Author(s)
Author(s): Jian-Zhong Zhang, Li Jing, Ru Yan, Xiang-Mei Cao, Qing-Ping He, P. Andy Li
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Volume 3 - Oct 2014
Abstract
Diabetes is an important modifiable risk factor for cerebral ischemic stroke. It increases infraction area and restrains astrocyte activation. Meanwhile, hyperglycemia increase lactic acid and decreases pH. Lactic acid may partially be accountable for the detrimental effects of diabetes on ischemic stroke. The objective of this study was to investigate the effect of lactic acid on astrocyte viability and to explore the potential mechanisms that lactic acid enhances astrocyte cell death. Astrocytes were challenged by various concentrations of lactic acid (0, 2.5, 5,10 and 15 mM). Cell viability and PERK pathway were examined. The results showed that lactic acid resulted in astrocyte death (p<0.05) and activation of GRP78 and PERK (p<0.05). It is concluded that lactate acidosis causes stress to the endoplasmic reticulum.
Keywords
Acidosis, Astrocyte, Lactic acid, GRP78, PERK
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