Isolation and Quantification of β-sitosterol, ergosterol and stigmasterol from Hypoxis rigidula Baker var. rigidula and Hypoxis hemerocallidea Fisch., C.A.Mey. & Avé-Lall (Hypoxidaceae)

Isolation and Quantification of β-sitosterol, ergosterol and stigmasterol from Hypoxis rigidula Baker var. rigidula and Hypoxis hemerocallidea Fisch., C.A.Mey. & Avé-Lall (Hypoxidaceae)

Loading document ...
Loading page ...


Author(s): Viresh Mohanlall, B. Odhav, N. Mkhize

Download Full PDF Read Complete Article

951 1820 118-134 Volume 2 - Jan 2013


Hypoxis rigidula Baker var. rigidula and Hypoxis hemerocallidea Fisch., C.A.Mey. & Avé-Lall (Hypoxidaceae) are frequently used African medicinal plant that has been used by traditional healers for daily healthcare needs. Phytosterols isolated from Hypoxis spp, have been found to be effective in lowering plasma cholesterol concentration by inhibiting the absorption of cholesterol in the small intestine. Phytosterols serve to stabilize phospholipid bilayers in plant cell membranes just as cholesterol does in animal cell membranes. The aim of this study was to isolate phytosterols from Hypoxis rigidula and evaluate its cholesterol reduction activity. The objectives were to extract phytosterols from Hypoxis using distillation; identify phytosterols using thin layer chromatography (TLC) and quantify phytosterols present in the plant extracts using high performance liquid chromatography (HPLC-UV). TLC gave the following phytosterol standard values: β-sitosterol at Rf 0.63±0, ergosterol at Rf 0.66±0 and stigmasterol at Rf 0.68±0. The chloroform extracts of both H. hemerocallidea and H. rigidula the presence of β-sitosterol, ergosterol and stigmasterol. HPLC analysis gave the following concentrations of phytosterols; 48.4 and 35.2 µg/ml of stigmasterol were found in H. rigidula and H. hemerocallidea respectively and 86.7 and 48.4 µg/ml of ergosterol were found in H. rigidula and H. hemerocallidea respectively. These results show a significant difference in the phytosterol content between the two species of the genus Hypoxis.


Hypoxis rigidula, Hypoxis hemerocallidea, phytosterols, secondary metabolites, cholesterol, .


  1. Bouic PJD (1996). Beta-sitosterol and beta-sitosterol glucoside stimulate human peripheral blood lymphocyte proliferation: implications for their use as an immunomodulatory vitamin combination. Int J Immunopharmacol. (18): 693-700
  2. Boukes GJ, Van der Venter M and Oosthuisen V (2008). Quantitative and qualitative analysis of sterols/sterolins and hypoxoside contents of three Hypoxis (African potato) spp. Afr.J. Biotechnol. 7 (11): 1624–1629
  3. Careri M, Elviri l and Mangia A (2001). Liquid- chromatography- UV determination and liquid chromatography atmospheric pressure chemical ionization mass spectrometric characterization of sitoterol and stimasterol in soy bean oil. J Chromatogr. (935): 249-257
  4. De-Eknamkul W and Potduang B (2003). Biosynthesis of β-sitosterol and stigmasterol in croton sublyratus proceeds via a mixed origin of isoprene units. Phytochemistry, (62): 389-398Jones PJH, Ntanios FY, Raeini-Sarajaz M and Vanstone CA (1999). Cholesterol-lowering efficacy ofa sitostanol-containing phytosterol mixture with a prudent diet in hyperlipidemic men. Am J Clin Nutr, (69): 1144-1150
  5. Lagarda MJ, Garcia-llatas G. and Farr´e R (2006). Analysis of phytosterols in foods. J Pharm Biomed Anal (41): 1486–1496.Marangoni F and Poli A (2010). Phytosterols and cardiovascular health. Pharmacol Res. (61): 193-199
  6. Moreau RA, Whitaker BD and Hicks KB (2002). Phytosterol, phytostanols and their conjugates infoods: structural diversity, quantitative analysis and health-promoting uses.Prog Lipid Res.41
  7. Nair VDP and Kanfer I (2008). Sterols and sterolins in Hypoxis hemerocallidea (african potato). S Afri J Sci. (104): 322-324
  8. Nair VDP, Kanfer I. and Hoogmartens J (2006). Determination of stigmasterol, β-sitosterol andstigmastanol in oral dosage forms using high performance liquid chromatography with evaporative light scattering detection. J Pharm Biomed Anal (41): 731-737
  9. Ojewole JAO (2006). Antinociceptive, anti-inflammatory and antidiabetic properties of hypoxis hemerocallidea fisch. & c.a mey. (hypoxidaceae) corm [african potato] aqueous extract in mice and rats. J Ethnopharmacol (103): 126-134
  10. Weber N, Weitkamp P and Mukherjee KD (2002). Cholesterol-lowering food additives: lipase catalysed preparation of phytosterol and phytostanol esters. Food Res Int (35): 177-181

Cite this Article:

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Search Articles

Issue June 2023

Volume 12, June 2023

Table of Contents

World-wide Delivery is FREE

Share this Issue with Friends:

Submit your Paper