Emblica Officinalis Protects Against Sodium Arsenite Induced Hepatotoxicity & Nephrotoxicity In Rats

Emblica Officinalis Protects Against Sodium Arsenite Induced Hepatotoxicity & Nephrotoxicity In Rats

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Author(s): Arun Kumar, Raj Bala, Farida Bano, Ranjit Kumar, Mohammad Ali

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DOI: 10.18483/ijSci.652 378 1190 47-55 Volume 4 - Mar 2015


Arsenic in the present times has caused lots of health hazards to humans. In developing countries like Bangladesh and India the high prevalence of contamination, the isolation and poverty of the rural population, and the high cost and complexity of arsenic removal systems have imposed a programmatic and policy challenge on an unprecedented scale. Although in India, Arsenic poisoning in ground water in Gangetic belt especially the districts adjoining the river Ganges right from Eastern Uttar Pradesh, Bihar to West Bengal are the major problem of concern as due to which major health related problems are arising. To combat the present problem, a pre-clinical study was done on Charles foster rats. They were treated with Sodium arsenite for 8 weeks to make arsenic model and upon these arsenic pretreated rats Emblica officinalis was administered for 4 weeks to study the antidote effects of this plant. The plant not only eliminated the effects of arsenic but also reversed the normal physiological activity in the animal by normalising the activity of liver and kidney. The present study concludes that this novel plant possesses hepato-protective as well as nephron-protective activity against arsenic induced toxicity.


