In vitro Management of Bipolaris oryzae the Causal Pathogen of Brown Spot of Rice by Plant Extracts

In vitro Management of Bipolaris oryzae the Causal Pathogen of Brown Spot of Rice by Plant Extracts

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Author(s)

Author(s): NIKIEMA Wendyam Fabrice, KOÏTA Kadidia, ZIDA Elisabeth Pawindé, KABORE Koudougou Blaise

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DOI: 10.18483/ijSci.1503 152 482 42-47 Volume 6 - Dec 2017

Abstract

The antifungal potential of aqueous roots extracts of Securidaca longepedonculata and essential oil from leaves and flowering buds of Lippia multiflora against Bipolaris oryzae, causal agent of brown spot of rice was evaluated. Conidia of B. oryzae (Breda de Haan) Shoem, isolated on naturally infected leaves of the variety FKR19. The efficacy of the extracts to inhibit fungal growth was tested using well diffusion method onto PDA (Potato Dextrose Agar) growth medium in Petri plates. Results were record from the 5rd day until complete growth in control plates. The fresh root extracts of Securidaca longepedonculata (10% and 15%) significantly reduced the radial growth of the fungus with 42.49% and 72.56% efficiencies rates respectively. On the other hand, the dried extracts of the roots of the same plant at 15% and 20% concentrations with an efficiency of 4.81% and 14.44% respectively did not significantly reduced the radial growth of B oryzae. Essential oil of Lippia multiflora at 0.01% concentration significantly reduced the growth of the fungus to 62.64%, and the concentration of 0.1% completely inhibited its growth with 100% efficiency. The present study reveals that there is an alternative to chemicals through the use of local plant extracts that have antifungal properties capable of protecting the rice plant against possible attack of Bipolaris oryzae.

Keywords

Bipolaris oryzae, radial growth, antifungal potential, Lippia multiflora, Securidaca longepedonculata

