Flavonoids from Herba epimedii Protect against MPP+-induced Toxicity in MES23.5 Cells

Flavonoids from Herba epimedii Protect against MPP+-induced Toxicity in MES23.5 Cells

Loading document ...
Page
of
Loading page ...

Author(s)

Author(s): Na Li, Mingchun Jiang, Wenfang Chen

Download Full PDF Read Complete Article

DOI: 10.18483/ijSci.1615 206 660 1-7 Volume 7 - Apr 2018

Abstract

Flavonoids, the active components of Herba epimedii (HEP), exerts many pharmacological effects, such as improvement of neurological function and sexual dysfunction, anti-osteoporosis and treatment of cardiovascular diseases in China over the centuries. According to the Chinese Pharmacopoeia, icariin, epimedin B and baohuoside-1 are three major flavonoid compounds isolated from HEP. The present study aims to characterize the neuroprotective effects of total flavonoid fraction of HEP and its three major flavonoid compounds against 1-methyl-4-phenylpyridinium ion (MPP+)-induced neurotoxicity in MES23.5 cells. MTT assay showed that treatment with MPP+ significantly suppressed the cell viability. The effect could be respectively reversed by HEP, icariin, epimedin B and baohuoside-1 treatment in a dose-dependent manner. The present results provided the evidence that HEP and its main active compounds could protect against MPP+-induced neurotoxicity in dopaminergic MES23.5 cells.

Keywords

Herba epimedii, Icariin, Epimedin B, baohuoside-1, 1-methyl-4-phenylpyridinium ion, MES23.5 Cells

References

  1. Chan, B.C.L., H.Y. Lee, W.S. Siu, K.H. Yip, C.H. Ko, C.B.S. Lau, P.C. Leung, and H.Y.A. Lau, Suppression of mast cell activity contributes to the osteoprotective effect of an herbal formula containing Herba Epimedii, Fructus Ligustri Lucidi and Fructus Psoraleae. Journal of Pharmacy & Pharmacology, 2014. 66(3): p. 437-44.
  2. Sze, S.C., Y. Tong, T.B. Ng, C.L. Cheng, and H.P. Cheung, Herba Epimedii: anti-oxidative properties and its medical implications. Molecules, 2010. 15(11): p. 7861-7870.
  3. Sun, P., Y. Wen, Y. Xu, Y. Pei, Y. Chen, N. Shimizu, and T. Takeda, [The chemical constituents of Epimedium koreanum Nakai]. Yao Xue Xue Bao, 1998. 33(12): p. 919-22.
  4. Zeng, K.W., H. Ko, H.O. Yang, and X.M. Wang, Icariin attenuates β-amyloid-induced neurotoxicity by inhibition of tau protein hyperphosphorylation in PC12 cells. Neuropharmacology, 2010. 59(6): p. 542-550.
  5. Song, Y.X., J.Y. Miao, M. Qiang, R.Q. He, X.M. Wang, and W.W. Li, Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation. Chinese Journal of Integrative Medicine, 2016. 22(6): p. 430-437.
  6. Luo, Y., J. Nie, Q.H. Gong, Y.F. Lu, Q. Wu, and J.S. Shi, Protective effects of icariin against learning and memory deficits induced by aluminium in rats. Clinical & Experimental Pharmacology & Physiology, 2007. 34(8): p. 792-795.
  7. Chen, W.F., L. Wu, Z.R. Du, L. Chen, A.L. Xu, X.H. Chen, J.J. Teng, and M.S. Wong, Neuroprotective properties of icariin in MPTP-induced mouse model of Parkinson's disease: Involvement of PI3K/Akt and MEK/ERK signaling pathways. Phytomedicine, 2017. 25: p. 93-99.
  8. Wu, L., Z.R. Du, A.L. Xu, Z. Yan, H.H. Xiao, M.S. Wong, X.S. Yao, and W.F. Chen, Neuroprotective effects of total flavonoid fraction of the Epimedium koreanum Nakai extract on dopaminergic neurons: In vivo and in vitro. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2017. 91: p. 656.
  9. Zhang, D.W., Y. Cheng, N.L. Wang, J.C. Zhang, M.S. Yang, and X.S. Yao, Effects of total flavonoids and flavonol glycosides from Epimedium koreanum Nakai on the proliferation and differentiation of primary osteoblasts. Phytomedicine, 2008. 15(1): p. 55-61.
  10. Ge, K.L., W.F. Chen, J.X. Xie, and M.S. Wong, Ginsenoside Rg1 protects against 6-OHDA-induced toxicity in MES23.5 cells via Akt and ERK signaling pathways. Journal of Ethnopharmacology, 2010. 127(1): p. 118-123.
  11. An, S. and L. Tao, Effect of kidney-tonifying herbs on ovary function and bone mass in postmenopausal women. Chinese Journal of Osteoporosis, 2000.
  12. Wu, H., E.J. Lien, and L.L. Lien, Chemical and pharmacological investigations of Epimedium species: A survey. Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques, 2003. 60(60): p. 1.
  13. Nelson, H.D., L.L. Humphrey, P. Nygren, S.M. Teutsch, and J.D. Allan, Postmenopausal hormone replacement therapy: scientific review. Jama, 2002. 288(7): p. 872-81.
  14. Zhu, H.R., Z.Y. Wang, X.L. Zhu, X.X. Wu, E.G. Li, and Y. Xu, Icariin protects against brain injury by enhancing SIRT1-dependent PGC-1α Expression in experimental stroke. Neuropharmacology, 2010. 59(1–2): p. 70-76.
  15. He, X.L., W.Q. Zhou, M.G. Bi, and G.H. Du, Neuroprotective effects of icariin on memory impairment and neurochemical deficits in senescence-accelerated mouse prone 8 (SAMP8) mice. Brain Research, 2010. 1334(3): p. 73.
  16. Wu, B., Y. Chen, J. Huang, Y. Ning, Q. Bian, Y. Shan, W. Cai, X. Zhang, and Z. Shen, Icariin improves cognitive deficits and activates quiescent neural stem cells in aging rats. Journal of Ethnopharmacology, 2012. 142(3): p. 746-753.
  17. Zhang, L., C. Shen, J. Chu, R. Zhang, Y. Li, and L. Li, Icariin decreases the expression of APP and BACE-1 and reduces the beta-amyloid burden in an APP transgenic mouse model of Alzheimer's disease. Int J Biol Sci, 2014. 10(2): p. 181-91.
  18. Xu, A.L., M.C. Jiang, X.H. Chen, and W.F. Chen, Icariin protects against MPP(+)-induced neurotoxicity in MES23.5 cells. Sheng LI Xue Bao, 2016. 68(5): p. 585-591.

Cite this Article:

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Search Articles

Issue June 2024

Volume 13, June 2024


Table of Contents



World-wide Delivery is FREE

Share this Issue with Friends:


Submit your Paper