Sodium Arsenite, Emblica Officinalis, Antidote Effect, Rats


  1. Abernathy, C.O., Y.P. Liu, D. Longfellow, H.V. Aposhian, B. Beck, B. Fowler, R. Goyer, R. Menzer, T. Rossman, C. Thompson and W. Michael (1999) Arsenic: health effects, mechanisms of actions, and research issues. Environ. Hlth. Perspect., 107, 593-597. http://dx.doi.org/10.1289/ehp.99107593
  2. Ahmad, I., Mehmood Z. and Mohammad F. (1998) Screening of some Indian medicinal plants for their antimicrobial properties. J. Ethnopharmacol., 62: 183-193. http://dx.doi.org/10.1016/s0378-8741(98)00055-5
  3. Akhand AA, Du J, Liu W, Hossain K, Miyata T, Nagase F, Kato M, Suzuki H, Nakashima I (2002) Redox-Linked Cell Surface-Oriented Signaling for T-Cell Death. Antioxid Redox Signal, 4:445-454. http://dx.doi.org/10.1089/15230860260196236
  4. Anetor, J.I. (2002) Serum uric acid and standardized urinary protein: Reliable bioindicators of lead nephropathy in Nigerian lead workers. African J. Biomed. Res., 5, 19-24.
  5. Aposhian HV, Aposhian MM (2006) Arsenic Toxicology: Five Questions. Chem Res Toxicol; 19 : 1-60. http://dx.doi.org/10.1021/tx050106d
  6. Aposhian, H.V. and Aposhian MM (1989).Newer developments in arsenic toxicity J.Am. Coll. Toxicol., ; 8, 1297-1305. http://dx.doi.org/10.3109/10915818909009121
  7. Asmawi, M.Z., H. Kankaanranta, E. Moilaness and H. Vapaatalo, (1993) Anti-inflammatory activities of Emblica officinalis Gaertn. J. Pharmacol., 45: 581-584. http://dx.doi.org/10.1111/j.2042-7158.1993.tb05605.x
  8. Berthelot MPE (1859), Report Chim. Appl,; 2884.
  9. Bhattacharya, A., Chatterjee A., Ghosal S. and Bhattacharya S.K., (1999) Antioxidant activity of active tannoid principles of Emblica officinalis (Amla). Indian J. Exp. Biol., 37: 676-680.
  10. Bhattacharya, A., S. Ghosal and S.K. Bhattacharya, (2002) Antioxidant activity of tannoid principles of Emblica officinalis (amla) in chronic stress induced changes in rat brain. Indian J Exp Biol., 38(9): 877-80. http://dx.doi.org/10.1078/0944-7113-00090
  11. Bones RW. (1945) J Biol Chem, pp.; 158-581.
  12. Chakraborty D, Verma R (2010) Ameliorative effect of Emblica officinalis aqueous extract on ochratoxin-induced lipid peroxidation in the kidney and liver of mice. Int J Occup Med Environ Health 23:63–73. http://dx.doi.org/10.2478/v10001-010-0009-4
  13. Chandra Sekhar, K., Chary N.S., Kamala C.T., Venkatesware Rao J., Balaram V. and Anjaneya Y. (2003). Risk assessment and pathway study of arsenic in industrially contaminated sites of Hyderabad: A case study. Environ. Int., 29, 601-611. http://dx.doi.org/10.1016/s0160-4120(03)00017-5
  14. Colognato R, Coppede F, Ponti J, Sabbioni E, Migliore L (2007) Genotoxicity induced by arsenic compounds in peripheral human lymphocytes analysed by cytokinesis-block micronucleus assay. Mutagenesis, 22:255-261. http://dx.doi.org/10.1093/mutage/gem010
  15. Cui X, Kobayashi Y, Hayakawa T, Hirano S (2004) Arsenic speciation in bile and urine following oral and intravenous exposure to inorganic and organic arsenics in rats. Toxicol Sci, 82:478-487. http://dx.doi.org/10.1093/toxsci/kfh265
  16. Deokar A.B, (1998) Medicinal plant grown at Rajegaon. First ed. D.S Manav vikas foundation Pune,pp-48-49.
  17. Dioka, C.E., O.E. Orisakwe, F.A. Adeniyi and S.C. Meludu (2004). Liver and renal function tests in artisans occupationally exposed to lead in mechanic village in Nnewi, Nigeria. Int. J. Environ. Res. Pub. Hlth., 1, 21-25. http://dx.doi.org/10.3390/ijerph2004010021
  18. Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186:421- 31. http://dx.doi.org/10.1016/0076-6879(90)86135-i
  19. Fawcett JK, Scott JE. (1960) “A rapid and precise method for the determination of urea”. J Clin Pathol, 13(2)156-159. http://dx.doi.org/10.1136/jcp.13.2.156
