (GSPs) inhibit the growth of cervical cancer by inducing apoptosis mediated by the mitochondrial pathway. 2014;9(9): 1-12.
  11. hui zhen, et al. Grape seed procyanidins to people caused by cisplatin embryo antagonism between renal cell toxicity. Food science, 2011;32(7) : 315-318.

Cite this Article:

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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