Detection of Pasteurella Multocida by qPCR Associated with Pneumonic Lung in Pigs Slaughtered in Mato Grosso Brazil

Detection of Pasteurella Multocida by qPCR Associated with Pneumonic Lung in Pigs Slaughtered in Mato Grosso Brazil

Loading document ...
Page
of
Loading page ...

Author(s)

Author(s): Cristiane Silva Chitarra, Daphine Ariadne Jesus de Paula, Johanna Carolina Schvarzbach dos Santos, Ana Carolina Silva de Faria, João Xavier de Oliveira Filho, Marcelo Marques da Silveira, Anderson Castro Soares de Oliveira, Carolina Argenta Pescador, Luciano Nakazato, Valéria Dutra

Download Full PDF Read Complete Article

883 1630 25-31 Volume 2 - Apr 2013

Abstract

Pasteurella multocida is considered a important pathogens of swine respiratory system, causing progressive atrophic rhinitis, septicemia and pneumonia in pigs. The aim of this study is to quantify Pasteurella multocida in lungs of slaughtered pigs with and without pneumonia by qPCR technique and evaluate the interactions between bacteria and viruses by analyzing the association and the correlation between them using qPCR, microbiological and histopathological examinations of the lungs. Of the 109 samples collected, 61 samples showed no macroscopic lesions and 48 samples with macroscopic lesions. A total of 45.8% (22/48) of lung lesion group and 4.9% (3/61) of the group without lesions were positive for P. multocida isolation, being the only significant association with the lesion and control groups (p <0.000). Based on the number of copies of the kmt1 gene of P. multocida present in the lesion group (8.25 copies) and in the control group (1.6 copies) there is a significant difference (p <0.05). The qPCR technique for detection of P. multocida was efficient for being more sensitive than the microbiological method, demonstrating that a larger number of kmt1 gene copies is in GL suggest that P. multocida is an important agent in the development of pneumonia.

