Viability of Explants Rhododendrons (Rhododendron L.) Depending on Sterilizing Compounds

Viability of Explants Rhododendrons (Rhododendron L.) Depending on Sterilizing Compounds

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Author(s)

Author(s): Elena Kutas, Aleksandr Veyevnik, Vladimir Titok, Lyubov Ogorodnik

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390 988 48-50 Volume 3 - May 2014

Abstract

Results are presented on the influence of sterilizing compounds upon the yield of viable explants of Rhododendron in sterilized culture. Yield of viable explants is dependent upon type of sterilizing compound, species belonging of plant and type of explant. Results show, that 0,1% solution of silver nitrate is a most effective compound for sterilization of seeds of 8 Rhododendron species (sterilization for 5 minutes) and 0,1% solution of sublimate and diacid (sterilization for 8 minutes) are most effective for sterilization of buds of 4 Rhododendron species.

Keywords

sterilizing compounds, seeds, buds, rhododendrons

References

  1. Cacas A, Lasa J (1986). Multiplication in vitro en reniolacha azucarera (Beta vulgaris L.) Tipo
  2. de explante y sistema de sterilization. Estac. exp. Aula Dei. 18: 51-56
  3. Kudina GA, Dovbysh NP (1990). Microclonal propagation of hybrid rose. Industrial botany: Perspectives of evalution: Proceedings of scientific conference of Donetsk botanical garden of HASU, 1990, Kyiv: 193-194 (in Russian)
  4. Raskauskas V, Kazlauskiene R, Gudaviciene N, Sirvydyte D (1989). Sterilinanciu medziagu ir mitybos terpiu parinkimas meristeminiu metodu dauginamoms orchidejoms. Scientific transactions of institutes of higher education of Lithuania. (Bulletin of biological sciences). 27: З6-42 (in Lethuanian)
  5. Sudhadevi AN, Nataraja K (1987). Establishment of plantlets in hypocotyl cultures of Dalbergia latifolia Roxb. Indian J. Forestry. 10(1): 1-6
  6. Tvartkiladze OK, Mezencev AV (1987). Method of propagation of tea in vitro: a.s. 1311673 USSR, MKI A01NZ/OD/ - N 3868024/ 30-15; Declaration. 10.01.1985. Published 12.01.1987. Inventions Bulletin. 19:15 (in Russian)
  7. Balakrishnamurthy G, Sree Rangasamy (1988). Regeneration of Banana plantlet in vitro culture of floral apices. Curr. Sci. (India). 57(5): 270-272
  8. Dychdala GR (1983). Chlorine and chlorine compounds. In: Block S.S. (Eds.). Disinfection, sterilization and preservation. 3rd ed. Philadelphia: Lea & Febiger: 157-182
  9. Turner FJ (1983). Hydrogen peroxide and other oxidant disinfectants In: Block S.S. (Eds.). Disinfection, sterilization and preservation. 3rd ed. Philadelphia: Lea & Febiger: 240-250
  10. Larson EL (1991). Alcohols. In: Block S.S. (Eds.).Disinfection, sterilization and preservation. 3rd ed. Philadelphia: Lea & Febiger: 191-203
  11. Achmedova JV (1999). Introduction of tissues of sugar beet into culture in vitro with the aim of microclonal propagation. In: Problems of theoretical and applied genetics in Kazakhstan: Materials of republic conference. Alma-Ata. 18-22 of November:117-118 (in Russian)
  12. Atroschenko G, Guseva GG, Cherepanova MA (1990). Clonal micropropagation of black carrants. In: Materials of conference of the young scientists and students. Leningrad Agricultural institute. Leningrad: 43-44 ( in Russian)
  13. Melnichuk MD, Pinchuk AP, Maurer VM (2004). Processes of callusformation and organogenesis of hybrids of poplar in culture in vitro. Biology. Biotechnology. 5(1):25-30 (in Ukraine)
  14. Shumichin SA (2004). Optimization of separate stages of microclonal propagation of dahlia cultural: sterilization of explants. Bulletin of Permskogo university. 2: 61-63 (in Russian)
  15. Rokityanskaya LS (2005). Search of effective methods of sterilization of ripe germs of barley. In: Biology is a science of XXI century. Puschino: 190 (in Russian)
  16. Kiyosue T, Kamada H, Harada H (1989). Induction of Somatic Embryogenesis from Carrot Seeds by Hypochlorite Treatment. Plant Tissue Cult. Lett. 6(3): 138-143
  17. Anderson WC (1975). Propagation of rhododendrons by tissue culture. Part 1. Development of culture medium for multiplication of shoots. Combined Proceedings of the International Plant Propagator's Society. 25: 1929-1935

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
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