  20. Ghosal S, Tripathi VK, Chauhan S. (1996) Active constituents of Emblica officinalis. Part I. The chemistry and antioxidative effects of two new hydrolysable tannins, emblicanin A and B. Indian J Chem Sec B;35:941–8. http://dx.doi.org/10.1002/chin.199647279
  21. Ghosh A, Sharma A, Talukdar G. (1992) Relative protection given by extract of Phyllanthus emblica fruit and an equivalent amount of vitamin C against a known clastogen— caesium chloride. Food Chem Toxicol;30:865-9. http://dx.doi.org/10.1016/0278-6915(92)90052-m
  22. Halliwell, B., (1997) Antioxidants and human diseases: A general introduction. Nutr. Rev., 55: S44-S49. http://dx.doi.org/10.1111/j.1753-4887.1997.tb06100.x
  23. Hossain K, Akhand AA, Kato M, Du J, Takeda K, Wu J, Takeuchi K, Liu W, Suzuki H, Nakashima I (2000): Arsenite induces apoptosis of Murine T lymphocytes through membrane raft-linked signaling for activation of cJun amino-terminal kinase. J Immunol 165:4290-4297. http://dx.doi.org/10.4049/jimmunol.165.8.4290
  24. Jeena K.J., Joy K.L, Kuttan R.(1999) Effect of Emblica officinalis, Phyllanthus amarus and Picrorhizia kurroa on N-Nitrosodiethyl amine induced hepato- carcinogenesis. Cancer letter, 136:11-16. http://dx.doi.org/10.1016/s0304-3835(98)00294-8
  25. Jendrassik L, Grof P, (1938) Vereinfachte. Photometrische Methodenzur Bestimmung des Blubilirubins. Biochem Z ; 297, 81-89.
  26. Jhantola-Vormisto A,summanen J.,Kankaanranta H.,Vuorela H., Ashwani Z.M, Moilanen E.,(1997) Anti-inflammatory activity of extracts from leaf of Phyllantinus emblica. Plant medica 63, 518-524. http://dx.doi.org/10.1055/s-2006-957754
  27. Jose, J.K., G. Kuttan and R. Kuttan, (2001) Antitumour activity of Emblica officinalis. J. Ethnopharmacol., 75: 65-69. http://dx.doi.org/10.1016/s0378-8741(00)00378-0
  28. Kalia, K. and S.J.S. Flora (2005). Strategies for safe and effective therapeutic measures for chronic arsenic and lead poisoning. J. Occup. Hlth., 47, 11-21. http://dx.doi.org/10.1539/joh.47.1
  29. Kind, PRN, King, EJ (1954) Estimation of Plasma Phosphatase by Determination of Hydrolysed Phenol with Amino-antipyrine. J Clin Path; 7(4), 322-326. http://dx.doi.org/10.1136/jcp.7.4.322
  30. Kitchin KT. (2001) Recent advances in arsenic carcinogenesis: Modes of action, animal model systems, and methylated arsenic metabolites. Toxicol. Appl. Pharmacol. 172:249-261. http://dx.doi.org/10.1006/taap.2001.9157
  31. Klassen, C.D. (1996). Heavy metals and heavy metal antagonists. In: Goodman and Gilman’s the pharmacological basis of therapeutics (Eds.: J.G. Hardman, A.G. Gilman and L.E. Limbird). McGraw – Hill, New York. pp. 1649-1672.
  32. Kumar GS, Nayaka H, Dharmesh SM, Salimath PV. (2006) Free and bound phenolic antioxidants in amla (Emblica officinalis) and turmeric (Curcuma longa). Journal of Food Composition and Analysis;19:446-52. http://dx.doi.org/10.1016/j.jfca.2005.12.015
  33. Li X, Pi J, Li B, Xu Y, Jin Y, Sun G (2008) Urinary arsenic speciation and its correlation with 8-0HDG in Chinese residents exposed to arsenic through coal burning. Bull Environ Contam Toxicol, 81:406-411. http://dx.doi.org/10.1007/s00128-008-9471-0
  34. Liu DN, Lu XZ, Li BL, Zhou DX, Li FX, Zheng DH, Wang KH (1992) Clinical analysis of 535 cases of chronic arsenic poisoning from coal burning. Chin J Med, 31:560-562.
  35. Liu J, Zheng B, Aposhian HV, Zhou Y, Cheng ML, Zhang AH, Waalkes MP (2002) Chronic arsenic poisoning from burning high-arsenic-containing coal in Guizhou, China. Environ Health Perspect, 110:119-122. http://dx.doi.org/10.1289/ehp.02110119
  36. NRC (1999) Arsenic in Drinking Water. National Academy Press, Washington, DC.
  37. NRC (2001) Arsenic in Drinking Water. (2001 Update). National Academy Press, Washington, DC.
