Serotypes and Multiresistant Salmonella sp. from Chicken Eggs and Laying Hens in Burkina Faso

Serotypes and Multiresistant Salmonella sp. from Chicken Eggs and Laying Hens in Burkina Faso

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Author(s)

Author(s): Soutongnooma Caroline BOUDA, Assèta KAGAMBÈGA, Laetitia BONIFAIT, Evariste BAKO, Haoua CISSE, Hadiza Bawa IBRAHIM, Françoise Le Gall, Aïssata WEREME-N’DIAYE, Sababenejo Alfred TRAORE, Marianne CHEMALY, Gilles SALVAT, Nicolas BARRO

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DOI: 10.18483/ijSci.2231 35 152 19-25 Volume 8 - Dec 2019

Abstract

The study investigated the prevalence and antibioresistance profil of Salmonella sp. serovars isolated from eggs and poultry in stock farming. A total of 245 eggs and 98 laying hens fecal matters from different breeding sheds were collected. The samples were processed for identification and serotyping of Salmonella sp using microbiological standard methods and Kauffman-White scheme respectively. Salmonella sp isolates antibiotic susceptibility to antimicrobial agents was tested by disk diffusion method. A total of 63 Salmonella isolates were recovered with positive samples from eggs (11.8%) and from faecal matter (12.24%). The successful serotyping of 53/63 isolates revealed the presence of S. Typhimurium (11.11%), S. Kentucky (1.59%), S. Ouakam (1.59%), S. Brancaster (6.35%), S Hato (6.35%), S. Essen (3.17%), S. Cannstatt (1.59%), and S. Derby (36.51%). Ten strains (15.87%) were untypable and ten (15.87%) belong to different serogroups such F and O. All the sérotypes shown resistance to at least one antibiotic while, 41 (65.08%) were multi-resistant to Erythromycin, Streptomycin, Tetracycline, Ceftriaxon, while high sensitivity was recorded for Chloramphenicol, Ciprofloxacin, Nalidixic acid, Imipenem, Cephalexin, Sulfamethoxazole–trimethoprim and Colistin Sulfate. These results suggest that eggs from stock farming are contaminated and harbour resistant Salmonella sp. It highlights worry in antibiotics use in stock farming, the need for farm workers and consumers education about safe handling of eggs.

