Effects Of Folic Acid And Magnesium Co-Administration 0n The Activity Of Hepatic Glucokinase (Gck) In Streptozotocin - Induced Type I Diabetic Wistar Rats

Author(s): A.D.T. Goji, A.Mohammed, Y.Tanko, Y. and M.U. Kawu

Hyperglycemia is a common feature of diabetes mellitus. It results from a decrease in glucose utilization by the liver and peripheral tissues and an increase in hepatic glucose production. Glucose phosphorylation by glucokinase is an initial event in glucose metabolism by the liver. However, glucokinase gene expression is very low in diabetic animals. Hepatic GCK is a key enzyme in glucose homeostasis and, as such, is a potential target for treatment strategies of diabetes. The present day study investigated the effect of co- administration of folic acid and magnesium on GCK activity. Thirty wistar male rats were divided into six groups of five each. Diabetic groups received 20 and 500 mg/kg folic acid and /or magnesium chloride, separarately or in combination. Diabetic control and normal control received 0.9% saline for 4 weeks. It was found that during combined exposure of folic acid and magnesium, the adverse effects of the diabetes induced by STZ was less pronounced in the group that had FA+ Mg than their individual effects. This suggests the synergistic beneficial effects of folic acid and Magnesium against STZ-induced diabetes in Wistar Rats. Investigations of the hepatic glucokinase concentration by Real-Time PCR showed a decreased in GCK concentration in diabetic control rats. GCK activity increased significantly (p<0.05) in group treated with FA+Mg. These results indicated that FA+Mg co- administration may probably exhibit a significant potential as a hypoglycemic agent perhaps via its ability to enhance GCK gene expression and its activity.

Glucokinse, folic acid, Magnesium, Diabetes Mellitus

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