Role of Macrophages in Internalization and Early Clearance of Pseudomonas Aeruginosa from the Mouse Lung

Role of Macrophages in Internalization and Early Clearance of Pseudomonas Aeruginosa from the Mouse Lung

Loading document ...
Page
of
Loading page ...

Author(s)

Massimo Conese, Miriam Margherita Cortese-Krott, Elena Copreni, Stefano Castellani, Sante Di Gioia

Download Full PDF Read Complete Article

Downloads: 582 Views: 937 Pages: 64-69 Volume 3 - Jan 2014

Abstract

This study aimed to evaluate the role of alveolar macrophages (AMs) in the internalisation and early clearance of Pseudomonas aeruginosa in the lung. AMs were depleted by intranasal administration of liposome-encapsulated clodronate disodium. At 24 h following the instillation of liposomes, a sublethal dose of P. aeruginosa (1 X 104 cfu) was inoculated intratracheally. Lung tissue was then evaluated for survival, viable bacteria and for histopathology 4 and 24 h post-infection. AM depletion of 90% did not affect the survival rate of infected mice but the clearance of P. aeruginosa was reduced at 24 h (P<0.05). While at 4 h AM-depleted and control mice showed similar level of PMN recruitment in the distal lung, 24 h post-infection tickening of interstitial spaces was more marked in AM-depleted mice. To evaluate the integrity of the epithelial barrier, the presence of bacteria in the spleen was assessed at 4 and 24 h. AM-depleted mice presented equal numbers of colonies as compared to control mice. At 4 h post-infection, the total and internalized numbers of bacteria were not different from the control group following a high-dose bacterial challenge (1 X 107 cfu). Our study show that AMs do not influence bacterial internalisation by airway epithelial cells.

Keywords

Acute Respiratory Infection, Airway Epithelial Cells, Alveolar Macrophages, Pseudomonas Aeruginosa

References

  1. Allewelt, M., Coleman, F. T., Grout, M., Priebe, G. P. & Pier, G. B. (2000). Acquisition of expression of the Pseudomonas aeruginosa ExoU cytotoxin leads to increased bacterial virulence in a murine model of acute pneumonia and systemic spread. Infect Immun 68, 3998-4004
  2. Bajmoczi, M., Gadjeva, M., Alper, S. L., Pier, G. B. & Golan, D. E. (2009). Cystic fibrosis transmembrane conductance regulator and caveolin-1 regulate epithelial cell internalization of Pseudomonas aeruginosa. Am J Physiol Cell Physiol 297, C263-277
  3. Broug-Holub, E., Toews, G. B., Van Iwaarden, J. F., Strieter, R. M., Kunkel, S. L., Paine III, R. & Standiford, T. J. (1997). Alveolar macrophages are required for protective pulmonary defenses in murine Klebsiella pneumonia: elimination of alveolar macrophages increases neutrophil recruitment but decreases bacterial clearance and survival. Infect Immun 65, 1139-1146
  4. Cheung, D. O. Y., Halsey, K. & Speert., D. P. (2000). Role of pulmonary alveolar macrophages in defense of the lung against Pseudomonas aeruginosa. Infect Immun 68, 4585-4592
  5. Conese, M., Cortese, M. M., Di Gioia, S. & Copreni, E. (2008). Role of Neutrophils in Internalisation and Early Clearance of Pseudomonas aeruginosa in the Mouse Lung. Int J Biomed Pharmaceut Sci 1, 140-143
  6. Hashimoto, S., Pittet, J. F., Hong, K., Folkesson, H., Bagby, G., Kobzik, L., Frevert, C., Watanabe, K., Tsurufuji, S. & Wiener-Kronish, J. (1996). Depletion of alveolar macrophages decreases neutrophil chemotaxis to Pseudomonas airspace infections. Am J Physiol Lung Cell Mol Physiol 270, L819-L828
  7. Hickman-Davis, J. M., Michalek, S. M., Gibbs-Erwin, J. & Lindsey, J. R. (1997). Depletion of alveolar macrophages exacerbates respiratory mycoplasmosis in mycoplasma-resistant C57BL mice but not mycoplasma-susceptible C3H mice. Infect Immun 65, 2278-2282
  8. Kannan, S., Huang, H., Seeger, D., Audet, A., Chen, Y., Huang, C., Gao, H., Li, S. & Wu, M. (2009). Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection. PLoS One 4, e4891
  9. Koh, A. Y., Priebe, G. P., Ray, C., Van Rooijen, N. & Pier, G. B. (2009). Inescapable need for neutrophils as mediators of cellular innate immunity to acute Pseudomonas aeruginosa pneumonia. Infect Immun 77, 5300-5310
  10. Kooguchi, K., Hashimoto, S., Kobayashi, A., Kitamura, Y., Kudoh, I., Wiener-Kronish, J. & Sawa, T. (1998). Role of alveolar macrophages in initiation and regulation of inflammation in Pseudomonas aeruginosa pneumonia. Infect Immun 66, 3164-3169
  11. Lavoie, E. G., Wangdi, T. & Kazmierczak, B. I. (2011). Innate immune responses to Pseudomonas aeruginosa infection. Microbes Infect 13, 1133-1145
  12. Manicone, A. M., Birkland, T. P., Lin, M., Betsuyaku, T., van Rooijen, N., Lohi, J., Keski-Oja, J., Wang, Y., Skerrett, S. J. & Parks, W. C. (2009). Epilysin (MMP-28) restrains early macrophage recruitment in Pseudomonas aeruginosa pneumonia. J Immunol 182, 3866-3876
  13. Mijares, L. A., Wangdi, T., Sokol, C., Homer, R., Medzhitov, R. & Kazmierczak, B. I. (2011). Airway epithelial MyD88 restores control of Pseudomonas aeruginosa murine infection via an IL-1-dependent pathway. J Immunol 186, 7080-7088
  14. Schroeder, T. H., Reiniger, N., Meluleni, G., Grout, M., Coleman, F. T. & Pier, G. B. (2001). Transgenic cystic fibrosis mice exhibit early clearance of Pseudomonas aeruginosa from the respiratory tract. J Immunol 166, 7410-7418
  15. Trotta, T., Di Gioia, S., Piro, D., Lepore, S., Cantatore, S., Porro, C., Castellani, S., Petrella, A., Fortunato, F., Maffione, A. B. & Conese, M. (2012). Effect of acute lung injury on VLA-4 and CXCR4 expression in resident and circulating hematopoietic stem/progenitor cells. Respiration 85, 252-264
  16. Tsai, W. C., Strieter, R. M., Mehrad, B., Newstead, M. W., Zeng, X. & Standiford, T. J. (2000). CXC chemokine receptor CXCR2 is essential for protective innate host response in murine Pseudomonas aeruginosa pneumonia. Infect Immun 68, 4289-4296
  17. Van Rooijen, N. & Sanders, A. (1994). Liposome mediated depletion of macrophages: mechanism of action, preparation of liposomes and applications. J Immunol Methods 174, 83-93
  18. Williams, B. J., Dehnbostel, J. & Blackwell, T. S. (2010). Pseudomonas aeruginosa: host defence in lung diseases. Respirology 15, 1037-1056

Cite this Article:

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Issue November 2018

Volume 7, November 2018


Table of Contents


Order Print Copy

World-wide Delivery is FREE

Share this Issue with Friends:


Submit your Paper