Keywords

Salmonella Serotypes, Antimicrobial Resistance, Eggs, Layer Hens, Burkina Faso

References

  1. Adesiyun, A., Webb, L., Musai, L., Louison, B., Joseph, G., Stewart-Johnson, A., Samlal, S., Rodrigo, S., 2014. Resistance to Antimicrobial Agents among Salmonella Isolates Recovered from Layer Farms and Eggs in the Caribbean Region. Journal of Food Protection, 77: 2153-2160.
  2. Akter, M. R., Choudhury, K. A., Rahman, M. M., Islam, M. S., 2007. Seroprevalence of salmonellosis in layer chickens with isolation, identification and antibiogram study of their causal agents. Bangladesh Journal of Veterinary Medicine, 5: 32-42.
  3. Al, S., Hizlisoy, H., Ertas Onmaz, N., Yildirim, Y., Gönülalan, Z., 2016. Occurrence and antimicrobial resistance of Salmonella enterica subsp. enterica serovars Typhimurium, Enteritidis, and Typhi isolated from chicken eggs and poultry products. The Turkish Journal of Veterinary and Animal Sciences, 40: 737-743.
  4. Bajaj, B. K., Sharma, V., Thaku, R. L., 2003. Prevalence and antibiotic resistance profiles of Salmonella spp. in poultry egg. Journal of Food Science and Technology, 40 : 682-684.
  5. Barrow, P. A., Lovell, M. A., 1991. Experimental infection of egg-laying hens with Salmonella enteritidis phage type 4. Avian Pathology, 20: 335-348.
  6. Bauer, A. W., Kirby, W. M. M., Sherries, J. C., Turck, M., 1966. Antibiotic susceptibility testing. The American Journal of Pathology, 45: 493-496.
  7. Bouda, S. C., Kagambega, A., Bonifait, L., Le Gall, F., Bawa, H. I., Bako, E., Bagre, T. S., Zongo, C., Wereme-N’Diaye, A., Traore, S. A., Chemaly, M., Salvat, G., Barro; N., 2019 Prevalence and Antimicrobial Resistance of Salmonella enterica Isolated from Chicken and Guinea Fowl in Burkina Faso. International Journal of Microbiology and Biotechnology, 4 : 64-71. doi: 10.11648/j.ijmb.20190403.12.
  8. Cardoso, M. O.; Ribeiro, A. R.; Dos Santos, L. R.; Pilotto, F.; De Moraes, H. L.S.; Salle, C. T. P., Da Silveira Rocha, S. L.; Do Nascimento, V. P., 2006. Antibiotic resistance in Salmonella enteritidis isolated from broiler Carcasses. Brazilian Journal of Microbiology, 37: 368-371.
  9. Daoust, P-Y., Busby, D. G., Ferns, L., Goltz, J., McBurney, S., Poppe, C., Whitney, H., 2000. Salmonellosis in songbirds in the Canadian Atlantic provinces during winter-summer 1997-98. The Canadian Veterinary Journal, 41: 54-59.
  10. Davies, R. H., Breslin, M., 2003. Investigation of Salmonella contamination and disinfection in farm egg-packing plants. Journal of Applied Microbiology, 94: 191-196.
  11. De Oliveira, F. A., Pasqualotto, A. P., Da Silva, W. P., Tondo, E. C., 2010. Characterization of Salmonella Enteritidis isolated from human samples. Food Research International, 45: 1000-1003.
  12. Ejeta, G., Molla, B., Alemayehu, D., Muckle A., 2004. Salmonella serotypes isolated from minced meat beef, mutton and pork in Addis Ababa, Ethiopia. Revue de Médecine Vétérinaire, 155 : 547-551.
  13. Ekundayo, E. U, Ezeake, J. C., 2011. Prevalence and Antibiotic Sensitivity Profile of Salmonella Species in Eggs from Poultry Farms in Umudike, Abia State. Journal of Animal and Veterinary Advances, 10: 206-209.
  14. El-Sharkawy, H., Tahoun, A., El-Gohary, A., El-Abasy, M., El-Khayat, F., Gillespie, T., El-Adawy, H., 2017. Epidemiological, molecular characterization and antibiotic resistance of Salmonella enterica serovars isolated from chicken farms in Egypt. Gut Pathogens, 9: 8.
  15. EUCAST. European Committee on Antimicrobial Susceptibility Testing breakpoint table for interpretation of MICs and zone diameters 2017. http://www.sfm - microbiologie. org/UserFiles/files/casfm/CASFMV1_0_MARS_2017.pdf.
  16. FAO. 2019. Le devenir de l’élevage au Burkina Faso. Défis et opportunités face aux incertitudes. Rome, 56.
  17. Feasey, N. A., G. Dougan, R. A. Kingsley, Heyderman, R. S., Gordon, M. A., 2012. Invasive non-typhoidal. Salmonella disease: an emerging and neglected tropical disease in Africa. The Lancet. 379: 2489-2499.
  18. Finstad, S., O'Bryan, C. A., Marcy, J. A., Crandall, P. G., Ricke S. C., 2012. Salmonella and broiler processing in the United States: Relationship to foodborne salmonellosis. Food Research International, 45: 789-794.
  19. Foley, S. L., Nayak, R., Hanning, I. B., Johnson, T. J., Han, J., Ricke, S. C., 2011. Population dynamics of Salmonella enterica Serotypes in commercial egg and poultry production. Applied and Environmental Microbiology, 4273-4279.
  20. Humphrey, T. J., Cruickshank, J.G., Rowe, B., 1989. Salmonella Enteritidis phage type 4 and hens’ eggs. Lancet I : 281-285.
