Molecular Mechanism of EGFR Signaling Pathway Mediating Proliferation and Migration of U251 Glioma Cell Line

Author(s): Linlin YUE, Ling LI, Bin WANG, Xiangmin YU, Lingling DING, Linlin Wang, Yingying Li

The EGFR, epidermal growth factor recepor, is a 170 kDa transmembrane glycoprotein and belongs to receptor tyrosine kinases family. Actual evidence shows that overexpression of EGFR is involved in 50% –70% of various diseases. However, there is limited evidence regarding the role of EGFR in the tumorigenesis of human gliomas. In the present study, its effects on U251 cell proliferation and migration were examined using CCK and scratch-wound assay. We found that AG1478 inhibit EGF-induced U251 cells proliferation and migration. Moreover, it was demonstrated that the downregulation of p-EGFR in U251 glioma cells inhibited the expression of P-Akt. Taken together, these findings suggest that the EGFR/Akt signaling pathway involved in the regulation of U251 glioma cells proliferation and migration. It’s mechanism is possible that after the EGFR/Akt signaling pathway activated, caused the high expression of downstream proteins.

glioma, epidermal growth factor, proliferation, migration

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