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Volume 6 - May 2017
Some herbal antiplasmodial plants have not been investigated for their hepatotoxic and glycogen lowering effects in experimental malaria models. We investigated the histochemical and immunohistochemical effects of ethanolic leaves extract of Nauclea latifolia (NL) an antiplasmodial plant on the liver of experimental prophylactic malaria mice. Twenty (20) mice (20-24 g) were grouped after acclimatization as follows: group A administered normal saline for 3 days, then inoculated with Plasmodium berghei (Pb); group B received 500 mg per kg body weight extract for 3 days then infected with Pb; group C received 1000 mg per weight extract for 3 days then infected with Pb; group D received 5 mg per kg body weight Artemether/lumefantrine (AL) for 3 days then infected with Pb. Extracts and drug were administered orally via oro-gavage needle. Inoculums of Pb at 1x106 were injected intraperitoneally and were subsequently monitored for 72 hrs, then fasted over night, and humanely sacrificed with liver tissues excised and processed for light microscopy. Result of routine hematoxylin and eosin stain in Figure 1 revealed that group A had the most heptocellular distortions and inflammation with prominent hyperplasia compared to extract and drug treated groups (groups B - D); in figure 2 periodic acid-Schiff (PAS) showed that group A had moderate PAS expression of glycogen stores; extract groups had depleted glycogen stores compared to group D – the AL group had more glycogen stores. In figure 3 the cytokeratin-7 showed that groups A - C had moderate positivity, while group D was mild/lower. In conclusion NL ethanolic leaf extract is moderately hepatoprotective in a dose dependent manner, but decreased periodic acid-Schiff (PAS) expression of glycogen granules and moderately up-regulated cytokeratin-7 in experimental prophylaxis malaria mice.
Hepatocytes, Malaria, Prophylaxis, Periodic acid-Schiff, Cytokeratin-7
- Ajaiyeoba E, Ashidi J, Abiodun O, Okpako L, Ogbole O, Akinboye D, Falade C, Bolaji O, Gbotosho G, Falade M, Itiola O, Houghton P, Wright Collins, and Oduola A (2004) Antimalarial Ethnobotany: In Vitro Antiplasmodial Activity of Seven Plants Identified in the Nigerian Middle Belt. Pharmaceutical Biology, 42(8):588-5912 (
- Akubue P and Mittal GC. 1982. Clinical evaluation of a traditional herbal practice in Nigeria: A preliminary report. J. Ethnopharmacol., 6:355-359.
- Cardiff RD, Miller CH, Munn RJ (2008). Manual hematoxylin and eosin staining of mouse tissue sections. Cold Spring Harb. Protoc. doi:10.1101/pdb.prot073411.
- Effiong AE, Ebong P, Eseyin OA (2013) Hypoglycemic Effect of Ethanol Extract and Fractions of Nauclea latifolium leaf on normal and alloxaninduced diabetic rats. International J of Biochemistry and Biotechnology, 2(6)457-460
- Edagha IA, Atting IA, Bassey Rb, Bassey EI, Ukpe SJ (2014). Erythropoitic and Hepatoprotective Potential of Ethanolic Extract of Nauclea latifolia in Mice Infected with Plasmodium berghei berghei, American J. of Medical Sciences and Medicine 2(1)7-12of B International Journal
- Faddis BT, Vijayan VK (1988). Application of glial fibrillary acidic protein immunohistochemistry in quantification of astrocytes in the rat brain. American Journal of Anatomy. 183:316-322.
