Rapid Detection of Aspergillus Fumigatus Growth Percentage by Microplate Reader

Rapid Detection of Aspergillus Fumigatus Growth Percentage by Microplate Reader

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Author(s)

Author(s): Lin Sun, Jianye Shao, XiaoLin Wang, Bin Wang, Wei Zhao

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DOI: 10.18483/ijSci.1929 40 170 1-4 Volume 8 - Mar 2019

Abstract

In this experiment, the OD value of different concentrations of Aspergillus fumigatus cultured for 48 hours was measured on a microplate reader using a 96-well plate. The absorbance-concentration curve was plotted according to the results, and the absorbance value was used instead of visual observation to quickly detect the growth of Aspergillus fumigatus. situation. The results showed that the OD value was between 0.05 and 0.06 when visually 50%, and 100% when the OD value was greater than 0.17. The use of the OD value can be a rapid method for observing the growth of Aspergillus fumigatus, which has the advantages of high efficiency, accuracy and high throughput compared with the visual method.

Keywords

Aspergillus fumigatus, Microplate Reader, OD, Value Growth Status

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
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