Quality Characteristics of Germinated Radish Seeds Treated with Illite Clay

Quality Characteristics of Germinated Radish Seeds Treated with Illite Clay

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Author(s): Il-Doo Kim, Dong-Young Im

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DOI: 10.18483/ijSci.2550 21 49 7-12 Volume 11 - Jan 2022


Radish (Raphanus sativus L.), a member of the cruciferous vegetable family, has been used as a medicinal food for a number of ailments. Radish contains a wide variety of phytochemicals that show antioxidative, antimutagenic, antiproliferative properties and functions in the induction of detoxification enzymes. The objective of this study was to examine the effect of illite treatment on the yield and nutrient value of radish sprouts. The yield, color appearance, and total mineral content of many of the illite-applied radish sprouts were improved compared to the control. Overall, lower concentrations of illite were found to be more appropriate to enhance the yield and nutritional values of radish sprouts.


Amino Acid, Illite, Mineral, Radish Sprout, Yield


  1. Ahanger MA, Agarwal RM (2017) Potassium up-regulates antioxidant metabolism and alleviates growth inhibition under water and osmotic stress in wheat (Triticum aestivum L). Protoplasma 254:1471–1486. https://doi.org/10.1007/s00709-016-1037-0
  2. Algar E, Ramos-Solano B, García-Villaraco A, et al (2013) Bacterial bioeffectors modify bioactive profile and increase isoflavone content in soybean sprouts (Glycine max var Osumi). Plant Foods Hum Nutr 68:299–305. https://doi.org/10.1007/s11130-013-0373-x
  3. Barillari J, Cervellati R, Paolini M, et al (2005) Isolation of 4-methylthio-3-butenyl glucosinolate from Raphanus sativus sprouts (Kaiware Daikon) and its redox properties. J Agric Food Chem 53:9890–9896. https://doi.org/10.1021/jf051465h
  4. Beevi SS, Mangamoori LN, Subathra M, Edula JR (2010) Hexane extract of Raphanus sativus L. roots inhibits cell proliferation and induces apoptosis in human cancer cells by modulating genes related to apoptotic pathway. Plant Foods Hum Nutr 65:200–209. https://doi.org/10.1007/s11130-010-0178-0
  5. Bhandal IS, Malik CP (1988) Potassium estimation, uptake, and its role in the physiology and metabolism of flowering plants. In: International Review of Cytology. Elsevier, pp 205–254
  6. Chen Y, Chang SKC (2015) Macronutrients, phytochemicals, and antioxidant activity of soybean sprout germinated with or without light exposure. J Food Sci 80:S1391–S1398. https://doi.org/10.1111/1750-3841.12868
  7. Ferreira CD, Bubolz VK, da Silva J, et al (2019) Changes in the chemical composition and bioactive compounds of chickpea (Cicer arietinum L.) fortified by germination. LWT 111:363–369. https://doi.org/10.1016/j.lwt.2019.05.049
  8. Ganz T (2013) Systemic iron homeostasis. Physiol Rev 93:1721–1741. https://doi.org/10.1152/physrev.00008.2013
  9. Gutiérrez RMP, Perez RL (2004) Raphanus sativus (radish): Their chemistry and biology. Sci World J 4:811–837. https://doi.org/10.1100/tsw.2004.131
  10. Hanlon PR, Webber DM, Barnes DM (2007) Aqueous extract from Spanish black radish (Raphanus sativus L. Var. niger) induces detoxification enzymes in the HepG2 human hepatoma cell line. J Agric Food Chem 55:6439–6446. https://doi.org/10.1021/jf070530f
  11. Harder H (1974) Illite mineral synthesis at surface temperatures. Chem Geol 14:241–253. https://doi.org/10.1016/0009-2541(74)90062-X
  12. Houston MC, Harper KJ (2008) Potassium, magnesium, and calcium: Their role in both the cause and treatment of hypertension. J Clin Hypertens 10:3–11. https://doi.org/10.1111/j.1751-7176.2008.08575.x
