Extract Ethyl Acetate Red Fruit (Pandanus Conoideus Lam.) as a Counterstain in Gram Staining Technique

Extract Ethyl Acetate Red Fruit (Pandanus Conoideus Lam.) as a Counterstain in Gram Staining Technique

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Author(s)

Author(s): Warta Dewi, Indrati, Inne Suherna Sasmita

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DOI: 10.18483/ijSci.1357 153 557 28-32 Volume 6 - Jul 2017

Abstract

Objective: to test the extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.) As dye substitution in Gram staining technique. Material and Methods: Made Preparations from the suspension Escherichia coli ATCC 25922 and Streptococcus sanguine ATCC 10556 counted 30 preparation, and then done Gram staining process to this preparation. 1 preparation using counterstain basic fuchsin as a control and 29 other preparations using ethyl acetate extract of Red Fruit as a counterstain, the result of staining compared to using standard degradation of RHS color chart. Result: From 29 sample preparations used the counterstain extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.), 12 preparations produced a color close to color code 62B on the standard degradation of RHS color chart that is moderate purplish pink. Conclusions: From this preliminary study that examined the extract of ethyl acetate Red Fruit as a counterstain on Gram staining resulted in a color contrast between Gram + and Gram -bacteria though not as good as if using compressed/counterstain basic fuchsin.

Keywords

Red fruit (Pandanus Conoidenus Lam.), Counterstain, Gram Staining Technique

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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