Download Full PDF
Read Complete Article
DOI: 10.18483/ijSci.803
~ 991
` 1290
a 68-74
Volume 4 - Aug 2015
Abstract
Twenty six isolates of Streptococcus mitis bacteria collected from clinical of dental disease in missan city , then analyzed between (October and December in 2014).The samples taken in order to determine the bacterial profile and antibiotic susceptibility . Isolates of Streptococcus mitis were tested against eight different antibiotics and crude of crude aqueous extracts from flowers of Matricaria chamomilla L., by a disk diffusion method. 100% of isolates were resistat to the Tetracycline ,84%resistant to Ampicillin , 96% resistant Nitrofurantion ,100% resistant to Optochin ,80% resistant to Bacitracin ,96% resistant to, 92% resistant to Vincomycin , 73% resistant to Benzypenicillin where found to be the least effective antibiotics against MDR Streptococcus mitis. Also the antimicrobial suscepitility of isolates where tested against crude of crude aqueous extracts from flowers of Matricaria chamomilla L. with different concentration (62,5, 125, 250 , 500 mg / ml) detected by agar well diffusion methods , the aqueous extracts from flowers of Matricaria chamomilla L. have more effective in 500 mg / ml . Finally cytotoxicity evaluating toward human RBC , the results revealed these crud extracts of Matricaria chamomilla L. have not any cytotoxicity in all concentration . The good antimicrobial potency of crud extracts of Matricaria chamomilla L. indicates the treatment of MDR Streptococcus mitis as an alternative to the costly antibiotic.
Keywords
Antibiotic, MDR Streptococcus mitis, Minimum Inhibitory Concentration (MIC), Matricaria Chamomilla L. Flowers
References
- Atlas,R; Parls , L. and Baron , A.(1995).Laboratory manual of experiential Microbiology .1 ed Mosby, Ine . Missouri
- Bancescu , G.. ; Dumitriu ,S. ; Defta , M. (2004). Susceptibility testing of Streptococcus mitis group isolates. Indian J Med Res 119 (Suppl) May 2004, pp 257-261.
- Chopde , N.;Jawale ,B. ; Phanade ,B.(2012). Microbial characterization and their relation with potential identification in patient with denture stomatitis ,J.of Contemporary Dental Practice , vol .13.pp.456-159. http://dx.doi.org/10.5005/jp-journals-10024-1168
- Doern ,G. ; Tiarans ,M. ; Brneggemann ,A.(1996).Emergence of high rate of antimicrobial resistance among viridans group Streptococcai in United States Antimicrob Agent. Chemother.40:891-4.
- Elliott ,T. ;Worthington ,T. ; Osman ,H. and Gill ,M. (2007). Medical Microbiology and infection 4ed , Blackwell Publishing Ltd, Oxford , UK.
- Ghitesen ,J. ; Bucharest , C. (1997). Antibiotherapy.
- Johan ,P.; Harley , I. ; Prescott ,M. (2003) . Laboratory Exercise in Microbiology .McGraw-Hill.USA.pp.484 ,149-1538.
- Jonathan EK, Anna KJ, Johannes VS. Zulu medicinal plants with antibacterial activity. J Ethnopharmacol. 2000; 69: 241-6. http://dx.doi.org/10.1016/s0378-8741(99)00147-6
- Saeki Y, Ito Y, Okuda K.( 1989). Antimicrobial action of natural substances on oral bacteria. Bull Tokyo Dent Col. 30:129-35.
- Kirby ,W. ; Bauer ,A. (1966). Antibiotic Susceptibility Testing by a standardized single disk method Am .J. Clin .Pathol.45(4)493-496.
- Lewis ,M. ; Parkhnrest , C. ; Douglas , C.(1995). Prevalence's of penicillin resistant bacteria in acute supportive oral infection .J. Antimicrobial Chemoiler . 35:785-91.
- Lambert , P. (2005). Bacterial resistance to antibiotic :Modified target sites, Adv. Drug. Delive . Rev,57 (10):1471-1485. http://dx.doi.org/10.1016/j.addr.2005.04.003
- NCCLS.(2002). Performance Standards for Antimicrobial Disk Susceptibility Testing .Twelfth information Supplement.
- Renneberg ,J. ; Niemann ,L. ; Gutsehik ,E. (1997). Antimicrobial suscepitipllity of 278 Streptococci blood isolates to seven antimicrobial agents . J.Antimicrob Chemother .30.135-40.
- Tadeg , H. ; Mohammed ,E. ; Asres , K. ; Geber-Mariam , T.(2005). Antimicrobial activities of some selected tradition Ethiopian medicinal plants used in the treatment of skin disorders .J.Ethanopharmacol.43:173-177. http://dx.doi.org/10.1016/j.jep.2005.02.031
- Xian-gou , H. and Ursula , M.(1994). Antifungal compound from Solanum nigrescens .J. Ethano Pharmacology . 43: 173-177. http://dx.doi.org/10.1016/0378-8741(94)90039-6
Cite this Article:
International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.