Embryos and Lateral Buds Culture of Tapeinochilos Ananassae (Hassk). K. Schum.

Embryos and Lateral Buds Culture of Tapeinochilos Ananassae (Hassk). K. Schum.

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Author(s)

Author(s): Lilia Willadino, Nise de Fátima Coutinho Souto, Cláudia Ulisses, Ana Lúcia Figueiredo Porto, Terezinha Camara, Marina Medeiros de Araújo Silva

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622 1047 19-25 Volume 2 - Jul 2013

Abstract

Zygotic embryos and lateral buds of Tapeinochilos ananassae were inoculated into full or half-strength MS medium (½ MS) containing three antioxidants: ascorbic acid, activated charcoal, and PVP at concentrations of 0.25; 3.0 and 0.5 g.L-1 respectively. The ½ MS medium supplemented with 3.0 g.L-1 activated charcoal resulted in the best embryo establishment and plant development. The lateral buds showed no significant development, an intense phenolic oxidation and high microbial contamination (mainly bacterial). Enzymatic analysis of oxidized lateral buds showed a decline in peroxidase activity and an increase in polyphenoloxidase activity.

Keywords

tropical flowers, embryo culture, peroxidase, polyphenoloxidase

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