Extract Ethyl Acetate Red Fruit (Pandanus Conoideus Lam.) as a Counterstain in Gram Staining Technique

Extract Ethyl Acetate Red Fruit (Pandanus Conoideus Lam.) as a Counterstain in Gram Staining Technique

Loading document ...
Loading page ...


Author(s): Warta Dewi, Indrati, Inne Suherna Sasmita

Download Full PDF Read Complete Article

DOI: 10.18483/ijSci.1357 173 632 28-32 Volume 6 - Jul 2017


Objective: to test the extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.) As dye substitution in Gram staining technique. Material and Methods: Made Preparations from the suspension Escherichia coli ATCC 25922 and Streptococcus sanguine ATCC 10556 counted 30 preparation, and then done Gram staining process to this preparation. 1 preparation using counterstain basic fuchsin as a control and 29 other preparations using ethyl acetate extract of Red Fruit as a counterstain, the result of staining compared to using standard degradation of RHS color chart. Result: From 29 sample preparations used the counterstain extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.), 12 preparations produced a color close to color code 62B on the standard degradation of RHS color chart that is moderate purplish pink. Conclusions: From this preliminary study that examined the extract of ethyl acetate Red Fruit as a counterstain on Gram staining resulted in a color contrast between Gram + and Gram -bacteria though not as good as if using compressed/counterstain basic fuchsin.


Red fruit (Pandanus Conoidenus Lam.), Counterstain, Gram Staining Technique


  1. Capuccino, JG & Sherman, N. 2008. Microbiology; a laboratory manual, 8th ed person- Benjamin Cumming; Pp15-19.
  2. Johnson, RT & Case, CL. 2007. Laboratory experiment in microbiology publishing person Education Inc, San Francisco CA; Pp 3, 25,35
  3. Priyono SH. 2008. Consideration of red fruit conservation through plant tissue culture, extraction, fractionation of antioxidant test and anti-diabetic test. Journal of Environmental Technology. 9 (3), 227-234.
  4. Budi IM, Paimin FR. 2005. Red Fruit. The Swadaya spreader. Depok. Page 12-17, 20-26, 43-48.
  5. Samaranayake L, L. 2006. Essential Microbiology for Dentistry. 3th ed Churchill Livingstone; L Sevier. pp 7-11, 103-142.
  6. Http://www.buahmerah.org/wp-content/uploads/2016/03/Buah-Merah-Papua-.jpg
  7. http://www.google.com/images , your color royal society horticultural 2010.

Cite this Article:

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Search Articles

Issue June 2023

Volume 12, June 2023

Table of Contents

World-wide Delivery is FREE

Share this Issue with Friends:

Submit your Paper