The Nesfatin-1 Pathway from Hippocampus to Ventromedial Nucleus and its Regulation on Gastric Motility in Diabetic Rat

The Nesfatin-1 Pathway from Hippocampus to Ventromedial Nucleus and its Regulation on Gastric Motility in Diabetic Rat

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Author(s)

Author(s): Baohua Jing, Qing Yang, Weidong Xin, Luo Xu

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DOI: 10.18483/ijSci.1892 20 146 20-24 Volume 8 - Feb 2019

Abstract

Objective: To investigate the effect of microinjection of Nesfatin-1 into VMH on gastric motility in diabetic rats and its regulatory mechanism, and to study whether electrical stimulation of hippocampal CA1 region can regulate this process. Methods: The diabetic rat model was established, and the hippocampal-VMH nesfatin-1 pathway was observed by retrograde gold fluorescence tracing combined with fluorescence immunohistochemistry. The effects of microinjection of nesfatin-1 by VMH and electrical stimulation of hippocampus on gastric motility were observed in vivo. Results: Nesfatin-1 inhibited gastric motility in a dose-dependent manner, and astressin-B partially blocked the inhibition of nesfatin-1 on gastric motility; Nesfatin-1 immunoreactive neurons were present in the cytoplasm of hippocampal CA1 region and Nesfatin-1 was expressed in some fluorescent gold labeled cells; electrostimulation of hippocampal CA1 region could promote gastric motility in diabetic rats, and anti-NUCB2/Nesfatin-1 antibody could promote gastric motility in diabetic rats.Enhance the effect of electrical stimulation of hippocampal CA1 region on gastric motility. Conclusion: Nesfatin-1 injection into VMH can regulate gastric motility in diabetic rats, which may be related to the CRF system, and the hippocampal CA1 region participates in the regulation of gastric motility by nesfatin-1 in VMH.

Keywords

Hi, VMH, diabetic Rats, Gastric Motility

References

  1. Cao X, Zhou X, Cao Y, et al. Expression of NUCB2/Nesfatin-1 in the taste buds of rats [J]. Endocr J, 2016, 63: 37-45.
  2. Goebel-Stengel M, Wang LX, Stengel A, et al. Localization of Nesfatin-1 neurones in the mouse brain and functional implication [J]. Brain Res, 2011, 1396C: 20-34.
  3. Atsuchi K, Asakawa A, Ushikai M, et al. Centrally administered NUCB2/Nesfatin-1 inhibits feeding behaviour and gastroduodenal motility in mice [J]. NeuroReport, 2010, 21:1008-1011.
  4. Ramesh N, Gawli K, Pasupulleti V, et al. Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease [J]. Curr Pharm Des, 2017, [Epub ahead of print].
  5. Gao S, Guo F, Sun X, et al. The Inhibitory Effects of Nesfatin-1 in Ventromedial Hypothalamus on Gastric Function and Its Regulation by Nucleus Accumbens [J]. Front Physiol. 2017, 7:634-644.
  6. Schwartz MW, Woods SC, Porte Jr D, et al. Central nervous system control of food intake [J]. Nature, 2000, 404:661-671.
  7. Xu L, Sun X, Depoortere I, et al. Effect of motilin on the discharge of rat hippocampal neurons responding to gastric distension and its potential mechanism [J]. Peptides, 2008, 29:585-592.
  8. Schwartz MW, Woods SC, Porte Jr D, et al. Central nervous system control of food intake [J]. Nature, 2000, 404:661-671.
  9. Goebel M, Stengel A, Wang L, et al Central Nesfatin-1 reduces the nocturnal food intake in mice by reducing meal size and increasing inter-meal intervals [J]. Peptides, 2011, 32:36-43.
  10. Atsuchi K, Asakawa A, Ushikai M, et al. Centrally administered Nesfatin-1 inhibits feeding behaviour and gastrodunal motility in mice [J]. Neuroreport, 2010, 21:1008-1011.
  11. Liu Y, Gold MS. Human functional magnetic resonance imaging of eating and satiety in eating disorders and obesity [J]. Psychiatr Ann, 2003, 33:127-32.
  12. Matsuda M, Liu Y, Mahankali S, et al. Altered hypothalamic response to oral glucose intake in obese humans [J]. Diabetes, 1999, 48: 1801-1806.
  13. Routh VH. Glucosensing neurons in the ventromedial hypothalamic nucleus (VMN) and hypoglycemia-associated autonomic failure (HAAF) [J]. Diabetes Metab Res Rev, 2003, 19: 348-356.
  14. Okere B, Xu L, Roubos EW, et al. Restraint stress alters the secretory activity of neurons co-expressing urocortin-1, cocaine-and amphetamine-regulated transcript peptide and Nesfatin-1 in the mouse Edinger-Westphal nucleus [J]. Brain Res, 2010, 1317C: 92-99.
  15. Zorrilla EP, Tachy, Koob GF. Nibbling at CRF receptor control of feeding and gastrocolonic motility [J]. Trends PharmacolSci, 2003, 24:421-427.
  16. Tabarin A, Diz-Chaves Y, Consoli D, et al. Role of the corticotropin-releasing factor receptor type 2 in the control of food intake in mice: a mean pattern analysis [J]. Eur J Neurosci, 2007, 26:2303-2314.
  17. Gotoh K, Masaki T, Chiba S, et al. Nesfatin-1, corticotropin releasing hormone, thyrotropin-releasing hormone, and neuronal histamine interaction in the hypothalamus to regulate feeding behavior [J]. J Neurochem, 2013, 124:90-99.
  18. Price CJ, Hoyda TD, Samson WK, et al. Nesfatin-1 influences the excitability of paraventricular nucleus neurons [J]. J Neuroendocrinol, 2008, 20:245-250.
  19. Stengel A, Goebel M, Wang L, et al. Central Nesfatin-1 reduces dark-phase food intake and gastric emptying in rats: differential role of corticotropin-releasing factor 2 receptor [J]. Endocrinology, 2009, 150:4911-4919.

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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