References

  1. CIRAD-GRET (Center for International Cooperation in Agricultural Research for Development- GRET). (2002): Memento de l’agronome. CIRAD. 1691p.
  2. MAHRH (Ministry of Agriculture and Water Resources). (2005): Study on the marketing of rice. Rainfed rice project. Principal rapport volume 1. 71p.
  3. DGESS (General Direction of Studies and Sectoral Statistics). (2015): Final national cereal balance of the campaign (Burkina Faso).64 p.
  4. Traore A. and Yonly D. (2001): Weed competition in rainfed rice: Determining the optimal period of weeding in eastern Burkina Faso. Science et Technique, Sciences naturelles et agronomie, vol.25, n°1, pp.16-19.
  5. CNRST (National Center for Scientific and Technological Research). (2012): How to fight against rice blast, Technical Specifications, Research Programs and the support Development- Department of innovations and information systems, 2p.
  6. Agarwal C. P. Mortensen C. N. Mathur S. B. (1994): Diseases of rice transmitted by seeds and phytosanitary tests. CTA-ADRAO, ISBN 92 9081 1145, 95p.
  7. Ouedraogo I. (2001) : Etude de quelques isolats de Bipolaris oryzae, agent pathogène de l’helminthosporiose du riz au Burkina Faso. Degree of Diplôme d’Etudes Approfondies (DEA). University of Ouagadougou 03 BP 7021 Ouagadougou 03, Burkina Faso. 60p.
  8. Ouedraogo I. (2008) : Incidence de l’helminthosporiose du riz au Burkina Faso et caractérisation des populations de l’agent pathogène [Bipolaris oryzae ( Breda et Haan) Shoemaker]. Thesis state, UFR/SVT, University of Ouagadougou: Burkina Faso, 135p.
  9. Nebie C. H. R. Sereme A. Belanger A. Yameogo R. Sie F. S. (2002) : Etude des plantes aromatiques du Burkina Faso, caractérisations chimique et biologique des huiles essentielles de Lippia multiflora Moldenke. J. Soc. Ouest-Afr. Chim. 013; pp 27-37.
  10. Kagale S. Marimuthu T. Thayumanavan B. Nandakumar R. Samiyappan R. (2004): Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Datura metel against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae. Physiol. Mol. Plant Pathol. 65:91-100.
  11. Bakeer A. T. Khaled Elbanna K. and Elnaggar S.A. (2015): Impact of pre- and post-harvest applications of natural antimicrobial products on apple and pear soft rot disease. International Journal of Phytopathology 04(03):105-119.
  12. Koïta K. Neya B. F. Nana T. A. Zagre M. B. Sankara P.(2010) : Etude de l’efficacité d’extraits végétaux dans la lutte contre Cercospora arachidicola et Phaeoisariopsis personata (Berk et Curt.) au Burkina Faso. Annals of Ouagadougou University. Série C, Vol 008. 94-115.
  13. Soalla W. R. (2011) : Efficacité d'extraits aqueux de plantes contre les champignons
  14. pathogènes du niébé (Vigna unguiculata (L.) Walp.) au Burkina Faso. Master degree, Institute of Rural Development, University polytechnic of Bobo-Dioulasso, Burkina Faso. 59p.
  15. Bonzi S. Somda I.. Zida E. P. and Sereme P.. (2012): In vitro antifungal activity of various local plant extracts in the control of Phoma sorghina (Sacc.) Boerema et al. and Colletotrichum graminicola (Ces.) Wilson, as Sorghum Seed Mold Pathogen in Burkina Faso. Tropicultura 30 (2):103106.
  16. Zida P.E. Sereme P. Leth V. and Sankara P. (2008): Effect of aqueous extracts of Acacia gourmaensis A. Chev. and Eclipta alba (L.) Hassk. on seed health, seelding vigour and Grain yield of sorghum and pearl millet. Asian Journal of Plant Pathology 2 (1):40-47.
  17. Kabore K. B. Koïta E. Ouedraogo I. Nebie R. (2007): Efficacité d’extraits aqueux de plantes locales en traitement de semences contre la mycoflore du riz. Science et techniques, Vol.1, n°1. pp 49-57.
  18. Tiendrebeogo A. (2011) : Etude de l'efficacité des extraits de plantes locales (Eclipta alba L., Cymbopogon citratus (D.C.) Stapf, Agavae sisalana Perr. et Lippia multiflora Moldenke) contre les principaux champignons seminicoles du riz. Mémoire de fin de cycle, Institute of Rural Development, University polytechnic of Bobo-Dioulasso, Burkina Faso. 67p.
  19. Mathur S. B. Kongsdal O. (2003): Common laboratory seed health testing methods for detecting fungi. First edition, Kandrups Brogtrykkeri Publication, Denmark. ISBN: 3-906549-35-6. 425p.
  20. Khyade M. S. and Vaikos N. P. (2009): Antibacterial evaluation and phytochemical analysis of Wrightia tinctoria R. Br. Leaves. Pharmacology, 2, 808-813.
  21. Greche H. and Hajjani N. (2000). Chemical composition and antifungal properties of the essential oil of Tanacetum annuum. J. Oil Res., 12, 122-124.
  22. Dabire T. G. (2004) : Etude de l’efficacité d’extraits végétaux contre les agents pathogènes fongiques transmis par les semences de mil et de sorgho. Master degree. Institute of Rural Development, University polytechnic of Bobo-Dioulasso, Burkina Faso 75p.
  23. Montel S. (2004) : Evaluation de l’effet de quelques substances naturelles face aux champignons phytopathogènes les plus courants du riz. Internship report of BTS in vegetable productions. INERA, Farako-ba station. 44p.
  24. Koïta W.E. (2005) : Efficacité de quelques huiles essentielles et extraits aqueux de plantes locales contre certains agents pathogènes fongiques du riz. Master degree Institute of Rural Development, University polytechnic of Bobo-Dioulasso, Burkina Faso. 68p.
  25. Kintega K. R. (2014) : Proposition d’une méthode de lutte chimique contre Fusarium oxysoporium agent responsable de la fonte des semis et de la pourriture des bulbes d’oignon au champ. Professional Master in Plant Protection and Improvement. University of Ouagadougou. 80p
  26. Pousset J.L. (2004) : Plantes médicinales d’Afrique. Comment les reconnaître et les utiliser. Ed edisud, , 284 p.

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
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