  13. Je J-Y, Park P-J, Jung W-K, Kim S-K (2005) Amino acid changes in fermented oyster (Crassostrea gigas) sauce with different fermentation periods. Food Chem 91:15–18. https://doi.org/10.1016/j.foodchem.2004.05.061
  14. Jribi S, Molnàr H, Antal OT, et al (2019) Zinc fortification as a tool for improving sprout hygienic and nutritional quality: a factorial design approach. J Sci Food Agric 99:5187–5194. https://doi.org/10.1002/jsfa.9765
  15. Kayahara H, Tsukahara K, Tatai T (2001) Flavor, health and nutritional quality of pre-germinated brown rice. In: Spanier AM, Shahidi F, Parliment TH, et al. (eds) Food Flavors and Chemistry: Advances of the New Millennium. Royal Society of Chemistry, Cambridge, UK, pp 546–551
  16. Kim I-D, Lee J-W, Kim S-J, et al (2014) Exogenous application of natural extracts of persimmon (Diospyros kaki Thunb.) can help in maintaining nutritional and mineral composition of dried persimmon. Afr J Biotechnol 13:2231–2239. https://doi.org/10.5897/AJB2013.13503
  17. Kitazaki S, Koga K, Shiratani M, Hayashi N (2012) Growth enhancement of radish sprouts induced by low pressure O2 radio frequency discharge plasma irradiation. Jpn J Appl Phys 51:01AE01. https://doi.org/10.1143/JJAP.51.01AE01
  18. Lee S-J, Ahn J-K, Khanh T-D, et al (2007) Comparison of isoflavone concentrations in soybean (Glycine max (L.) Merrill) sprouts grown under two different light conditions. J Agric Food Chem 55:9415–9421. https://doi.org/10.1021/jf071861v
  19. Lee S-O, Lee I-S (2006) Induction of quinone reductase, the phase 2 anticarcinogenic marker enzyme, in Hepa1c1c7 cells by radish sprouts, Raphanus sativus L. J Food Sci 71:S144–S148. https://doi.org/10.1111/j.1365-2621.2006.tb08917.x
  20. Lee Y-R, Lee H-S, Kim G-E, et al (2021) Effects of illite-containing fertilizer prototype on soil chemical property and tomato growth. Korean J Soil Sci Fertil 54:338–346
  21. Leigh RA, Wyn Jones RG (1984) A hypothesis relating critical potassium concentrations for growth to the distribution and functions of this ion in the plant cell. New Phytol 97:1–13. https://doi.org/10.1111/j.1469-8137.1984.tb04103.x
  22. Lintschinger J, Fuchs N, Moser J, et al (2000) Selenium-enriched sprouts. A raw material for fortified cereal-based diets. J Agric Food Chem 48:5362–5368. https://doi.org/10.1021/jf000509d
  23. Lisiewska Z, Słupski J, Skoczeń-Słupska R, Kmiecik W (2009) Content of amino acids and the quality of protein in Brussels sprouts, both raw and prepared for consumption. Int J Refrig 32:272–278. https://doi.org/10.1016/j.ijrefrig.2008.05.011
  24. Lugasi A, Blázovics A, Hagymási K, et al (2005) Antioxidant effect of squeezed juice from black radish (Raphanus sativus L. var niger) in alimentary hyperlipidaemia in rats. Phytother Res 19:587–591. https://doi.org/10.1002/ptr.1655
  25. Nakamura Y, Iwahashi T, Tanaka A, et al (2001) 4-(Methylthio)-3-butenyl isothiocyanate, a principal antimutagen in Daikon (Raphanus sativus; Japanese white radish). J Agric Food Chem 49:5755–5760. https://doi.org/10.1021/jf0108415
  26. Papi A, Orlandi M, Bartolini G, et al (2008) Cytotoxic and antioxidant activity of 4-methylthio-3-butenyl isothiocyanate from Raphanus sativus L. (Kaiware Daikon) sprouts. J Agric Food Chem 56:875–883. https://doi.org/10.1021/jf073123c
  27. Paucar-Menacho LM, Berhow MA, Mandarino JMG, et al (2010) Effect of time and temperature on bioactive compounds in germinated Brazilian soybean cultivar BRS 258. Food Res Int 43:1856–1865. https://doi.org/10.1016/j.foodres.2009.09.016
  28. Pocasap P, Weerapreeyakul N, Barusrux S (2013) Cancer preventive effect of Thai rat-tailed radish (Raphanus sativus L. var. caudatus Alef). J Funct Foods 5:1372–1381. https://doi.org/10.1016/j.jff.2013.05.005
