Soon we are going to change Domain of ijSciences Journal along with a new look.

Effects of Selected Wound Dressings and Remedies on Planktonic Bacteria and Bacterial Biofilms of Staphylococcus aureus and Pseudomonas aeruginosa

Effects of Selected Wound Dressings and Remedies on Planktonic Bacteria and Bacterial Biofilms of Staphylococcus aureus and Pseudomonas aeruginosa


AL Quthami Khalid, Mamdouh A. Bukhari, Milton Wainwright, Soliman Abdelrazek

Download Full PDF DOI: 10.18483/ijSci.837 Downloads: 160 Views: 550 Pages: 8-16

Volume 4 - September 2015 (09)


The overall aim of this research is to evaluate the effect of some approaches of treating wounds and the use of wound dressings of on the planktonic bacteria and bacterial biofilms of Staphylococcus aureus and Pseudomonas aeruginosa, two major bacterial species found in wounds. The wound treatments were dressings containing either honey or silver, and commercial hand gels with alcohol. Results indicate that dressings that contain Manuka honey were efficacious against planktonic bacteria of S. aureus, whereas, dressings with silver were most effective against planktonic Pseudomonas aeruginosa. Panaderm (antibiotic) showed the strongest activity against both planktonic S. aureus and P. aeruginosa. The effect of wound treatments on non-established and established bacterial biofilms after 24 and 48 hours after application, show that dressings were effective against the biofilm of S. aureus. However, a non-adherent dressing with Activon Manuka Honey was ineffective against Pseudomonas aeruginosa biofilm. Overall, except for hand gels, the wound remedies tested show remarkable activity against the biofilms of S. aureus and P. aeruginosa.


