Use of Agro-Energy Waste as Agricultural Inputs: Impact on Soil Bacterial Diversity

Use of Agro-Energy Waste as Agricultural Inputs: Impact on Soil Bacterial Diversity

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Author(s)

Author(s): Bruno Oliveira de Carvalho, Rafael Antônio Presotto, Jéssica de Oliveira Lima, Shana de Mattos de Oliveira Coelho, Miliane Moreira Soares de Souza, Everaldo Zonta, Irene da Silva Coelho

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DOI: 10.18483/ijSci.1070 265 1082 124-130 Volume 5 - Jun 2016

Abstract

Waste reuse is a very interesting option from an economic, environmental, and often a social point of view. However, it is important to assess the impact of their use on soil characteristics to optimize proper dosages. Because microbial diversity can be used as one of the indicators of soil quality; we aimed to evaluate soil bacterial diversity after using combinations of gravel (waste from drilling in the petroleum industry) and Crambe press-cake (a by-product of biodiesel production) in haplic planosol cultivated with sunflowers (Helianthus annus l.) using a culture-independent technique, Denaturing Gradient Gel Electrophoresis (DGGE). Analysis of variance showed that the use of gravel and Crambe press cake at doses tested in haplic planosol did not significantly affect soil microbial diversity. This wastes had no significant effect on the number of bands obtained using DGGE. Besides, dynamics of the bacterial population did not follow any other parameter, such as chemical parameters, evaluated in the study, confirming that changes in the chemical characteristics of the soil due to the application of the wastes did not affect bacterial diversity. The results indicate that the use of these wastes is environmentally safe and that higher sodium content did not affect soil bacteria diversity.

Keywords

Crambe press cake, Denaturing Gradient Gel Electrophoresis (DGGE), microorganisms, Oil well drilling gravel, planosol.

References

  1. Araújo, A.S.F., Monteiro, R.T.R. (2007) Indicadores biológicos de qualidade do solo. Bioscience J 23(3).
  2. Breslauer, K.J., Frankm R., Blocker, H., Marky, L.A. (1986) Predicting DNA figduplex stability from the base sequence. Proc Natl Acad Sci USA 83, 3746­3750.
  3. Coyne, K.J., Hutchins, D.A., Hare, C.E., Cary, S.C. (2001) Assessing temporal and spatial variability in Pfiesteria piscicida distributions using molecular probing techniques. Aquat Microb Ecol 24, 275-285.
  4. Derakshani, M., Lukow, T., Liesack, W. (2001) Novel bacterial lineages at the (sub) division level as detected by signature nucleotide-targeted recovery of 16S rRNA genes from bulk soil and rice roots of flooded rice microcosms. Appl Environ Microbiol 67, 623-631.
  5. Diaz, H., Miska, S., Takach, N., Yu, M. (2004) Modeling of ECD in casing drilling operations and comparison with experimental and field data. In: IADC/SPE Drilling Conference. Society of Petroleum Engineers.
  6. Elsas, J.D.V., Smalla, K. (1995) Extraction of microbial community DNA from soils. In: Molecular Microbial Ecology Manual (pp. 61-71). Springer Netherlands.
  7. EMBRAPA – Empresa Brasileira de Pesquisa Agropecuária. (2013) Sistema Brasileiro de Classificação de Solos. 3ª Edição 353p.
  8. Fam, M.A., Dusseault, M.B. (1998) Borehole stability in shales: a physico-chemical perspective. In: SPE/ISRM Rock Mechanics in Petroleum Engineering. Society of Petroleum Engineers.
  9. Ferreira, E.P.B., Nunes, M.U.C., Xavier, G.R., Rumjanek, N.G. (2009) Perfis de PCR-DGGE de comunidades bacterianas associadas ao rizoplano de milho sob diferentes doses de adubação com compostos orgânicos. Bioscience J 25(3).
  10. Freitas, F.C. (2009) Dissertation. Uso de resíduo orgânico da produção de biodiesel direta da semente na atenuação dos efeitos de hidrocarbonetos de petróleo no solo. UFRRJ/IA
  11. Freitas, F.C.D., Presotto, R.A., Genúncio, G.D.C., Sobrinho, N.M.B.D.A., Zonta, E. (2015) pH, sodium, potassium, calcium, magnesium and aluminum in soils contaminated with petroleum drilling fluid after leaching tests. Ciência Rural 45, 1418-1423.
  12. Kane, D.J., Sarafian, T.A., Anton, R., Hahn, H., Gralla, E.B., Valentine, J.S., Bredesen, D.E. (1993) Bcl-2 inhibition of neural death: decreased generation of reactive oxygen species. Science 262, 1274-1277.
  13. Kirk, J.L., Beaudette, L.A., Hart, M., Moutoglis, P., Klironomos, J.N., Lee, H., Trevors, J.T. (2004) Methods of studying soil microbial diversity. J Microbiol Methods 58, 169-188.
  14. Magalhães, M.O.L., do Amaral Sobrinho, N.M.B., Zonta, E., de Carvalho, M.M., Tolón-Becerra, A. (2012) Effect of variations in the redox potential of Gleysol on barium mobility and absorption in rice plants. Chemosphere 89, 121-127.
  15. Marciano, C.R., Moraes, S.O., Oliveira, F.C., Mattiazzo, M.E. (2001) Efeito do lodo de esgoto e do composto de lixo urbano sobre a condutividade hidráulica de um Latossolo Amarelo saturado e não saturado. R Bras Ci Solo 25, 1-9.
  16. Muyzer, G., De Waal, E.C., Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59, 695-700.
  17. Oliveira, S.D. (2008) Dissertation. Avaliação das técnicas de bioaumento fúngico e bioestímulo em processos de biorremediação utilizando solo contaminado por petróleo. UFRJ/EQ.
  18. Ovreås, L., Forney, L., Daae, F.L., Torsvik, V. (1997) Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA. Appl Environ Microbiol 63, 3367-3373.
  19. Pires, A.M.M., Mattlazzo, M.E. (2008) Avaliação da Viabilidade do Uso de Resíduos na Agricultura. Circular Técnica, EMBRAPA, Jaguariúna, SP, 2008.
  20. Presotto, R.A. (2014) Dissertation. Efeito da Salinidade do Cascalho de Perfuração de Poços de Petróleo no Cultivo de Girassol. UFRRJ/IA.
  21. SAS Institute Inc. (2010) SAS OnlineDoc® 9.2.SAS Institute Inc
  22. Silva, R.B.D. (2013) Dissertation. Substituição de farelo de soja por torta de crambe para ovinos em crescimento. UFLA.
  23. Suzuki, M.T., Giovannoni, S.J. (1996) Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl Environ Microbiol 62, 625-630.
  24. Torsvik, V., Øvreås, L. (2002) Microbial diversity and function in soil: from genes to ecosystems. Curr Opin Microbiol 5, 240-245.
  25. Zilli, J.É., Rumjanek, N.G., Xavier, G.R., Costa, C.H.L., Neves, M.C.P. (2003) Diversidade microbiana como indicador de qualidade do solo. Cadernos de Ciência & Tecnologia, 20, 391-411.

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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