Improvement of Piperine on the Disturbed Intestinal Flora and Leaky Gut in Obese Mice

Improvement of Piperine on the Disturbed Intestinal Flora and Leaky Gut in Obese Mice

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Author(s): Ruixin Hu, Ji Zhou, Guohui Jiang, Lixia Ji

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DOI: 10.18483/ijSci.2596 29 218 1-8 Volume 11 - Jul 2022


Disturbed intestinal flora and leaky gut play the key roles in the occurrence of obesity-induced diabetes. Piperine can reduce the metabolic inflammation in obese mice and delay the occurrence of diabetes, but the mechanism is not clear. We aim to explore the potential mechanism of piperine in terms of intestinal barrier integrity and flora composition. Here, the obese mice model was induced by feeding 60% high-fat diet, and the feces of each mice were collected for 16s analysis of intestinal flora after intragastric administration of piperine (15 mg/kg/d) for 8 weeks. We found that piperine treatment decreased the serum contents of LPS, TNF-α and FFA, inhibited the high expression of inflammation-related proteins (TLR4, IL-1 β and CD11C) and upregulated the permeability-related proteins (ZO-1 and occludin) of ileum. Piperine also adjusted the ratio of Firmicutes to Bacteroides, changed the diversity and community richness of intestinal flora and regulated the content of short-chain fatty acids. Taken together, piperine can inhibit the occurrence of intestinal leakage by improving the disturbance of intestinal flora and the intestinal barrier integrity in obese mice.


Piperine, Intestinal Flora, Intestinal Barrier, Obesity, Diabetes


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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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