Amygdala Kindling Alters Estrus Cycle and Ovarian Morphology in the Rat

Amygdala Kindling Alters Estrus Cycle and Ovarian Morphology in the Rat

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Author(s)

Author(s): P. Andy Li, Juan Pan, Lingwu Zhang, Feng Wang, Dan Liu, Tao Sun

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446 922 12-21 Volume 2 - Nov 2013

Abstract

The objective of this study is to explore the effects of amygdala kindling on estrus cycle and ovarian morphology. Thirty-five female rats at the age of 8 weeks were randomly designated to electrode kindled, sham-kindled, and normal controls. Kindled rats were implanted with kindling electrodes in the left basolateral amygdala and kindled by brief suprathreshold stimulations with a bipolar electrode. Estrous cycles were daily monitored through vaginal smears. Electrographic and behavioral seizures were recorded and ovarian morphology was evaluated by light and electron microscopies. Our results showed that the kindled rats lost their ovarian periodicity displayed significant ovarian enlargement. H&E staining revealed increased number of growing follicles and total follicles, as well as polycysts in the ovaries of the kindled animals compared to sham and control animals. Ultrastructural study detected numerous apoptotic granulosa cells in growing follicles and thecal cell hyperplasia with secretary granules in the thecal cells in the kindled rats. The results suggest that amygdala kindling is a risk factor for the development of polycystic ovary syndrome.

Keywords

amygdala kindling, endocrinology, epileptogensis, estrus cycle, ovarian morphology, polycystic ovary syndrome, reproductive system

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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