Regulation of Feeding and Mechanisms of Intracerebroventricular Injection of Nesfatin-1 in Obese Rats

Regulation of Feeding and Mechanisms of Intracerebroventricular Injection of Nesfatin-1 in Obese Rats

Loading document ...
Loading page ...


Author(s): Xue Ruimei, Wang Mi, Leng Hui, Sun Xiangrong, Xu Luo

Download Full PDF Read Complete Article

DOI: 10.18483/ijSci.1631 75 300 64-70 Volume 7 - Apr 2018


Objective: Aim to discuss the effects of intracerebroventricular injection of nesfatin-1 on the feeding of normal rats and obese rats to clarify the mechanism of feeding regulation. Methods: Using the BioDAQ feeding monitoring system to monitor 24h food intake, bout size, meal size, bout frequency, meal frequency, meal interval and meal duration of rats. Results: Intracerebroventricular injection of nesfatin-1 reduced the amount of 24h food intake in normal rats, food intake at fifth hour significantly reduced; After intracerebroventricular injection of nesfatin-1 in normal rats, meal size, meal duration and the percentage of time spent eating significantly reduced, meal interval and meal frequency did not change significantly, satiety ratio significantly increased; Intracerebroventricular injection of nesfatin-1 reduced the amount of 20h food intake in obese rats, food intake at third hour significantly reduced; Intracerebroventricular injection of nesfatin-1 significantly reduced bout frequency, meal frequency and eating rate in obese rats, with a significant increase in meal interval and satiety ratio. Conclusion: Nesfatin-1 can inhibit the feeding of normal rats and obese rats. The normal rats showed increased satiation, the obese rats showed increased satiety, and the nesfatin-1 can regulate the feeding of normal rats and obese rats via different pathway.


Food intake, Nesfatin-1, Obesity


  1. Oh-IS, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus[J]. Nature, 2006, 443: 709–712
  2. Ayada C, Toru Ü, Korkut Y. Nesfatin-1 and its effects on different systems[J]. Hippokratia, 2015, 19(1): 4-10
  3. Chen X, Dong J, Jiang ZY. Nesfatin-1 influences the excitability of Glucosesing neurons in the hypothalamic nuclei and inhibits the food intake[J]. Regul Pept, 2012, 177: 21–26
  4. Könczöl K, Pintér O, Ferenczi S, et al. Nesfatin-1 exerts long-term effect on food intake and body temperature[J]. Int J Obes (Lond), 2013, 36(12): 1514-1521
  5. Xia ZF, Fritze DM, Li JY, et al. Nesfatin-1 inhibits gastric acid secretion via a central vagal mechanism in rats[J]. Am J Physiol Gastrointest LiverPhysiol, 2012, 303: G570–G577
  6. Dong J, Guan HZ, Jiang ZY, et al. Nesfatin-1 influences the excitability of glucosensing neurons in the dorsal vagal complex and inhibits food intake[J]. PLoS One, 2014, 9(6): e98967
  7. Kentish SJ, Li H, Frisby CL, et al. Nesfatin-1 modulates murine gastric vagal afferent mechanosensitivity in a nutritional state dependent manner[J]. Peptides, 2017, 89: 35-41
  8. Chen X, Shu X, Cong ZK, et al. Nesfatin-1 acts on the dopaminergic reward pathway to inhibit food intake[J]. Neuropeptides, 2015, 53: 45-50
  9. Kaba S, Karaman K, Kömüroğlu U, et al. Role of circulating nesfatin-1 in the underweight children with poor appetite[J]. Eur Rev Med Pharmacol Sci, 2015, 19(24): 4703-4706
  10. Kerbel B, Unniappan S. Nesfatin-1 suppresses energy intake co-local is ghrelin in the brain and gut, and alters ghrelin, cholecystokinin and orexin mRNA expression in goldfish[J]. J Neuroendocrinol, 2012, 24:366–377
  11. Akiyama Y, Yoshimura M, Nishimura K, et al. Activation of central nesfatin-1/NucB2 after intraperitoneally administered cisplatin in rats[J]. Biochem Biophys Res Commun, 2017, 490(3): 794-799
  12. Stengel A, Goebel-Stengel M, Wang L, et al. Nesfatin-1(30-59) but not the N- and C-terminal fragments, nesfatin-1(1-29) and nesfatin-1(60-82) injected intracerebroventricularly decreases dark phase food intake by increasing inter-meal intervals in mice[J]. Peptides, 2012, 35: 143–148
  13. Ünal K, Yüksel RN, Turhan T, et al. The association of serum nesfatin-1 and ghrelin levels with metabolic syndrome in patients with schizophrenia[J]. Psychiatry Res, 2017, 261: 45-49
  14. Stengel A, Mori M, Taché Y. The role of nesfatin-1 in the regulation of food intake and body weight: recent developments and future endeavors[J]. Obes Rev, 2013, 14: 859–870
  15. AlMassadi O, Lear PV, Müller TD, et al. Review of novel aspects of the regulation of ghrelin secretion[J]. Curr Drug Metab, 2014, 15: 398–413
  16. Crujeiras AB, Carreira MC, Cabia B, et al. Leptin resistance in obesity: anepigeneticl and scape[J]. LifeSci, 2015, 140: 57–63
  17. Weibert E, Stengel A. The X/A-like cell revisited - spotlight on the peripheral effects of NUCB2/nesfatin-1 and ghrelin[J]. J Physiol Pharmacol, 2017, 68(4): 497-520
  18. Hellström PM. Satiety signals and obesity[J]. Curr Opin Gastroenterol, 2013, 29: 222–227
  19. Scharner S, Prinz P, Goebel-Stengel M. Activity-based anorexia activates nesfatin-1 immunoreactive neurons in distinct brain nuclei of female rats[J]. Brain Res, 2017, 1677: 33-46
  20. Haghshenas R, Jafari M, Ravasi A, et al. The effect of eight weeks enduran cetraining and high-fat diet on appetite-regulating hormones in rat plasma[J]. Iran J Basic Med Sci, 2014, 17: 237–243
  21. Mohan H, Ramesh N, Mortazavi S, et al. Nutrients differentially regulate nucleobindin-2/nesfatin-1 in vitro in cultured stomach ghrelinoma (MGN3-1) cells and in vivo in male mice[J]. PLoS One, 2014, 9(12): e115102
  22. Nguyen LT, Saad S, Tan Y, et al. Maternal high-fat diet induces metabolic stress response disorders in offspring hypothalamus[J]. J Mol Endocrinol, 2017, 59(1): 81-92

Cite this Article:

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Search Articles

Issue June 2024

Volume 13, June 2024

Table of Contents

World-wide Delivery is FREE

Share this Issue with Friends:

Submit your Paper