Modification of Periodic Acid Schiff and Haematoxylin Van Geison Solutions with Ethanol and Ethyl Acetate Wood Extract of Erythroxylum

Modification of Periodic Acid Schiff and Haematoxylin Van Geison Solutions with Ethanol and Ethyl Acetate Wood Extract of Erythroxylum

Loading document ...
Page
of
Loading page ...

Author(s)

Author(s): Nnaemeka OKORIE, Jessica Chioma OJUKWU, Samuel A. FASOGBON, Uchenna Oyibe OKEKPA, Adebukola Adisa EKOH-JOLLY, Cyril A. EGBO, Sumbola A. ADEYINKA, Samuel EHIREMEN, Odinaka Kingsley OBETA, Ibukun Akinsola OMISAKIN, Adekunle Oluwatosin ADELUWOYE

Download Full PDF Read Complete Article

DOI: 10.18483/ijSci.2567 14 52 13-19 Volume 11 - Apr 2022

Abstract

Background: In histopathology, stains are substances or biological dyes, which colour tissues in order to aid optical differentiation of tissue component. Erythroxylum (erythroxylon) also known as redheart wood, is a genus of tropical flowering plants in the family Erythroxylacae, It has been observed that it has bright-red color when freshly cut that darkens to deep red over time. Aim: The aim of this study is to determine the staining reaction and novel modification of periodic acid schiff and Haematoxylin Van Gieson solutions with ethanoic and ethylacetate wood extract of erythroxylum plant on kidney, liver, lung and intestinal tissue section of Rattus norvegicus as an alternative to Schiff reagent, compared with haematoxylin and eosin staining, Weigert’s iron haematoxylin Van gieson and Periodic acid Schiff staining method as control. Materials and Methods: Three serial sections were obtained from a block of a histological processed lung tissue of Rattus norvegicus and labelled A to C. Section A served as controls. Section As’ were stained with Haematoxylin and Eosin staining, Weigert’s Haematoxylin and Van Gieson Staining and Periodic acid Schiff reagent methods as controls. Test section Bs’were stained using Harris haematoxylin, Weigert’s Iron Haematoxylin, Periodic Acid and counter stained with Ethanol extract of erythroxylum solutions respectively. Tests Section Cs’ were all treated with the primary stained or oxidized with the required periodic acid and counter stained with ethylacetate extract of erthroxylum. Results: Liver sections treated with Periodic acid and counter stained with ethanol erythroxylum extract and ethylacetate erythroxylum solutions all revealed a comparable hepatic details as seen in periodic acid schiff solution. Similarly kidney sections of Rattus norvegicus stained with Weigert haematoxylin and counter stained with ethanol erythroxylum extract and ethylacetate erythroxylum extract solution also shown a comparable and better optical differentiation of the tubular structures and podocytes. Erythroxylum is a promising histological stain which is natural and readily available. Conclusion: The dye may serve in the demonstration of tissue constituents and serve as a useful cytoplasmic stain to replace other dyes for histological diagnosis of diseases.

Keywords

Dyes, Erythroxylum, Extract, Haematoxylin, Stain, Periodic acid

References

  1. Bieri, S., Bracket, A., Veuthey, J. and Christen, P. (2014). Cocaine distribution in wild Erythroxylum species. Journal of Ethnopharmacology, 103: 439-447.
  2. Restrepo, D., Ernesto, S., Orlando, A., Ivon, F., Sioly, R., Pablo, Z., Benjamin G, Juan A.D Auria J (2019): Erythroxylum in Focus; An Interdisciplinary Review of an overlooked molecules 24(20): 3788
  3. Meredith corpoeation [2020]; The Other Red wood; www.woodmagazine.com. Retrieved 20th October, 2021
  4. Plowman, T. and Hensold, N. (2004): Names, Types, Distribution of Neotropical Species of Erythroxylum (Erythroxylaceae): Britonia, 56:1-53
  5. Avwioro, O. (2002). Histochemistry and tissue Pathology Claverianumn Press Ibadan Nigeria, Pp. 134-213.
  6. Meior E (2020), Redheart; the wood database; retrieved from http;//www.wood-database. Com.redheart
  7. Ivelone M. de C. Barros, Bruno H. M. Leite, Caio F. M. Leite, Christopher W. Fagg , Sueli M. Gomes , Inês Sabioni Resck , Yris M. Fonseca-Bazzo , Pérola O. Magalhães , Dâmaris Silveira (2017). Chemical composition and antioxidant activity of extracts from Erythroxylum suberosum A.St. Hil.leaves. Journal of Applied Pharmaceutical Science. 7 (03): 088-094.
  8. Chung, R. and Brink, M. (2016). Erythroxylum (PROSEA Medicinal plants). Protologue: Civ not. Hist. Jamaica, 278:1756
  9. Okorie, N. (2021). Basic Histopathology Techniques Instructional Manual for Staff and Students, First edition 151(8) 156-160.
  10. Okorie N, Fasogbon S, Mohammed U, Abubakar U, Avwioro O, Tsamiya R (2020), Study of Phytochemicals Composition and Seperation of Colours using thun layers columns chromatography for Bixa orellana (annatto) seed extracts: Yenegoa Medical Journal, 2(1): 43-49.

Cite this Article:

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Search Articles

Issue October 2022

Volume 11, October 2022


Table of Contents



World-wide Delivery is FREE

Share this Issue with Friends:


Submit your Paper