Author(s): Rodríguez N, Rivera A., Fernández C, Mondeja B, Alvarez A
Among premature infants, the bacterial colonization of the respiratory tract has been associated with the development of pneumonia, chronic lung disease, acute respiratory insufficiency, and even death. Genital mycoplasmas, particularly Mycoplasma hominis, M. genitalium, and Ureaplasma spp., have been associated with these pathological conditions, and also with neonatal pneumonia and respiratory dystress syndrome. The objective of this study was to determine the frequency of M. hominis, M. genitalium, U. parvum and U. urealyticum in newborn child’s, and to determine whether its presence is associated with the development of respiratory complications in these patients. Were analyzed 62 respiratory secretion samples (27 nasal swabs and 35 endotracheal tubes) obtained from 34 mechanical ventilated infants. Was realized the bacteriological culture for the isolation of Ureaplasma and Mycoplasma spp., and the PCR for the Mollicutes Class and Multiplex-PCR for the identification of U. urealyticum, U. parvum, M. hominis and M. genitalium species. The bacteriological culture was positive in one sample to mycoplasmas, while with the PCR for the Mollicutes Class was obtained 25 positive samples. From these, with the Multiplex-PCR were identified three positive samples to U. parvum, two positive to U. urealyticum and one positive to M. hominis. These six samples showed coinfection with other bacteria. Were identified mycoplasmas and ureaplasma species in new born ventilated child´s. Statistical association was not demonstrated between the mycoplasmas or ureaplasmas infection and respiratory complications in the infant.
Urogenital mycoplasmas, new born child, respiratory complications
- Lahra MM, Beeby PJ, Jeffery HE. Intrauterine inflammation, neonatal sepsis, and chronic lung disease: a 13-year hospital cohort study. Pediatrics 2009; 123(5):1314-1319.
- Kataoka S, Yamada T, Chou K, Nishida R, Morikawa M, Minami M, Yamada H, Sakuragi N, Minakami H. Association between preterm birth and vaginal colonization by mycoplasmas in early pregnancy. J. Clin. Microbiol. 2006; 44(1):51-55.
- Viscardi RM, Othman AA, Hassan HE, Eddington ND, Abebe E, Terrin ML, Kaufman DA, Waites KB. Azithromycin to prevent bronchopulmonary dysplasia in ureaplasma-infected preterm infants: pharmacokinetics, safety, microbial response, and clinical outcomes with a 20-milligram-per-kilogram single intravenous dose. Antimicrob. Agents Chemother. 2013; 57(5):2127-2133.
- Glaser K, Speer CP. Neonatal CNS infection and inflammation caused by Ureaplasma species: rare or relevant? Expert. Rev. Anti. Infect. Ther. 2015; 13(2): 233-248.
- Sung TJ, Xiao L, Duffy L, Waites KB, Chesko KL, Viscardi RM. Frequency of Ureaplasma serovars in respiratory secretions of preterm infants at risk for bronchopulmonary dysplasia. Pediatr. Infect. Dis. J. 2011; 30(5): 379-383.
- Payne MS, Goss KC, Connett GK, Legg JP, Bruce KD, Chalkerd V. A quantitative analysis of Ureaplasma urealyticum and Ureaplasma parvum compared with host immune response in preterm neonates at risk of developing bronchopulmonary dysplasia. J. Clin. Microbiol. 2011; 50(3): 909-914.
- Viscardi RM. Ureaplasma species: role in diseases of prematurity. Clin. Perinatol. 2010; 37(2): 393-409.
- Turner MA, Jacqz-Aigrain E, Kotecha S. Azithromycin, Ureaplasma and chronic lung disease of prematurity: a case study for neonatal drug development. Arch. Dis. Child. 2012; 97(6): 573-577.
- Sobouti B, Fallah S, Mobayen M, Noorbakhsh S, Ghavami Y. Colonization of Mycoplasma hominis and Ureaplasma urealyticum in pregnant women and their transmission to offspring. Iran J. Microbiol. 2014; 6(4): 219-224.
- Bölske G. Survey of mycoplasma infections in cell cultures and a comparison of detection methods. Zentralbl. Bakteriol. Microbiol. Hyg. 1988; 269(3): 331-340.
- Fernández CM, Alvarez K, Muy L, Martínez M. Detection using molecular biology techniques of Mycoplasma hominis and Ureaplasma urealyticum in urogenital samples. Rev. Argent. Microbiol. 1998; 30(2): 53-58.
- Beeton ML, Maxwell NC, Davies PL, Nuttall D, McGreal E, Chakraborty M, Spiller OB, Kotecha S. Role of pulmonary infection in the development of chronic lung disease of prematurity. Eur. Respir. J. 2011; 37(6): 1424-1430.
- Pansieri C, Pandolfini C, Elie V, Turner MA, Kotecha S, Jacqz-Aigrain E, Bonati M. Ureaplasma, bronchopulmonary dysplasia, and azithromycin in European neonatal intensive care units: a survey. Sci. Rep. 2014; 12(4): 4076.
- Mirnejad R, Amirmozafari N, Kazemi B. Simultaneous and rapid differential diagnosis of Mycoplasma genitalium and Urea¬plasma urealyticum based on a polymerase chain reaction-re¬striction fragment length polymorphism. Indian J. Med. Microbiol. 2011; 29(1):33-36.
- Sung TJ. Ureaplasma infections in pre-term infants: recent information regarding the role of Ureaplasma species as neonatal pathogens. Korean J. Pediatr. 2010; 53(12):989-993.
- Sung TJ, Xiao L, Duffy L, Waites KB, Chesko KL, Viscardi RM. Frequency of ureaplasma serovars in respiratory secretions of preterm infants at risk for bronchopulmonary dysplasia. Pediatr. Infect. Dis. J. 2011; 30(5): 379-383.
- Torres-Morquecho A, Rivera-Tapia A, González-Velazquez F, Torres J, Chávez-Munguia B, Cedillo-Ramírez, Giono-Cerezo S. Adherence and damage to epithelial cells of human lung by Ureaplasma urealyticum strains biotype 1 and 2. Afr. J. Microbiol. Res. 2010; 4(6): 480-491.
- Webster D, Windsor H, Ling C, Windsor D, Pitcher D. Chronic bronchitis in immunocompromised patients: association with a novel Mycoplasma species. Eur. J. Clin. Microbiol. Infect. 2003; 22(9): 530-534.
- Rogers GB, Stressmann FA, Walker AW, Carroll MP, Bruce KD. Lung infections in cystic fibrosis: deriving clinical insight from microbial complexity. Expert. Rev. Mol. Diagn. 2010; 10(2):187-196.
- Rogers GB, Hoffman, LR, Whiteley M, Daniels TW, Carroll MP, Bruce KD. Revealing the dynamics of polymicrobial infections: implications for antibiotic therapy. Trends Microbiol. 2010; 18(8): 357-364.
- Payne MS, Goss KC, Connett GJ, Kollamparambil T, Legg JP, Thwaites R, Ashton M, Puddy V, Peacok JL, Bruce KD. Molecular microbiological characterization of preterm neonates at risk of bronchopulmonary dysplasia. Pediatr. Res. 2010; 67(4): 412-418.
International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.