Effects of Ferric Ammonium Citrate (FAC) on Rab Family in SH-SY5Y Dopaminergic Cells

Effects of Ferric Ammonium Citrate (FAC) on Rab Family in SH-SY5Y Dopaminergic Cells

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Author(s)

Author(s): Zhenlei Zhufu

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DOI: 10.18483/ijSci.1810 56 246 30-33 Volume 7 - Oct 2018

Abstract

To examine the effects of ferric ammonium citrate (FAC) on Rab family in SH-SY5Y dopaminergic cells.Western blot was used to investigate Rab7,Rab11,Rab27a and Rab35 protein levels in SH-SY5Y dopaminegic cells with FAC for 4h and 12h.When SH-SY5Y cells were treated with 100 μmol/L or 1 mmol/L FAC for 4h and 12 h, Rab7, Rab11 and Rab27a protein levels was not significantly different from those of the control group.However, Rab35 protein was significantly up-regulated in SH-SY5Y cells with 1 mmol/L FACtreatment.Iron overloadupregulates Rab35 protein expressionin SH-SY5Y dopaminergic cells.

Keywords

Iron, SH-SY5Y Cells, Rab

References

  1. Raposo, G. and W. Stoorvogel, Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol, 2013. 200(4): p. 373-83.
  2. de Lau, L.M. and M.M. Breteler, Epidemiology of Parkinson's disease. Lancet Neurol, 2006. 5(6): p. 525-35.
  3. Kalinderi, K., S. Bostantjopoulou, and L. Fidani, The genetic background of Parkinson's disease: current progress and future prospects. Acta Neurol Scand, 2016. 134(5): p. 314-326.
  4. Tambasco, N., et al., A53T in a parkinsonian family: a clinical update of the SNCA phenotypes. J Neural Transm (Vienna), 2016. 123(11): p. 1301-1307.
  5. Castellani, R.J., et al., Sequestration of iron by Lewy bodies in Parkinson's disease. Acta Neuropathol, 2000. 100(2): p. 111-4.
  6. AlDakheel, A., L.V. Kalia, and A.E. Lang, Pathogenesis-targeted, disease-modifying therapies in Parkinson disease. Neurotherapeutics, 2014. 11(1): p. 6-23.
  7. Singh, N., et al., Brain iron homeostasis: from molecular mechanisms to clinical significance and therapeutic opportunities. Antioxid Redox Signal, 2014. 20(8): p. 1324-63.
  8. Urrutia, P., et al., Inflammation alters the expression of DMT1, FPN1 and hepcidin, and it causes iron accumulation in central nervous system cells. J Neurochem, 2013. 126(4): p. 541-9.
  9. Zecca, L., et al., Iron, brain ageing and neurodegenerative disorders. Nat Rev Neurosci, 2004. 5(11): p. 863-73.
  10. Colombo, M., G. Raposo, and C. Théry, Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol, 2014. 30(1): p. 255-289.
  11. Jan, A.T., et al., Perspective Insights of Exosomes in Neurodegenerative Diseases: A Critical Appraisal. Frontiers in Aging Neuroscience, 2017. 9: p. 317.
  12. Stenmark, H., Rab GTPases as coordinators of vesicle traffic. Nat Rev Mol Cell Biol, 2009. 10(8): p. 513-25.
  13. Savina, A., et al., Rab11 promotes docking and fusion of multivesicular bodies in a calcium-dependent manner. Traffic, 2005. 6(2): p. 131-43.
  14. Hales, C.M., J.P. Vaerman, and J.R. Goldenring, Rab11 family interacting protein 2 associates with Myosin Vb and regulates plasma membrane recycling. J Biol Chem, 2002. 277(52): p. 50415-21.
  15. Wilcke, M., et al., Rab11 regulates the compartmentalization of early endosomes required for efficient transport from early endosomes to the trans-golgi network. J Cell Biol, 2000. 151(6): p. 1207-20.
  16. Baietti, M.F., et al., Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. Nature Cell Biology, 2012. 14(7): p. 677-685.
  17. Ostrowski, M., et al., Rab27a and Rab27b control different steps of the exosome secretion pathway. Nature Cell Biology, 2010. 12(1): p. 19-30; sup pp 1-13.
  18. Hsu, C., et al., Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A–C. Journal of Cell Biology, 2010. 189(2): p. 223.
  19. Dikshit, N., et al., Intracellular Uropathogenic E. coli Exploits Host Rab35 for Iron Acquisition and Survival within Urinary Bladder Cells. PLoS Pathog, 2015. 11(8): p. e1005083.

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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