Single and Combined Effects of Antibacterial Activities of Fruit and Stem Bark Extracts of Tetrapleura Tetraptera on Streptococcus Mutans and Pseudomonas Aeruginosa

Single and Combined Effects of Antibacterial Activities of Fruit and Stem Bark Extracts of Tetrapleura Tetraptera on Streptococcus Mutans and Pseudomonas Aeruginosa

Loading document ...
Page
of
Loading page ...

Author(s)

Author(s): G. M. Gberikon, Agbulu C.O, Adeoti I.I.

Download Full PDF Read Complete Article

DOI: 10.18483/ijSci.693 350 1172 46-49 Volume 4 - Apr 2015

Abstract

Single and combined effect of the antibacterial activities of fruit and stembark extracts of Tetrapleura tetraptera was carried out against Streptococcus mutans and Pseudomonas aeruginosa. Samples of T.tetraptera fruits were purchased in Wukari market, Taraba State. Stem bark samples were collected from Vandeikya, Benue State of Nigeria. Phytochemical analyses were carried out on both samples in the laboratory, Department of Biological Sciences, University of Agriculture, Makurdi. Phytochemical screening showed the presence of flavonoids, saponins, tannins, steroids, alkaloids, phlobatannins, anthraquinones, glycosides and reducing sugars in varying concentrations in fruit and stem bark samples. Antibacterial activity of extracts of fruit and stembark of Tetrapleura tetraptera singly and combined was studied against Pseudomonas aeruginosa and Streptococcus mutans. Results showed that extract of fruit of test plant gave an inhibition zone of 21.00mm against Pseudomonas aeruginosa, 08.33mm against S. mutans . Extract of stem bark gave 12.00mm against S. mutans and no antibacterial activity against Pseudomonas aeruginosa. Combined extracts of Tetrapleura tetraptera fruit and stembark showed antibacterial activity on Pseudomonas aeruginosa giving an inhibition zone of 16.67mm and S. mutans an inhibition zone of 10.33mm. There was significant difference (p˂ 0.025) between the antibacterial effects of extracts of fruit and stem bark of Tetrapleura tetraptera singly and combined as shown in this study. Minimum inhibitory concentration of test organisms subjected to single and combined extracts of fruit and stem bark was 100mg/ml except for P.aeruginosa which showed that concentrations (mg/ml) of stem bark extract used singly was not enough to inhibit its growth, except the combined extract which inhibited its growth at 100mg/ml.

Keywords

Pseudomonas aeruginosa, Streptococcus mutans Tetrapleura tetraptera, Phytochemicals flavonoids, saponins, tannins, steroids, alkaloids, phlobatannins, anthraquinones, glycosides, reducing sugar, antibacterial, ethanolic, combined, single

References

  1. Jorgensen, J. H., and J. D. Turnidge. (2003). Susceptibility test methods: Dilution and Disc diffusion methods. Manual of Clinical Microbiology. 8th ed. 1108 - 1127 55(3) 205-238, 243-251.
  2. Joy, P.P., Thomas, J., Mathew, S.S., and Karia, B.P.(1998). Medicinal Plants. Aromatic and Medicinal Research Station, Kerula India. Pp 1-3.
  3. Lai, P.K and Roy, J. (2004). Antimicrobial and chemo preventive properties of herbs and spices. Curr Med. Chem. 11(11):1451-1460 http://dx.doi.org/10.2174/0929867043365107
  4. Lichterman, B.L.(2004). “Aspirin: The Story of a Wonder Drug”. British Medical Journal. 329(7479):1408. http://dx.doi.org/10.1136/bmj.329.7479.1408
  5. Masud, S.M., Rana, M.P., Billah, M. Raju, G.S. (2014). Susceptibility of Microorganisms to selected Medicinal Plants in Banglandesh. Asian Pacific Journal of Tropical Biomedicine. 4(11) 911-917. http://dx.doi.org/10.12980/apjtb.4.201414b362
  6. Nostro, A., Germanŏ, M.P., D’Angelo, V., Marino, A. and Cannattelli, M.A. (2000): Extraction Methods and Bioautography for Evaluation of Medicinal plants for Antimicrobial activity. Lett. Appl. Microbiol ., 30:379-384. http://dx.doi.org/10.1046/j.1472-765x.2000.00731.x
  7. Odebiyi, O. O. and Sofowora, E. A. (1978). Phytochemical screening of Nigeria medicinal plants II Lloydia 41: 2234-246.
  8. Okerulu, I. O. and Ani, C. J. (2001). The phytochemical analysis and antibacterial screening of extracts of Terracarpidium conophorum. Journal of Chemical Society of Nigeria 26(1): 53-56.
  9. Salie, F.; Eagles, P. F. K. and Lens, H. M. J. (1996). Preliminary antimicrobial screening of four South African Asteraceae species. J. Ethnopharmacol., 52(1):27-33. http://dx.doi.org/10.1016/0378-8741(96)01381-5
  10. Uchechi, N. E. and Chigozie, F. O. (2010). Antibacterial activity of Tetrapleura tetraptera Taub. Pod extracts. International Journal of Pharma and Bio Sciences.p 734. Vol.1.
  11. Umeh, E. U., Oluma, H. O. A. and Igoli, J. O. (2005). Antibacterial screening of four local plants using an indicator- based microdilution technique. Afr. J. Trad. 2(3): 238-243.

Cite this Article:

  • BibTex
  • RIS
  • APA
  • Harvard
  • IEEE
  • MLA
  • Vancouver
  • Chicago

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Search Articles

Issue June 2019

Volume 8, June 2019


Table of Contents


Order Print Copy

World-wide Delivery is FREE

Share this Issue with Friends:


Submit your Paper