- Farombi EO (2003). African Indigenous Plants with Chemotherapeutic potentials and Biotechnological Approach to the Production of Bioactive Prophylactic Agents. Afr J Biotechnol., 2:662-671
- Gates L, Adler RR, Elangbam CS (2016) Osmium tetroxide post-fixation and periodic acid-Schiff dual staining technique to demonstrate intracellular liupid and glycogen in the mouse liver section – a novel method for co-visualization of intracellular contents in paraffin-embedded tissue. J. of Histotechnology 39(1):2-7
- Gidado A, Danladi A, Ameh A, Sunday, Atawodi E. and Ibrahim S (2008) Hypoglycaemic Activity of Nauclea Latifolia Sm. (Rubiaceae) In Experimental Animals. Afr. J. Trad. Cam 5 (2): 201 – 208
- Igwe CU, Ojiako AO, Emejulu AA and Iwueke AV (2012). Phytochemical analyses of plants traditionally used for malaria treatment in Southeastern Nigeria. Journal of Research in Biochemistry, 1: 015-022
- Julian MT, Alonso N, Ojanguren I, Pizarro E, Ballester E, Puig-Domingo M (2015). Hepatic glycogenosis: An underdiagnosed complication of diabetes mellitus? World Journal of Diabetes 6(2):321-325
- Krishna M (2013). Role of Special Stains in Diagnostic Liver Pathology. Clinical Liver Disease 2(SI) S8-S10
- Kumar P, Clark M (2012). Kumar and Clark’s Clinical Medicine (Eight edn) Drugs and the Liver. In Liver, biliary tract and pancreatic disease. Saunders Publishers pp. 348-349
- Lopez-Panquera R (2013) Hepatopathology for Gastroenterologist and Hepatologists. Rev Col Gastroenlerol 28(2)152-159
- National Institute of Health (2011). Guide for the care and use of laboratory animals, 8th edition, Washington (DC): National Academies Press (US) pp 1-217.
- O’Callaghan J, Sairam K (2005). Glial fibrillary acidic protein and related glial proteins as biomakers of neurotoxicity. Expert Opinion on Drug Safety. 4:433-442.
- Odetola A, Basir O (1980). Evaluation of antimalarial properties of some Nigerian medicinal plants. In: Sofowora, A. (Ed.), In: Proceedings of African bioscience network, federal ministry of science and technology, Nigerian society of pharmacology and drug research and production unit, University of Ife organized workshop, Ife. 275-283.
- Olorunnisola OS, Afolayanin AJ (2011). In vivo anti-malaria activity of methanolic leaf and root extracts of Sphenocentrum jollyanum Pierre. African Journal of Pharmacy and Pharmacology. 5(14):1669-1673.
- Orah N, Rotimi O, Abdulkareem FB (2016). The Use of Special Stains in Liver Biopsy Interpretation: Implications for the management of Liver disease in Nigeria. Nigerian J. of Clinical Practice, 19(4)523-529
- Peters W (1965). Drug resistance in Plasmodium berghei. Experimental Parasitology. 17:80-89.
- Prommano O, Chaisri U, Turner GD, Wilairatana P, Ferguson DJ, Viriyavejakul P, White NJ, Pongponratn E (2005). A quantitative ultrastructural study of the liver and spleen in fatal falciparum malaria. Southeast Asian J Trop Med Public 2005, 36:1359-1370
- Rupani AB, Amarapurkar AD (2009). Hepatic changes in fatal malaria: an emerging problem. Ann Trop Medd Parasitol., 103:119-127
- Saleh HA, Abu-Rashed AH (2007). Liver Biopsy Remains the gold standard for Evaluation of Chronic Hepatitis and Fibrosis. J Gastrointetin Liver Dis 16:425-426
- Sprangers F, Thien HV, Ackermans MT, Endert E, Sauerwein HP (2004). Glycogenolysis during short-term fasting in Malaria and Healthy Subjects – the Potential regulatory role of glycogen content on glycogen breakdown: a hypothesis. Clinical Nutrition, 23 (5): 1051-1059
- Tiono AB, Tinto H, Alao MJ, Meremikwu M, Tshefu A, Ogutu B, Ouedraogo A, Lingani M, Cousin M, Lefèvre G, Jain JP, Duparc S and Hamed K (2015). Increased systemic exposures of artemether and dihydroartemisinin in infants under 5 kg with uncomplicated Plasmodium falciparum malaria treated with artemether-lumefantrine (Coartem®). Malaria Journal, 14:157
- Whitten R, Milner DA Jr, Jeh MM, Kamiza S, Molyneux ME, Taylor TE (2011). Liver pathology in Malawian Children with Fatal Encephalopathy. Hum Pathol., 42:1230-1239
- World Health Organization (WHO) (2010). Guidelines for the treatment of malaria, Second Edition pp 1- 194.
- World Health Organization (2012). World Malaria Report, Geneva, pp. 1 - 2.
- World Health Organization (2016). World Malaria Report, Geneva, p 18
- Yarpuzlu B, Ayyildiz M, Tok OE, Aktas RG, Basdogan C (2014). Correlation between the Mechanical and Histological Properties of Liver Tissue. J of the Mechanical Behaviour of Biological Materials 403-416. Doi.org/10.1016/j.jmbbm.2013.09.016
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