The Significance of Notch Signaling Pathway in the Differentiation of Rat Bone Mesenchymal Stem Cells Into Schwann Cells

The Significance of Notch Signaling Pathway in the Differentiation of Rat Bone Mesenchymal Stem Cells Into Schwann Cells

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Author(s): Xiaoqin Dou, Bei Zhang, Mengyan Wang, Xiaolu Li, Yanxin Zhang, Ruowu Shen

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DOI: 10.18483/ijSci.1582 82 484 28-35 Volume 7 - Mar 2018


Objective: To investigate the role of Notch signaling pathway in the differentiation of rat bone marrow mesenchymal stem cells (MSCs) into Schwann cells (SCs). Methods: BMSCs were isolated from high glucose medium and cultured in fetal bovine serum (FBS) and α-MEM medium. The resulting cells were treated with β-mercaptoethanol (β-ME), all-trans retinoic acid (RA), platelet-derived factor (PDGF-AA), basic fibroblast growth factor (bFGF), Forskolin, Heregulin .After induction of differentiation,cells were divided into three groups: control group, all-induced group and blocker group. Notch pathway blocker DAPT was added in the blocker group upon induction. The expression of Jagg1 ligand, Hes1 target gene, Notch1, Dll1 receptor protein and signal protein (S100, p75, GFAP) were determined by quantitative RT-qPCR. Cell proliferation was measured by CCK-8. Flow cytometry was used to detect the apoptosis of MSCs .Results: Compared with the control group, the expression of SCs signal protein (S100, p75, GFAP) was significantly increased in the all-induced group and the blocker group. Compared with theall- induced group, the Jagg1 ligand , Hes1 target gene, Notch1, Dll1 receptor protein was significantly lower; compared with the all- induced of the group, the proliferation effect of the blocker group and the apoptosis rate was significantly lower than the other two groups, the difference was statistically significant, P <0.05) . Conclusion: Inhibition of Notch signaling pathway can enhance the differentiation of MSCs into SCs. The possible mechanism is that Notch signaling pathway may promote cell proliferation and promote early apoptosis of MSCs.


Notch Pathway, SCs, MSCs, Proliferation, Apoptosis


  1. Abdallah, B. M., & Kassem, M. (2008). Human mesenchymal stem cells: from basic biology to clinical applications. Gene Therapy, 15(2), 109-116.
  2. Aldahmash, A., Zaher, W., Alnbaheen, M., & Kassem, M. (2012). Human stromal (mesenchymal) stem cells: basic biology and current clinical use for tissue regeneration. Annals of Saudi Medicine, 32(1), 68.
  3. Fior, R., & Henrique, D. (2005). A novel hes5/hes6 circuitry of negative regulation controls Notch activity during neurogenesis. Dev Biol, 281(2), 318-333. doi:10.1016/j.ydbio.2005.03.017
  4. Fortier, L. A., Nixon, A. J., Williams, J., & Cable, C. S. (1998). Isolation and chondrocytic differentiation of equine bone marrow-derived mesenchymal stem cells. Am J Vet Res, 59(9), 1182-1187.
  5. Fuwa, T. J., Hori, K., Sasamura, T., Higgs, J., Baron, M., & Matsuno, K. (2006). The first deltex null mutant indicates tissue-specific deltex-dependent Notch signaling in Drosophila. Mol Genet Genomics, 275(3), 251-263. doi:10.1007/s00438-005-0087-3
  6. Jessen, K. R., & Mirsky, R. (2008). Negative regulation of myelination: relevance for development, injury, and demyelinating disease. Glia, 56(14), 1552.
  7. Pan, D., & Rubin, G. M. (1997). Kuzbanian Controls Proteolytic Processing of Notch and Mediates Lateral Inhibition during Drosophila and Vertebrate Neurogenesis. Cell, 90(2), 271-280.
  8. Preitschopf, A., Li, K., Schorghofer, D., Kinslechner, K., Schutz, B., Thi Thanh Pham, H., . . . Mikula, M. (2014). mTORC1 is essential for early steps during Schwann cell differentiation of amniotic fluid stem cells and regulates lipogenic gene expression. PLoS One, 9(9), e107004. doi:10.1371/journal.pone.0107004
  9. Wang, J., Ren, K. Y., Wang, Y. H., Kou, Y. H., Zhang, P. X., Peng, J. P., . . . Jiang, B. G. (2015). Effect of active Notch signaling system on the early repair of rat sciatic nerve injury. Artif Cells Nanomed Biotechnol, 43(6), 383-389. doi:10.3109/21691401.2014.896372

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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