Keywords

Pasteurella multocida, pig, qPCR, swine respiratory complex

References

  1. ALLEN, T. C. (1992) Hematoxylin and eosin. In Laboratory methods in histotechnology. Ed E. B. PROPHET. Washington D. C, American Registry of Pathology. pp 53-58
  2. ANGEN, O., OLIVEIRA, S., AHRENS, P., SVENSMARK, B. & LESER, T. D. (2007) Development of an improved species specific PCR test for detection of Haemophilus parasuis. Vet Microbiol 119, 266-276
  3. BUSTIN, S. A., BENES, V., GARSON, J. A., HELLEMANS, J., HUGGETT, J., KUBISTA, M., MUELLER, R., NOLAN, T., PFAFFL, M. W., SHIPLEY, G. L., VANDESOMPELE, J. & WITTWER, C. T. (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55, 611-622
  4. CHATELLIER, S., HAREL, J., ZHANG, Y., GOTTSCHALK, M., HIGGINS, R., DEVRIESE, L. A. & BROUSSEAU, R. (1998) Phylogenetic diversity of Streptococcus suis strains of various serotypes as revealed by 16S rRNA gene sequence comparison. Int J Syst Bacteriol 48 Pt 2, 581-589
  5. FABLET, C., MAROIS, C., DORENLOR, V., EONO, F., EVENO, E., JOLLY, J. P., LE DEVENDEC, L., KOBISCH, M., MADEC, F. & ROSE, N. (2011) Bacterial pathogens associated with lung lesions in slaughter pigs from 125 herds. Res Vet Sci
  6. FENAUX, M., HALBUR, P. G., GILL, M., TOTH, T. E. & MENG, X. J. (2000) Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38, 2494-2503
  7. HANSEN, M. S., PORS, S. E., JENSEN, H. E., BILLE-HANSEN, V., BISGAARD, M., FLACHS, E. M. & NIELSEN, O. L. (2010) An Investigation of the Pathology and Pathogens Associated with Porcine Respiratory Disease Complex in Denmark. Journal of Comparative Pathology 143, 120-131
  8. HARMS, P. A., HALBUR, P. G. & SORDEN, S. D. (2002) Three cases of porcine respiratory disease complex associated with porcine circovirus type 2 infection. Journal of Swine Health and Production 10, 27-30
  9. MATTSSON, J. G., BERGSTROM, K., WALLGREN, P. & JOHANSSON, K. E. (1995) Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene. Journal of Clinical Microbiology 33, 893-897
  10. ONO, M., OKADA, M., NAMIMATSU, T., FUJII, S., MUKAI, T. & SAKANO, T. (2003) Septicaemia and Arthritis in Pigs Experimentally Infected with Pasteurella multocida Capsular Serotype A. Journal of Comparative Pathology 129, 251-258
  11. OPRIESSNIG, T., GIMÉNEZ-LIROLA, L. G. & HALBUR, P. G. (2011) Polymicrobial respiratory disease in pigs. Anim Health Res Rev 12, 133-148
  12. OPRIESSNIG, T., THACKER, E., YU, S., FENAUX, M., MENG, X. & HALBUR, P. (2004) Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneamoniae and porcine circovirus type 2. Veterinary Pathology 41, 624-640
  13. PALZER, A., RITZMANN, M., WOLF, G. & HEINRITZI, K. (2008) Associations between pathogens in healthy pigs and pigs with pneumonia. Veterinary Record 162, 267-271
  14. PIJOAN, C. (2006) Pneumonic Pasteurellosis. In Disease of Swine. 9 edn. Ed B. E. E. A. E. STRAW. Iowa, Iowa State University Press. pp 719-726
  15. PORS, S. E., HANSEN, M. S., CHRISTENSEN, H., JENSEN, H. E., PETERSEN, A. & BISGAARD, M. (2011) Genetic diversity and associated pathology of Pasteurella multocida isolated from porcine pneumonia. Vet Microbiol 150, 354-361
  16. QUINN, P. J., MARKEY, B. K., CARTER, M. E., DONNELLY, W. J. & LEONARD, F. C. (2002) Veterinary Microbiology and Microbial Disease, Blackwell Science Ltd
  17. ROSS, R. F. (2006) Pasteurella multocida and its role in porcine pneumonia. Animal Health Research Reviews 7, 13-29
  18. SAMBROOK, J. & RUSSEL, D. W. (2004) Molecular cloning A laboratory Manual. New York, Cold Spring Harbor laboratory Press
  19. SORENSEN, V., JORSAL, S. E. & MOUSING, J. (2006) Diseases of the respiratory system. In Diseases of swine. 9th edn. Ed B. STRAW, ZIMMERMANN, W., D’ALLAIRE, S., TAYLOR, D.J. Ames, Iowa State University Press. pp 149-177
  20. SUN, D., WANG, J., WU, R., WANG, C., HE, X., ZHENG, J. & YANG, H. (2010) Development of a novel LAMP diagnostic method for visible detection of swine Pasteurella multocida. Veterinary Research Communications 34, 649-657
  21. TOWNSEND, K. M., BOYCE, J. D., CHUNG, J. Y., FROST, A. J. & ADLER, B. (2001) Genetic organization of Pasteurella multocida cap Loci and development of a multiplex capsular PCR typing system. J Clin Microbiol 39, 924-929
  22. XIAO, G. S., CAO, S. J., DUAN, L. L., WEN, X. T., MA, X. P. & CHEN, H. M. (2006) Identification and Detection of Actinobacillus pleuropneumoniae in Infected and Subclinically Infected Pigs by Multiplex PCR Based on the Genes ApxIVA and OmlA. Agricultural Sciences in China 5, 146-154

Cite this Article:

  • BibTex
  • RIS
  • APA
  • Harvard
  • IEEE
  • MLA
  • Vancouver
  • Chicago

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Search Articles

Issue May 2019

Volume 8, May 2019


Table of Contents


Order Print Copy

World-wide Delivery is FREE

Share this Issue with Friends:


Submit your Paper