Objective: Exploring the expression of cell-free RNA in urine of normal patients and lung cancer patients, analyze its clinical value in the dignosis of lung cancer. Methods: The urine samples were collected from lung cancer patients and healthy people. Extracted the total cf-RNA from all samples. The contents of total cf-RNA in two groups were detected, and the expressions of LUNX mRNA in two groups were measured by quantitative real-time PCR. Receiver-operating characteristics (ROC) curve was established to evaluate the diagnostic value of urine cf-RNA for the differentiation between lung cancer patients and the control group. Results: Compare to the normal group (50.50±4.527ng), the urinary cf-RNA content in patients (133.8±8.615ng) was significantly increased, p<0.05. The expression of urinary lunx mRNA level from patients was raised, p<0.05. ROC curve analysis showed that, in the diagnosis, AUC value of lunx mRNA was 0.83(95% CI, 0.747-0.906), p<0.05. Conclusion: The expression of urine cf-RNA and Lunx mRNA was elevated, and can be used as a potential molecular diagnostic tool for lung cancer.
Urine, Lung Cancer, cf-RNA, Diagnosis
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