Disturbed intestinal flora and leaky gut play the key roles in the occurrence of obesity-induced diabetes. Piperine can reduce the metabolic inflammation in obese mice and delay the occurrence of diabetes, but the mechanism is not clear. We aim to explore the potential mechanism of piperine in terms of intestinal barrier integrity and flora composition. Here, the obese mice model was induced by feeding 60% high-fat diet, and the feces of each mice were collected for 16s analysis of intestinal flora after intragastric administration of piperine (15 mg/kg/d) for 8 weeks. We found that piperine treatment decreased the serum contents of LPS, TNF-α and FFA, inhibited the high expression of inflammation-related proteins (TLR4, IL-1 β and CD11C) and upregulated the permeability-related proteins (ZO-1 and occludin) of ileum. Piperine also adjusted the ratio of Firmicutes to Bacteroides, changed the diversity and community richness of intestinal flora and regulated the content of short-chain fatty acids. Taken together, piperine can inhibit the occurrence of intestinal leakage by improving the disturbance of intestinal flora and the intestinal barrier integrity in obese mice.
Piperine, Intestinal Flora, Intestinal Barrier, Obesity, Diabetes
- Bordalo Tonucci L, Dos Santos KM, De Luces Fortes Ferreira CL, Ribeiro SM, De Oliveira LL, Martino HS. Gut microbiota and probiotics: Focus on diabetes mellitus. Critical reviews in food science and nutrition, 57 (2017) 2296-309. http:10.1080/10408398.2014.934438
- Blaut M, Clavel T. Metabolic diversity of the intestinal microbiota: implications for health and disease. The Journal of nutrition, 137 (2007) 751s-5s. http:10.1093/jn/137.3.751S
- Kau AL, Ahern PP, Griffin NW, Goodman AL, Gordon JI. Human nutrition, the gut microbiome and the immune system. Nature, 474 (2011) 327-36. http:10.1038/nature10213
- Hasain Z, Mokhtar NM, Kamaruddin NA, Mohamed Ismail NA, Razalli NH, Gnanou JV, et al. Gut Microbiota and Gestational Diabetes Mellitus: A Review of Host-Gut Microbiota Interactions and Their Therapeutic Potential. Frontiers in cellular and infection microbiology, 10 (2020) 188. http:10.3389/fcimb.2020.00188
- Amar J, Chabo C, Waget A, Klopp P, Vachoux C, Bermúdez-Humarán LG, et al. Intestinal mucosal adherence and translocation of commensal bacteria at the early onset of type 2 diabetes: molecular mechanisms and probiotic treatment. EMBO molecular medicine, 3 (2011) 559-72. http:10.1002/emmm.201100159
- Cani PD, Bibiloni R, Knauf C, Waget A, Neyrinck AM, Delzenne NM, et al. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes, 57 (2008) 1470-81. http:10.2337/db07-1403
- Mokkala K, Tertti K, Rönnemaa T, Vahlberg T, Laitinen K. Evaluation of serum zonulin for use as an early predictor for gestational diabetes. Nutrition & diabetes, 7 (2017) e253. http:10.1038/nutd.2017.9
- Demir E, Ozkan H, Seckin KD, Sahtiyancı B, Demir B, Tabak O, et al. Plasma Zonulin Levels as a Non-Invasive Biomarker of Intestinal Permeability in Women with Gestational Diabetes Mellitus. Biomolecules, 9 (2019). http:10.3390/biom9010024
- Vijayakumar RS, Nalini N. Piperine, an active principle from Piper nigrum, modulates hormonal and apo lipoprotein profiles in hyperlipidemic rats. Journal of basic and clinical physiology and pharmacology, 17 (2006) 71-86. http:10.1515/jbcpp.2006.17.2.71
- Yuan Y, Zhou J, Hu R, Zou L, Ji L, Jiang G. Piperine protects against pancreatic β-cell dysfunction by alleviating macrophage inflammation in obese mice. Life sciences, 274 (2021) 119312. http:10.1016/j.lfs.2021.119312
- Singer-Englar T, Barlow G, Mathur R. Obesity, diabetes, and the gut microbiome: an updated review. Expert review of gastroenterology & hepatology, 13 (2019) 3-15. http:10.1080/17474124.2019.1543023
- De Schepper S, Verheijden S, Aguilera-Lizarraga J, Viola MF, Boesmans W, Stakenborg N, et al. Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis. Cell, 175 (2018) 400-15.e13. http:10.1016/j.cell.2018.07.048
- Grigor'eva IN. Gallstone Disease, Obesity and the Firmicutes/Bacteroidetes Ratio as a Possible Biomarker of Gut Dysbiosis. Journal of personalized medicine, 11 (2020). http:10.3390/jpm11010013
- Liu C, Yuan Y, Zhou J, Hu R, Ji L, Jiang G. Piperine ameliorates insulin resistance via inhibiting metabolic inflammation in monosodium glutamate-treated obese mice. BMC endocrine disorders, 20 (2020) 152. http:10.1186/s12902-020-00617-1
Cite this Article:
International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.