  1. Al Zahrani, S. and Baghdadi, M.(2012). Evaluation of the efficiency of non-alcoholic-hand gel sanitizers products as an antibacterial. Nature Sci.10, 15-20.
  2. Benbow, M.(2005). Evidence-based wound management, London, Whurr.
  3. Bjarnsholt, T., Kirketerp-Moller, K., Jensen, P. O., Madsen, K. G., Phipps, R., Krogfelt, K., Hoiby, N. and Givskov, M.(2008). Why chronic wounds will not heal: a novel hypothesis. Wound Rep Regen, 16, 2-10.
  4. Bjarnsholt, T., Kirketerp-Moller, K., Kristiansen, S., Phipps, R., Nielsen, A. K., Jensen, P. O.,Hoiby, N. and Givskov, M.(2007). Silver against Pseudomonas aeruginosa biofilms. APMIS, 115, 921-8.
  5. Blaser, G., Santos, K., Bode, U., Vetter, H. and Simon, A. (2007a). Effect of medical honey on wounds colonised or infected with MRSA. J Wound Care, 16, 325- 8.
  6. Chaw, K. C., Manimaran, M. and Tay, F. E. (2005). Role of silver ions in destabilization of intermolecular adhesion forces measured by atomic force microscopy in Staphylococcus epidermidis biofilms. Antimicrob Agents Chemother, 49, 4853-9.
  7. Cookson, B.(2005). Clinical significance of emergence of bacterial antimicrobial resistance in the hospital environment. J Appl Microbiol, 99, 989-96.
  8. Davey, M. E. and O'Toole G. A.(2000). Microbial biofilms: from ecology to molecular genetics. Microbiol Mol Biol Rev, 64, 847-67.
  9. Davies, D. (2003). Understanding biofilm resistance to antibacterial agents. Nat Rev Drug Discov, 2, 114-22.
  10. Davies, J. (1994). Inactivation of antibiotics and the dissemination of resistance genes. Science, 264, 375-82.
  11. Davies, S. C., Ricotti, C., Cazzaniga, A., Welsh, E., Eaglstein, W. H. and Mertz, P. M.(2008). Microscopic and physiologic evidence for biofilm-associated wound colonization in vivo. Wound Rep Regen, 16, 23-9.
  12. Dunford, C. E. and Hanano, R.(2004). Acceptability to patients of a honey dressing for non-healing venous leg ulcers. J Wound Care, 13, 193-7.
  13. Escriche, I., Kadar, M., Juan-Borras, M. and Domenech, E.(2013). Suitability of antioxidant capacity, flavonoids and phenolic acids for floral authentication of honey. Impact of industrial thermal treatment. Food Chem, 142, 135-43.
  14. Gethin, G.and Cowman, S.and Conroy, R. M.(2008). The impact of Manuka honey dressings on the surface pH of chronic wounds. Int Wound J, 5, 185-94.
  15. Gupta, S., Singh, H., Varshney, A. and Prakash, P. (1992). Therapeutic efficacy of honey in infected in buffaloes. Ind. J.f An. Sci.62, 521-3.
  16. Heggers, J., Goodheart, R. E., Washington, J., Mccoy, L., Carino, E., Dang, T., Edgar, P., Maness, C. and Chinkes, D.(2005). Therapeutic efficacy of three silver dressings in an infected animal model. J Burn Care Rehabil, 26, 53-6.
  17. Hill, K. E., Malic, S., Mckee, R., Rennison, T., Harding, K. G., Williams, D. W. and Thomas, D. W.(2010). An in vitro model of chronic wound biofilms to test wound dressings and assess antimicrobial susceptibilities. J Antimicrob Chemother, 65, 1195-206.
  18. James, G. A., Swogger, E., Wolcott, R., Pulcini, E., Secor, P., Sestrich, J., Costerton, J. W. and Stewart, P. S.( 2008). Biofilms in chronic wounds. Wound Rep Regen, 16, 37-44.
  19. Klueh, U., Wagner, V., Kelly, S., Johnson, A. and Bryers, J. D.(2000). Efficacy of silver-coated fabric to prevent bacterial colonization and subsequent device-based biofilm formation. J Biomed Mater Res, 53, 621-31.;2-q
  20. Kostenko, V., Lyczak, J., Turner, K., Martinuzzi, R. J. (2010). Impact of Silver-containing wound dressings on bacterial biofilm viability and susceptibility to antibiotics during prolonged treatment. Antimicrob Agents Chemother. 54(12): 5120-31.
  21. Kwakman, P. H. and Zaat, S. A.(2012). Antibacterial components of honey. IUBMB Life, 64, 48-55.
  22. Kwakman, P. H., Te Velde, A. A., De Boer, L., Speijer, D., Vandenbroucke- Grauls, C. M. and Zaat, S. A.(2010). How honey kills bacteria. FASEB J, 24, 2576- 82.
  23. Lansdown, A. B. (2002). Silver. I: Its antibacterial properties and mechanism of action. J Wound Care, 11, 125-30.
  24. Marchetti, M. G., Kampf, G., Finzi, G. and Salvatorelli, G.(2003). Evaluation of the bactericidal effect of five products for surgical hand disinfection according to prEN 12054 and prEN 12791. J Hosp Infect, 54, 63-7.
  25. Merritt, J. H., Kadouri, D. E. and O'Toole, G. A. (2005). Growing and analyzing static biofilms. Curr Protoc Microbiol, Chapter 1, Unit 1B1.
  26. Moore, O. A., Smith, L. A., Campbell, F., Seers, K., Mcquay, H. J. and Moore, R. A. (2001). Systematic review of the use of honey as a wound dressing. BMC Complement Altern Med, 1, 2.
  27. Percival, S. L., Bowler, P. and Woods, E. J. (2008). Assessing the effect of an antimicrobial wound dressing on biofilms. Wound Rep Regen, 16, 52-7.
  28. Percival, S. L., Bowler, P.G. and Dolman, J.(2007). Antimicrobial activity of silver- containing dressings on wound microorganisms using an in vitro biofilm model. Int Wound J, 4, 186-91.
  29. Pietsch, H. (2001). Hand antiseptics: rubs versus scrubs, alcoholic solutions versus alcoholic gels. J Hosp Infect, 48, S33–S36
  30. Sharp, A. (2009). Beneficial effects of honey dressings in wound management. Nurs Stand, 24, 66-8, 70, 72 passim.
  31. Sternberg, C., Christensen, B. B., Johansen, T., Toftgaard Nielsen, A., Andersen, J. B., Givskov, M. and Molin, S.(1999). Distribution of bacterial growth activity in flow-chamber biofilms. Appl Environ Microbiol, 65, 4108-17.
  32. Topham, J.(2002). Why do some cavity wounds treated with honey or sugar paste heal without scarring? J Wound Care, 11, 53-5.
  33. Wirtanen, G., Salo, S., Helander, I. M. and Mattila-Sandholm, T. (2001). Microbiological methods for testing disinfectant efficiency on Pseudomonas biofilm. Colloid Surf B Biointerfaces, 20, 37-50.

Cite this Article:

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Issue March 2017

Volume 6, March 2017

Table of Contents

Order Print Copy

World-wide Delivery is FREE

Share this Issue with Friends:

Submit your Paper