Effects of Endogenous n-3PUFA on Body Weight, Autophagy and Inflammation in Mice

Effects of Endogenous n-3PUFA on Body Weight, Autophagy and Inflammation in Mice

Loading document ...
Page
of
Loading page ...

Author(s)

Author(s): Xin Fang, Chao Song, YinLin Ge, JinYu Zhang

Download Full PDF Read Complete Article

DOI: 10.18483/ijSci.1587 73 340 64-70 Volume 7 - Mar 2018

Abstract

The fat-1 transgenic mice were used as models, to investigate the effects of n-3PUFAs on body weight, the expression of inflammation and autophagy in mice, and to explore its mechanism. The mice were divided into two groups: fat-1 transgenic mice and wild-type mice. The body weight and body length were measured and the index of body weight/body length was calculated regularly every week for 8 weeks. Cardiac blood was collected for determination of TG, CT, HDL-C, LDL-C and BG. Frozen sections of liver were stained by Oil Red O to observe the lipid droplets. The expression of autophagy proteins P62, LC3 and ATG7 in the hypothalamus were detected by western blot, and the relative quantitative analysis was performed. Real-time quantitative PCR was used to quantify the mRNA expression of TNF-α, IL-6, IL-1β, IFN-γ, MCP-1, TLR-4 and adiponectin in epididymal adipose tissue. The body weight/body length of fat-1 transgenic mice was significantly lower than that of wild-type mice(P<0.05), the levels of TG, CT, HDL-C, LDL-C and BG in the serum of fat-1 transgenic mice were significantly lower than those in wild-type mice(P<0.05). Lipid droplets in the liver of fat-1 transgenic mice were significantly less. The expression of P62 in fat-1 transgenic mice was significantly down-regulated (P <0.05), while the expression of ATG7 was significantly increased (P <0.05), and the ratio of LC3 Ⅱ / Ⅰ was significantly increased(P<0.05). The results of real-time quantitative PCR showed that the mRNA relative expression of TNF-α, IL-6, IL-1β, IFN-γ, MCP-1 and TLR-4 in epididymal fat tissue of fat-1 transgenic mice was significantly decreased, and the expression of adiponectin was increased (P < 0.05). n-3PUFAs reduce the body weight to prevent obesity may by up-regulation of hypothalamic autophagy, and down-regulation of inflammation in peripheral fat.

Keywords

Hypothalamus, n-3 PUFAs, Autophagy, Inflammation

References

  1. Williams E P, Mesidor M, Winters K, et al. Overweight and Obesity: Prevalence, Consequences, and Causes of a Growing Public Health Problem[J]. Curr Obes Rep, 2015, 4(3): 363-70.
  2. Hruby A, Manson J E, Qi L, et al. Determinants and Consequences of Obesity[J]. American Journal of Public Health, 2016, 106(9): 1656-1662.
  3. Kopelman P G. Obesity as a medical problem[J]. Nature, 2000, 404(6778): 635.
  4. Song Z, Xie W, Chen S, et al. High-fat diet increases pain behaviors in rats with or without obesity[J]. Scientific Reports, 2017, 7(1).
  5. Jang H H, Nam S Y, Kim M J, et al. Agrimonia pilosa Ledeb. aqueous extract improves impaired glucose tolerance in high-fat diet-fed rats by decreasing the inflammatory response[J]. BMC Complementary and Alternative Medicine, 2017, 17(1).
  6. Hirotani Y, Fukamachi J, Ueyama R, et al. Effects of Capsaicin Coadministered with Eicosapentaenoic Acid on Obesity-Related Dysregulation in High-Fat-Fed Mice[J]. Biol Pharm Bull, 2017, 40(9): 1581-1585.
  7. Coupe B, Ishii Y, Dietrich M O, et al. Loss of autophagy in pro-opiomelanocortin neurons perturbs axon growth and causes metabolic dysregulation[J]. Cell Metab, 2012, 15(2): 247-55.
  8. Bm S, Js F. Obesity and the regulation of energy balance[J]. Cell., 2001, 104(4): 531-543.
  9. Jaishy B, Abel E D. Lipids, lysosomes, and autophagy[J]. J Lipid Res, 2016, 57(9): 1619-35.
  10. Kaushik S, Arias E, Kwon H, et al. Loss of autophagy in hypothalamic POMC neurons impairs lipolysis[J]. EMBO Rep, 2012, 13(3): 258-65.
  11. Rubinsztein D C. Autophagy—alias self-eating—appetite and ageing[J]. EMBO reports, 2012, 13(3): 173-174.
  12. Liu W, Xie X, Liu M, et al. Serum omega-3 Polyunsaturated Fatty Acids and Potential Influence Factors in Elderly Patients with Multiple Cardiovascular Risk Factors[J]. Sci Rep, 2018, 8(1): 1102.
  13. Kang J X, Wang J, Wu L, et al. Transgenic mice: fat-1 mice convert n-6 to n-3 fatty acids[J]. Nature, 2004, 427(6974): 504.
  14. Beppu F, Li H, Yoshinaga K, et al. Dietary Starfish Oil Prevents Hepatic Steatosis and Hyperlipidemia in C57BL/6N Mice Fed High-fat Diet[J]. J Oleo Sci, 2017, 66(7): 761-769.
  15. Ghandour R A, Colson C, Giroud M, et al. Impact of dietary omega3 polyunsaturated fatty acid supplementation on brown and brite adipocyte function[J], 2018.
  16. White P J, Mitchell P L, Schwab M, et al. Transgenic omega-3 PUFA enrichment alters morphology and gene expression profile in adipose tissue of obese mice: Potential role for protectins[J]. Metabolism, 2015, 64(6): 666-76.
  17. Ottestad I, Nordvi B, Vogt G, et al. Bioavailability of n-3 fatty acids from n-3-enriched foods and fish oil with different oxidative quality in healthy human subjects: a randomised single-meal cross-over study[J]. J Nutr Sci, 2016, 5: e43.
  18. Singh R. Autophagy in the control of food intake[J]. Adipocyte, 2012, 1(2): 75-79.
  19. Ghosh A K, O'brien M, Mau T, et al. Toll-like receptor 4 (TLR4) deficient mice are protected from adipose tissue inflammation in aging[J]. Aging (Albany NY), 2017.
  20. Saitoh T, Fujita N, Jang M H, et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production[J]. Nature, 2008, 456(7219): 264-8.

Cite this Article:

  • BibTex
  • RIS
  • APA
  • Harvard
  • IEEE
  • MLA
  • Vancouver
  • Chicago

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Search Articles

Issue July 2019

Volume 8, July 2019


Table of Contents


Order Print Copy

World-wide Delivery is FREE

Share this Issue with Friends:


Submit your Paper