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DOI: 10.18483/ijSci.1410
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Volume 6 - Aug 2017
Abstract
Six samples of honey from two states in Nigeria, Cross River and Oyo were investigated. (Igoli in Ogoja, farm bred and wild species from Obudu, Agoi Ibami, Eruwa and Iseyin) were analysed for their sugar components, Hydroxymethyl furfural (HMF), free acidity and electrical conductivity. Honey is composed of various sugars but the dominant sugars are two monosaccharide, fructose and glucose which are also reducing sugars. Honey is used for various purposes including medicinal, cosmetics, in the food industry, bakery and confectionary. The sugar contents were analysed using the HPLC with refractive Index (RI). The European Commission procedures were used to analyse HMF and electrical conductivity, while free acidity was analysed by AOAC methods (1999). The results of the analysis showed that although fructose and glucose were predominant in all the samples, only two samples, Igoli in Ogoja and Obudu farm bred met the Codex Alimentarius standard for fructose and glucose at 60g/100g. Fructose values (g/100g) reported for Igoli in Ogoja, Obudu farm, Agoi Ibami, Obudu wild, Eruwa and Iseyin were 34.92 ± 0.12, 36.45 ± 0.14, 30.50 ± 0.06, 28.45 ± 0.09, 33.40 ± 0.17, and 30.45 ± 0.09 respectively. Sucrose in all the honey samples met the Codex Alimentarius standard of < 5g/100g limit. Fructose/glucose ratio for all the samples was greater than 1 which indicated slower crystallization of all the honey samples. Values of free acidity ranged from 11.57 ± 0.07 to 18.60 ± 0.10meq/kg, which were lower than the 50meq/kg limit set by the Council of the European Union showing the absence of undesirable fermentation. HMF concentration in the samples all met the International optimum value of 40mg/kg reported by European Union and 80mg/kg for honey from the tropics. Electrical conductivity of the samples varied from 0.41 ± 0.01 to 0.63 ± 0.00mS/cm. The results showed that the honey samples sold locally in Nigeria meet the required international standards for honey.
Keywords
Sugars, Hydroxymethyl Furfural, Free Acidity, Electrical Conductivity
References
- Adams, B.A., Osikabor, B, Olomola, A.A and Adesope, A.A.A (2010) Analysis of physical and chemical composition of honey samples in selected markets in Ibadan Metropolis. Journal of Agriculture and Social Research 10(2)
- Adenekan, M. O., Amusa, N.A., Lawal, A. O and Okpeze, V.E (2010) Physico-Chemical and Microbiological properties of honey samples obtained from Ibadan. Journal of Microbiology and Antimicrobials. 2(8) 100 – 104
- Alosis, P.V. (2010) Determination of quality chemical parameters of honey from Chubut (Argentinean Patagonia). Chilean Journal of Agriculture Research 70(4). 640 – 645
- Amir, Y., Yesli, A., Bengana, M., Sadoudi, R and Amrouche, T (2010) Physico-Chemical and Microbiological assessment of honey from Algeria. Electronic Journal of Environmental, Agriculture and Food Chemistry 9(9) 1485 – 1494
- Alvarez-Suarez, J.M., Gasparrini, M., Forbes-Hernandez, L.M and Giamperr, F (2014) The Composition and Biological activity of honey. A focus on Manuka Honey. Journal of Foods. 3; 420 – 432
- vi(a). AOAC (1999) Official Methods of Analysis. 16th edition. 5th Revision. Vol II. Chapter 44, Sub-chapter 4. Cunnif P. (ed). Washington DC. USA
- vi(b). AOAC (2000) Sugars and Sugar products. In: Official Methods of Analysis. Horowitz, W. (ed). Association of Official Analytical Chemists International, vol. 2(44), 16th edition. Washington. DC. 22 – 33
- Ball, D. W. (2007) The chemical composition of Honey, Journal of Chemical Education 84(10): 1643 – 1646
- Bogdanov, S and Baumann (1988) Bestimmung von Honigzucker Mtch HPLC. Mitt Geblebensm Hygine 7a, 198 – 206
- Bodganov, S., Martin, P., Lullman, C., Borneck R., Flamini, Ch., Morlot, M., Heretier, J., Vorurohl, G., Russman, H., Persano-Oddo, L., Sabatini, A.G., Marcazzan, G.L., Marioleas, P., Tsigouri, K., Kerkvliet, J., Ortiz, A. and Ivanov, T. (1997) Harmonized methods of the European Honey Commission. Apidologie (extra issue) 1 – 59
- Bogdanov, S., Ruoff, R. and Persano-Oddo, L. (2004), Physicochemical methods of the characterization of unifloral honeys: A review. Apidologie 35: 4 – 17
- Bogdanov, S. (2009) Honey Composition. In: Book of Honey, Chapter 5, Bee Product Science.
