Detection of Three Allelic Variants in the Rabbit Growth Hormone Gene

Detection of Three Allelic Variants in the Rabbit Growth Hormone Gene

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Author(s)

Author(s): A. C. Cattáneo, M. S. Trigo, L. O. Perez, C. A. Cordiviola, A. G. Antonini

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DOI: 10.18483/ijSci.1208 212 482 48-50 Volume 6 - Mar 2017

Abstract

Background: The aim of this study was to identify molecular variants in a DNA fragment of the rabbit growth hormone gene and assess their relative contribution in trials with different dietary protein sources and protein levels. We studied 52 rabbits from 22 matings at the Experimental Unit of the School of Agricultural Sciences and Forestry, National University of La Plata. Diets contained two protein levels (14 and 17%) and three protein sources (meat, fish and feather meal). We evaluated the different single nucleotide polymorphism (SNP) alleles and existing genotypes, and recorded the following productive variables: weaning weight (WW), weight at 45 days (W45), weight at slaughter (WS) and hot carcass weight (HCW). Results; Three SNP variants (c.-49C>A, c.-33A>G and c.66G>A) were detected in the DNA fragment analyzed with two alleles. Whereas c.-49C>A (CC, AC) and c.66G>A (GG, AG) had only two genotypes, and C-33A> G had the three possible ones. Molecular variants were in equilibrium and two showed association (p<0.001). The main contributor to overall phenotypic variation was HCW, followed by level and type of protein, and presence of c.-49C>A alleles associated with the growth hormone gene. Conclusions: The results reported in this study show the greater impact of changes in the levels and sources of protein in the diet on the percentage of variation in productivity than the presence or absence of a particular allele.

Keywords

Single nucleotide polymorphism, Animal Production, Genetics

References

  1. Ali H., Jafar S., Qurat-ul-Ain. (2016) Optimized genomic DNA extraction by a modified organic phenol-chloroform method without using PCR for best results. Int. J. Res. Med. Sci., 4, 100-104.
  2. Antonini A.G. (2007) Estrategia genética de producción, aptitud biológica y objetivos de selección en conejos para carne. Tesis. Facultad de Ciencias Veterinarias. UNLP. Available from: http://sedici.unlp.edu.ar/handle/10915/1584.
  3. Fontanesi, L., Dall'Olio, S., Spaccapaniccia, E., Scotti, E., Fornasini, D., Frabetti, A., Russo, V. A (2012) Single nucleotide polymorphism in the rabbit growth hormone (GH1) gene is associated with market weight in a commercial rabbit population. Livest Sci, Volume 147, Issue 1, 84 – 88.
  4. Garcia, J.F. (2006) Utilização de Marcadores Moleculares para a Seleção. 2 Simpósio Internacional de Reprodução Animal Aplicada, Londrina. 61, 115-123.
  5. Regitano, L.C.A. (2005) Genética Molecular Aplicada ao Melhoramento Reunião Anual da Sociedade Brasileira de Zootecnia, Goiânia, 30, 167-175.
  6. Van Laere, A.S., Nguyen, M., Braunschweig, M., Nezer, C., Collette, C., Moreau, L., Archibald, A. L., Haley, C. S., Buys, N., Tally, M., Andersson, G., Georges, M. y Andersson, L. (2003) A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature, 425, 832–836.
  7. Wallis, O.C., Wallis, M., (1995). Cloning and characterization of the rabbit growth hormone-encoding gene. Gene 163, 253–256.

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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