Author(s): Hong Yu, Jing Wang, YujingWang, Chunmei Liu
To investigate the effects of small interfering RNA (siRNA ) SIRT1 on the proliferation of human cervical cancer cells with the hopes of finding new diagnostic and therapeutic modalities. Chemical synthesis of siRNA targeted against SIRT1 were transfected into human cervical cancer C33A cells by Lipofectmine RNAi-MAX liposomes, on harvesting at 72h after transfection, the total RNA were extracted by the TRIZOL reagent and reverse transcribed into cDNA with the PrimeScript RT-PCR kit, and the expression level of SIRT1 mRNA was detected by RT-PCR. The cell proliferation was performed by CCK-8 method. RT-PCR results indicated that the mRNA expression of siRNA SIRT1 group was significantly down-regulated (P<0.05) compared to the control groups. CCK-8 proliferation assay showed that the inhibition rate of siRNA SIRT1 group was 53.1%, while the negative control group was 35.4%, the inhibition rate of transfection reagent control group was 11.5%. The inhibition rate of siRNA-SIRT1 group was significantly higher than other groups. Conclusion SIRT1 siRNA could down-regulate the expression of SIRTl mRNA, inhibit the proliferation of C33A cells, suggesting that SIRTl has promoting abilities in human cervical cancer.
SIRT1, RNAi, Cervical cancer cell
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