- Buba, F., Gidado, A. and Shugaba, A. (2013). Analysis of chemical composition of Honey samples from North-East Nigeria. Biochemistry and analytical Chemistry. 2(139)
- Canterelli, M.A., Pellerano, R.G., Marchevsky, E.J. and Camina, J.M. (2008). Quality of Honey from Argentina. Study of chemical composition and trace elements. Journal of the Argentine Chemical Society: 96(1-2), 33 – 41
- Chaker, A., Ramane, A., Marcazzah, G.L. and Ferrazi, P. (2016). Physicochemical properties of some honey produced from different plants in Morocco. Arabian Journal of Chemistry. 9(2) 946 – 954
- Codex Alimentations (2001) Draft Revised Standard for honey (at step 10 of the Codex Procedure) Alinorm 01(25) 19 – 26
- Dike, O. N. and Onwuka, E. (2016) Entrepreneur Perception and Growth of Beekeeping in Abia State. Nigeria. International Journal of Physical and Human Geography. 4(3) 1 – 11
- Downey. G., Hussey, K., Kelly, J.D., Walshe, T.F. and Martin, P.G. (2005) Preliminary contribution to the characterization of artisanal honey produced on the Island of Ireland by palynological and physicochemical data. Food Chemistry, 91: 347 – 354
- Draiaia, R., Chefrour, A., Dainese, N., Borin, A., Manzinello, C., Gallina, A. and Mutinelli, F. (2015) Physico-Chemical parameters and antibiotics residuals in Algerian honey. African Journal of Biotechnology 14(14) 1242 – 1251
- Eteraf-Oskouei, T. and Najafi, M. (2013) Traditional and Modern Uses of Natural Honey in Human Diseases: A Review. Iranian Journal of Basic Medical Sciences. 16(6): 731 – 742
- El-Metwaly, A.A.E. (2015) Factors affecting the physical and chemical characteristics of Egyptian Beehoney. Ph.D Thesis. Faculty of Agriculture. Cairo University, Cairo. Egypt. 320p
- El-Sahaimy, S.A., Masry, S.H.D. and Shehata, M.G. (2015). Physicochemical characteristics of honey from different origins. Annals of Agricultural Sciences: 60(2), 279 – 287
- European Union Council (2002) Council direction 2001/110/EC of 20 December 2001 relating to Honey. Official Journal of European Communities L. 10: 47 – 52
- Ezekiel, A.A., Olagunju, F.I. and Olapade-Ogunwole, F. (2013). Economics of Honey production in Oyo State, Nigeria. Global Advanced Research. Journals of Arts and Humanities. Vol 2(2): 43 – 47
- Fasasi, K.A. (2012) Physicochemical attributes of Nigerian natural honey from honey bee (Apus mellifera adansonii) (Hymenoptera Alpedea) and its shelf life in storage at room temperature. Pakistan Journal of Biological Sciences. 15: 1027 – 1033
- Garcia, M.C., Perez, A.C. and Harrara, A. (2001). Pollen analysis and antibacterial activity of Spanish Honeys. Food Science Technology International 7: 155 – 158
- Human Metabolic Database (2007). http://hmdb.ca/metabolites/HMDB11742. Created 03/03/2009, updated 02/03/2017, cited 19/06/2017
- Kamal, M.A. and Klein, P. (2010). Determination of sugars in honey by liquid chromatography. Saudi Journal of Biological Sciences. Vol 18(1), 17 – 21
- Santos, E.K.G., Filho, A.N.D., Leite, R.H.L., Aroucha, E.N.M., Santos, A. G. and Oliveira, T.A. (2014) Rheological and some physico-chemical characteristics of some floral honey from plants of Caatinga. Anais da Academia Brasileira de Ciencias. 86(2)
- Subramanian, R., Umesh Hebbar, H. and Rastogi, N.K. (2007) Processing of Honey: A review. International Journal of Food Properties. 10(1): 127 – 143
- Terrab, A., Diez, M.J. and Heredia, F.J. (2003) Palynological, physico-chemical and colour characterization of Moroccan honey: Orange (Citrus spp) honey. International Journal of Food Science and Technology, 38: 387 – 394
- White, J.W. and Doner, L.W. (1980) Honey composition and properties of beekeeping in the United States. Agriculture Handbook number 335: 82 – 91. Published by the Science and Education Administration. Eastern Regional Research Centre. Philadelphia. USA
- Yadata, D. (2014) Detection of the electrical conductivity and acidity of honey from different areas of Tepi. Journal of Food Science and Technology. 2(5): 59 – 63.
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