Cloning and Characterization of Undaria pinnatifida Suringar Phytoene Desaturase Gene Enhancing Carotenoid Accumulation in Transgenic Tobacco

Cloning and Characterization of Undaria pinnatifida Suringar Phytoene Desaturase Gene Enhancing Carotenoid Accumulation in Transgenic Tobacco

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Author(s): Tengteng Guan, Tengteng Guan, Hongmin Xu, Yi Ma, Yinjie Li

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DOI: 10.18483/ijSci.2359 37 216 1-5 Volume 9 - Jul 2020


The phytoene desaturase is a key enzyme involved in the carotenoid biosynthesis pathway, which catalyzes the conversion of ζ-carotene from phytoene. In this study, he PDS gene (UpPDS) was isolated and characterized from Undaria pinnatifida Suringar, the full-length cDNA sequence was 1707 bp in length and encoded 568 amino acid residues. Then, the plant expression vector pCAMBIA2300-UpPDS was constructed and transformed into Agrobacterium LBA4404, and then transferred into tobacco plants by infection. Transgenic tobacco plants were identified by PCR amplification and southern blotting. Assay of real-time quantitative PCR ananlysis indicated that the expression level of the target gene UpPDS differed greatly in different transgenic tobacco plants. Spectrophotometry was used to determine the carotenoids content in the leaves trangenic plants, and the results showed that the content of carotenoids in the leaves of transgenic plants was higher than that of wild tobacco, and the maximum content increased 1.13 times compared with that of wild plants.


Undaria pinnatifida Suringar, Phytoene Desaturase (PDS), Gene Cloning, Functional Expression, Transgentic Tabacoo


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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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