  29. Pongrac P, Potisek M, Fraś A, et al (2016) Composition of mineral elements and bioactive compounds in tartary buckwheat and wheat sprouts as affected by natural mineral-rich water. J Cereal Sci 69:9–16. https://doi.org/10.1016/j.jcs.2016.02.002
  30. Shukla S, Chatterji S, Mehta S, et al (2011) Antidiabetic effect of Raphanus sativus root juice. Pharm Biol 49:32–37. https://doi.org/10.3109/13880209.2010.493178
  31. Skujins S (1998) Handbook for ICP-AES (Varian-Vista). A Short Guide to Vista Series ICP-AES Operation. Varian Int. AG, Zug, Switzerland
  32. Takaya Y, Kondo Y, Furukawa T, Niwa M (2003) Antioxidant constituents of radish sprout (Kaiware-daikon), Raphanus sativus L. J Agric Food Chem 51:8061–8066. https://doi.org/10.1021/jf0346206
  33. Taniguchi H, Kobayashi-Hattori K, Tenmyo C, et al (2006) Effect of Japanese radish (Raphanus sativus) sprout (Kaiware-daikon) on carbohydrate and lipid metabolisms in normal and streptozotocin-induced diabetic rats. Phytother Res 20:274–278. https://doi.org/10.1002/ptr.1851
  34. Udomkun P, Ilukor J, Mockshell J, et al (2018) What are the key factors influencing consumers’ preference and willingness to pay for meat products in Eastern DRC? Food Sci Nutr 6:2321–2336. https://doi.org/10.1002/fsn3.813
  35. Wagner AE, Terschluesen AM, Rimbach G (2013) Health promoting effects of brassica-derived phytochemicals: From chemopreventive and anti-inflammatory activities to epigenetic regulation. Oxid Med Cell Longev 2013:1–12. https://doi.org/10.1155/2013/964539
  36. Wang L-S, Sun X-D, Cao Y, et al (2010) Antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish (Raphanus sativus var. niger, Brassicaceae). Food Chem Toxicol 48:2712–2718. https://doi.org/10.1016/j.fct.2010.06.045
  37. Wang X, Yang R, Jin X, et al (2016) Effect of supplemental Ca2+ on yield and quality characteristics of soybean sprouts. Sci Hortic 198:352–362. https://doi.org/10.1016/j.scienta.2015.11.022
  38. Weaver CE (1965) Potassium content of illite. Science 147:603–605. https://doi.org/10.1126/science.147.3658.603
  39. Wei Y, Shohag MJI, Wang Y, et al (2012) Effect of zinc sulfate fortification in germinated brown rice on seed zinc concentration, bioavailability, and seed germination. J Agric Food Chem 60:1871–1879. https://doi.org/10.1021/jf205025b
  40. Wei Y, Shohag MJI, Ying F, et al (2013) Effect of ferrous sulfate fortification in germinated brown rice on seed iron concentration and bioavailability. Food Chem 138:1952–1958. https://doi.org/10.1016/j.foodchem.2012.09.134
  41. Xu N, Zou T, Pang JW, Hu GL (2012) Effects of exogenous zinc soaking on the seed germination, sprout growth of soybean and zinc accumulation in the sprouts. Soybean Sci 31:932–936
  42. Yamasaki M, Omi Y, Fujii N, et al (2009) Mustard oil in “Shibori Daikon” a variety of Japanese radish, selectively inhibits the proliferation of H-ras-transformed 3Y1 cells. Biosci Biotechnol Biochem 73:2217–2221. https://doi.org/10.1271/bbb.90322
  43. Yang H, Gao J, Yang A, Chen H (2015) The ultrasound-treated soybean seeds improve edibility and nutritional quality of soybean sprouts. Food Res Int 77:704–710. https://doi.org/10.1016/j.foodres.2015.01.011
  44. Yun J, Li X, Fan X, et al (2013) Growth and quality of soybean sprouts (Glycine max L. Merrill) as affected by gamma irradiation. Radiat Phys Chem 82:106–111. https://doi.org/10.1016/j.radphyschem.2012.09.004
  45. Zou T, Xu N, Hu G, et al (2014) Biofortification of soybean sprouts with zinc and bioaccessibility of zinc in the sprouts. J Sci Food Agric 94:3053–3060. https://doi.org/10.1002/jsfa.6658

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International Journal of Sciences is Open Access Journal.
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