  21. Humphrey, T. J., Whitehead, A., Gawler, A. H. L., Henley, A., Rowe, B., 1991. Numbers of Salmonella enteritidis in the contents of naturally contaminated hens' eggs. Epidemiology and Infection, 106: 489-496.
  22. Im, M. C., Jeong, S. J., Kwon, Y-K., Jeong, O-M., Kang, M-S., Lee, Y. J., 2015. Prevalence and characteristics of Salmonella spp. isolated from commercial layer farms in Korea. Poultry Science 94: 1691-1698.
  23. Kagambega, A, Lienemann, Aulu, T., L., Traore, A. S., Barro, N., Siitonen, A., Haukka, K., 2013. Prevalence and characterization of Salmonella enterica from the intestines of cattle, poultry, swine and hedgehogs in Burkina Faso and their comparison to human Salmonella isolate. Salmonella. BMC Microbiology 13: 253.
  24. Long, M., Yu, H., Chen, L., Wu, G., Zhao, S., Deng, W., Chen, S., Zhou, K., Liu, S., He, l., Ao, X., Yan, Y., Ma, M., Wang, H., Davis M. A., Jones, L., Li, B., Zhang, A., Zou, L., 2017. Recovery of Salmonella isolated from eggs and the commercial layer farms. Gut Pathogens, 9: 74.
  25. Marshall, B. M., Levy, S. B., 2011. Food Animals and Antimicrobials: Impacts on Human Health. Clinical Microbiology Reviews, 718–733.
  26. Mir, I. A., Kashyap, S. K., Maherchandani, S., 2015. Isolation, serotype diversity and antibiogram of Salmonella enterica isolated from different species of poultry in India. Asian Pacific Journal of Tropical Biomedicine, 5: 561-567.
  27. Naik, V. K., Shakya, S., Patyal, A., Gade, N. E., 2015. Isolation and molecular characterization of Salmonella spp. from chevon and chicken meat collected from different districts of Chhattisgarh, India. Veterinary World, 8: 702-706.
  28. Padron, M., 1990. Salmonella typhimurium outbreak in broiler chicken flocks in Mexico. Avian Diseases, 34(1): 221-3.
  29. Petra, L., 2009. Cross-contamination versus undercooking of poultry meat or eggs -which risks need to be managed first? International Journal of Food Microbiology, 134: 21-28.
  30. Popoff, M. Y., Bockemuhl, J., Gheesling, L. L., 2004. Supplement 2002 (no. 46) to the Kauffmann-White scheme. Research Microbiology. 155: 568-570.
  31. Singh, I. R., Yadav, A.S., Tripathi, V., Singh R.P., 2013. Antimicrobial resistance profile of Salmonella present in poultry and poultry environment in north. Food Control Journal, 33: 545-548.
  32. Singh, S, Yadav, A. S., Singh, S. M., Bharti, P., 2010. Prevalence of Salmonella in chicken eggs collected from poultry farms and marketing channels and their antimicrobial resistance. Food Research International, 43: 2027–2030.
  33. Stevens, A., Kaboré, Y., Perrier-Gros-Claude, J-D., Millemann, Y., Brisabois, A., Catteau, M., Cavin, J-F., Dufour, B., 2006. Prevalence and antibiotic-resistance of Salmonella isolated from beef sampled from the slaughterhouse and from retailers in Dakar (Senegal). International Journal of Food Microbiology, 110: 178–186.
  34. Suresh, T., Aam, H., Harsha, H. T., Lakshmanaperumalsamy, P., 2011. Prevalence and distribution of Salmonella serotypes in marketed broiler chickens and processing environment in Coimbatore City of southern India. Food Research International, 44: 823-825.
  35. Tessema, K., Bedu, H., Ejo, M., Hiko, A., 2017. Prevalence and Antibiotic Resistance of Salmonella Species Isolated from Chicken Eggs by Standard Bacteriological Method. Journal of Veterinary Science and Technology, 8: 1.
  36. Traoré, O., Nyholm, O., Siitonen, A., Bonkoungou, I. J. O., Traoré, A. S., Barro, N., Haukka, K., 2015. Prevalence and diversity of Salmonella enterica in water, fish and lettuce in Ouagadougou, Burkina Faso. BMC Microbiology, 15: 151.
  37. Van Duijkeren, E., Wannet, W. J. B., Houwers, D. J., Van Pelt, W., 2002. Serotype and Phage Type Distribution of Salmonella Strains Isolated from Humans, Cattle, Pigs, and Chickens in The Netherlands from 1984 to 2001. Journal of Clinical Microbiology, 3980-3985.
  38. Yildirim, Y., Gonulalan, Z., Pamuk, S., Ertas, N., 2011. Incidence and antibiotic resistance of Salmonella spp. on raw chicken carcasses. Food Research International, 44: 725–728.
  39. Yoke-Kqueen, C., Learn-Han, L., Noorzaleha, A. S., Son, R., Sabrina, S., Jiun-Horng, S., Chai-Hoon, K., 2008. Letters in Applied Microbiology, 46: 318–324.
  40. Zhao, S., Mcdermott, P. F., Friedman, S., Abbott, J., Ayers, S., Glenn, A., Hall-Robinson, E., Hubert, S. K., Harbottle, H., Walker, R. D., Chiller, T. M., White, D.G., 2006. Antimicrobial Resistance and Genetic Relatedness Among Salmonella from Retail Foods of Animal Origin: NARMS Retail Meat Surveillance. Foodborne pathogens and disease, 3, (1): 106-17.

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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