Biosurfactants are amphipathic compounds produced extracellularly by microorganisms on cell surfaces, or excreted extracellularly. They contain hydrophilic and hydrophobic moieties that reduce surface and interfacial tension between molecules at the surface and interface respectively. The present study focused on isolation, screening, identification of biosurfactant producing bacteria and also development of economical methods for biosurfactant production by the use of unconventional substrates. The research investigated the potential of utilizing agro industrial (Pineapple and cassava) wastes to replace synthetic media for biosurfactant production. Eighteen isolates obtained were screened for biosurfactant activity using Emulsification Index, haemolysis and oil displacement method. The bacteria Pseudomonas fluorescens Strain PC20 isolated was able to grow and produce biosurfactant 10ml and 5ml per 200ml of cassava and pineapple peel respectively.
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