Objective: to test the extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.) As dye substitution in Gram staining technique. Material and Methods: Made Preparations from the suspension Escherichia coli ATCC 25922 and Streptococcus sanguine ATCC 10556 counted 30 preparation, and then done Gram staining process to this preparation. 1 preparation using counterstain basic fuchsin as a control and 29 other preparations using ethyl acetate extract of Red Fruit as a counterstain, the result of staining compared to using standard degradation of RHS color chart. Result: From 29 sample preparations used the counterstain extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.), 12 preparations produced a color close to color code 62B on the standard degradation of RHS color chart that is moderate purplish pink. Conclusions: From this preliminary study that examined the extract of ethyl acetate Red Fruit as a counterstain on Gram staining resulted in a color contrast between Gram + and Gram -bacteria though not as good as if using compressed/counterstain basic fuchsin.
Red fruit (Pandanus Conoidenus Lam.), Counterstain, Gram Staining Technique
- Capuccino, JG & Sherman, N. 2008. Microbiology; a laboratory manual, 8th ed person- Benjamin Cumming; Pp15-19.
- Johnson, RT & Case, CL. 2007. Laboratory experiment in microbiology publishing person Education Inc, San Francisco CA; Pp 3, 25,35
- Priyono SH. 2008. Consideration of red fruit conservation through plant tissue culture, extraction, fractionation of antioxidant test and anti-diabetic test. Journal of Environmental Technology. 9 (3), 227-234.
- Budi IM, Paimin FR. 2005. Red Fruit. The Swadaya spreader. Depok. Page 12-17, 20-26, 43-48.
- Samaranayake L, L. 2006. Essential Microbiology for Dentistry. 3th ed Churchill Livingstone; L Sevier. pp 7-11, 103-142.
- http://www.google.com/images , your color royal society horticultural 2010.
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