  38. Perry, L.M. (1980): Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses, pp. 149 –150. MIT Press, Cambridge. http://dx.doi.org/10.1007/bf02858311
  39. Rajak, S., S.K. Banerjee, S. Sood, A.K. Dinda, Y.K. Gupta, S.K. Guptaand S.K. Maulik, (2004) Emblica officinalis causes myocardial adaptation and protects against oxidative stress in ischemic-reperfusion injury in rats. Phytother Res., 18(1): 54-60. http://dx.doi.org/10.1002/ptr.1367
  40. Rege, N.N., U.M. Thatte and S.A. Dahanukar, (1999) Adaptogenic properties of six rasayana herbs used in Ayurvedic medicine. Phytother. Res., 13: 275-291. http://dx.doi.org/10.1002/(sici)1099-1573(199906)13:4%3C275::aid-ptr510%3E3.0.co;2-s
  41. Rehaily Al, A.J., Howiriny Al, T.A. Sohaibani Al M.O. and Rafatullah S. (2002) Gastroprotective effects of 'Amla' Emblica officinalis on in vivo test models in rats. Phytomedicine., 9(6): 515-22. http://dx.doi.org/10.1078/09447110260573146
  42. Reitmann S and Frankel S. (1957) “A colorimetric method for determination of serum glutamate oxalacetic and glutamic pyruvate transaminases.” Amer J Clin Path 28(1): 56-63.
  43. Rossman TG (2003) Mechanism of arsenic carcinogenesis: An integrated approach. Mutat Res, 533:37-66. http://dx.doi.org/10.1016/j.mrfmmm.2003.07.009
  44. Rossman, T.G., A.N. Uddin and F.J. Burns (2004) Evidence that arsenite acts as a cazuocarcinogen in skin cancer. Toxicol. Appl. Pharmacol., 198, 394-404.
  45. Sairam, K., C.V. Rao, M.D. Babu, K.V. Kumar, V.K. Agrawal and R.K. Goel, (2002) Antiulcerogenic effect of methanolic extract of Emblica officinalis: An experimental study. J. Ethnopharmacol., 82: 1-9. http://dx.doi.org/10.1016/s0378-8741(02)00041-7
  46. Scartezzini P, Antognoni F, Raggi MA, Poli F, Sabbiani C. (2006) Vitamin C content and antioxidant activity of the fruit and of the Ayurvedic preparations of Emblica officinalis Gaertn. J Ethnopharmacol;104:113–8. http://dx.doi.org/10.1016/j.jep.2005.08.065
  47. Sharma S.K., Perianyagam J.B.,Joseph A.,Christina A.J.M, (2003) anti-inflammetry activity of ethanol and aqueous extract of Emblica officinalis Gaerth fruits.Hamdard medicus XLVI, 71-73.
  48. Sies H (1986) Biochemistry of Oxidative Stress. Angew Chem Internat Ed Eng; 25,1058-71.
  49. Sordo, M., L.A. Herrera, P. Ostrosky-Wegman and E. Rojas (2001) Cytotoxic and genotoxic effects of As, MMA, and DMA on leukocytes and stimulated human lymphocytes. Teratog. Carcinog. Mutagen., 21, 249-260. http://dx.doi.org/10.1002/tcm.1013
  50. Sultana, S., S. Ahmad, N. Khanand T. Jahangir, 2005. Effect of Emblica officinalis (Gaertn) on CCl induced 4 hepatic toxicity and DNA synthesis in Wistar rats. Indian J Exp Biol., 43(5): 430-6
  51. Tasduq, S.A., P. Kaisar, D.K. Gupta, B.K. Kapahi, H.S. Maheshwari, S. Jyotsna, and R.K. Johri, (2005) Protective effect of a 50% hydroalcoholic fruit extract of Emblica officinalis against anti-tuberculosis drugs induced liver toxicity. Phytother Res., 19(3): 193-7. http://dx.doi.org/10.1002/ptr.1631
  52. Tchounwou, P.B., B. Wilson and A. Ishaque (1999) Important considerations in the development of public health advisories for arsenic and arsenic containing compounds in drinking water. Rev. Environ. Hlth., 14, 211-229. http://dx.doi.org/10.1515/reveh.1999.14.4.211
  53. Thilakchand KR, Mathai RT, Simon P, Ravi RT, Rao MPB and Baliga MS, (2013) Hepatoprotective properties of the Indian gooseberry (Emblica officinalis Gaertn): a review Food Funct., 1431-1441. http://dx.doi.org/10.1039/c3fo60237k
  54. Toro G.& Ackermann PG. (1975) Practical Clinical Chem; 154.
  55. Zhou Y-S, Du H, Cheng M-L, Liu J, Zhang X-J, Xu L (2002) The investigation of death from diseases caused by coal-burning type of arsenic poisoning. Chin J Endemiol 21:484-486.

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