A New Exploration of Extracting and Purifying the Coenzyme F₄₂₀ from Natural Sludge

Author(s): ZHU Xiao-wen, LI Rong-gui

Coenzyme F₄₂₀ is one kind of special flavin cofactors which exist in some Archaea and bacteria, it is a low potential electron transfer carrier in methanogens. In this study, we designed three specific primers of F₄₂₀-dependent glucose-6-phos-phate dehydrogenase gene to determine which environment contains the more abundant coenzyme F₄₂₀, and methods for separating, extracting and purifying the coenzyme F₄₂₀ from this environmental samples were carried out, the pure coenzyme F₄₂₀ was obtained by some optimizing methods from the natural sludge , and the purity and concentration of coenzyme F₄₂₀ we got is just as good as the FMN standard sample which confirmed by their fluorescence emission spectrum. These optimizing methods would save more energy and time to get the good quality coenzyme F₄₂₀.

Coenzyme F₄₂₀, Separation and Purification, Fluorescence Spectra

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