Potential Assessment of Leaf Ethanolic Extract Honje (Etlingera Hemisphaerica) in Regulating Glucose and Triglycerides on Mice (Mus Musculus)

Potential Assessment of Leaf Ethanolic Extract Honje (Etlingera Hemisphaerica) in Regulating Glucose and Triglycerides on Mice (Mus Musculus)

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Author(s): Aceng Ruyani, Agus Sundaryono, Zico Fakhrur Rozi, Dian Samitra, Efri Gresinta

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749 2060 70-76 Volume 3 - Jan 2014


This study was aimed to assess potential of leaf ethanolic extract Etlingera hemisphaerica in regulating glucose and triglyseride on mice (Mus musculus). METHODS: Two extract assesments were composed of five group (1 control, 3 dosages of extract, and 1 comparator drug), and each group were replicated by 5 of M. musculus. Pathological conditions were performed by giving 10 % sugar in drink for hyperglycemia, and a high fat diet for hypertriglyceride during 10 days. There were three dosages of the extract (0.13, 0.26, and 0.39) mg/g body weight (bw), and two comparator drugs (glibenclamid 0.52 mg/g bw as anti diabetic and simvastatin 0.052 mg/kg bw as anti hypertriglyceride). Both dosage extract and comparator drug were administrated by gavage on day 11, while the control group received only sesame oil solvent in the same manner. Blood samples were obtained from the tail to determine glucose and triglyceride levels before and after treatment. RESULTS: Treatment with 0.39 mg/gbw extracts decreased glucose from baseline (117.8 mg/dL) to the end (75.20 mg/dL) on day 13 as much as 36.16 % while glibenclamid decreased 38.00%. Triglyceride test revealed that 0.39 mg/gbw extract decreased from baseline (127.4 mg/dL) to the end (100.4 mg/dL) on day 13 as much as 21.19% which was equivalent to 21.20% caused by simvastatin. CONCLUSION: Leave ethanolic extract of E. hemisphaerica (0.39 mg/g bw) potentially decrease blood glucose (36.2 %) and triglyceride (21.19%) in M. musculus.


E.hemispaerica, ethanolic extract, M. musculus, glucose, triglyceride


  1. Artanti, D. 2008. Pengaruh Pemberian Jus Buah Pare (Momordica charantia) Terhadap Kadar Trigliserida Serum Tikus Wistar Jantan Yang Diberi Diet Tinggi Lemak
  2. Ayoola, GA., Coker, HAB., Adesegun, SA., Adepoju-Bello, AA., Obaweya, K., Ezennia, EC., and Atangbayila, TO. 2008. Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria. Trop J Pharm Res., 7(3): 1019-1024
  3. Azmi, MB., and Qureshi, SA. 2012. Methanolic Root Extract of Rauwolfia serpentina Benth Improves the Glycemic, Antiatherogenic, and Cardioprotective Indices in Alloxan-Induced Diabetic Mice. Adv Pharmacol Sci., 376429. doi: 10.1155/2012/376429
  4. Chan, EW., Lim, YY., and Tan , SP. 2011. Standardised herbal extract of chlorogenic acid from leaves of Etlingera elatior (Zingiberaceae). Pharmacognosy Res., 3(3):178-84
  5. Chiasson, JL., Josse, RG., Gomis, R., Hanefeld, M., Karasik, A., and Laakso, M. 2004. Acarbose for the prevention of type 2 diabetes, hypertension and cardiovascular disease in subjects with impaired glucose tolerance: facts and interpretations concerning the critical analysis of the STOP-NIDDM Trial data. Diabetologia 47: 969–975
  6. Fu, T., and Borensztajn, J. 2006. Simvastatin causes the formation of cholesterol-rich remnants in mice lacking apoE. Biochem Biophys Res Commun., 341(4):1172-6
  7. Ganugapati, J., Baldwa, A. and Lalani, S 2012. Molecular docking studies of banana flower flavonoids as insulin receptor tyrosine kinase activators as a cure for diabetes mellitus. Bioinformation., 8(5):216-20. doi: 10.6026/97320630008216
  8. Gross, M. 2004. Flavonoids and Cardiovascular Disease. Pharmaceutical Biology. 42: 21–35
  9. Hardhani, A. S. 2008. Pengaruh Pemberian Ekstrak Daun Salam (Eugenia polyantha) Terhadap Kadar Trigliserida Serum Tikus Jantan Galur Wistar Hiperlipidemia [Skripsi]. Semarang: Fakultas Kedokteran Universitas Diponegoro
  10. Hil, EF., De, HV., Beekmann, K., Keijer, J., Hollman, PCH., Ivonne, MC, Rietjens, M., Schothorst, EM 2011. Interference of flavonoids with enzymatic assays for the determination of free fatty acid and triglyceride levels. Anal Bioanal Chem. 402:1389–1392
  11. Hogan, Zhang.L, Zhou.K, Sun. S, Canning.C . 2010. Antioxidant rich grape pomace extract suppresses postprandial hyperglycemia in diabetic mice by specifically inhibiting alpha-glucosidase. Nutr Metab (Lond)., 7:71. doi: 10.1186/1743-7075-7-71.
  12. Jackie, T., Haleagrahara, N., and Chakravarthi, S. 2011. Antioxidant Effects of Etlingera elatior Flower Extract Against Lead Acetate-Induced Perturbations in Free Radical Scavenging Enzymes and Lipid Peroxidation in Rats. BMJ Reasearch Notes. 4(67):1-8
  13. Khomsan, A. 2008. Empat Serangkai Penggempur Kolesterol. http://www.indomedia.com. Akses 23 Februari 2012
  14. Lee YS, Cha BY, Saito K, Choi SS, Wang XX, Choi BK, Yonezawa T, Teruya T, Nagai K, and Woo JT. 2011. Effects of a Citrus depressa Hayata (shiikuwasa) extract on obesity in high-fat diet-induced obese mice. Phytomedicine. 15:648–654. [PubMed: 21216135]
  15. Li RW, Theriault AG, Au K, Douglas TD, Casaschi A, Kurowska EM, and Mukherjee R. 2006. Citrus polymethoxylated flavones improve lipid and glucose homeostasis and modulate adipocytokines in fructose-induced insulin resistant hamsters. Life Sci., 79:365–373. doi: 10.1016/j.lfs.2006.01.023. [PubMed: 16497336]
  16. Liu, Y., Sun, J., Rao, S., Su, Y., and Yang, Y. 2013. Antihyperglycemic, antihyperlipidemic and antioxidant activities of polysaccharides from Catathelasma ventricosum in streptozotocin-induced diabetic mice. Food Chem Toxicol., 57C:39-45
  17. Majid, A. 2007. Penyakit jantung Koroner: Patofisiologi, pencegahan dan pengobatan terkini. (Pidato pengukuhan jabatan guru besar) http://repository.usu.ac.id/ bitstream/ 123456789/705/1/08E00124.pdf Akses 17 April 2012
  18. Metwally, MAA., El-Gellal, AM, and El-Sawaisi, SM. 2009. Effects of Silymarin on Lipid Metabolism in Rats. World Applied Sciences Journal, 6 (12): 1634-1637
  19. Mulvihill, EE., Emma, A., Brian, G S, Dawn, ET, Cynthia, GS., Jane, YE, Janet M. Markle, Robert A. Hegele, and Murray, WH. 2009. Naringenin Prevents Dyslipidemia, Apolipoprotein B Overproduction, and Hyperinsulinemia in LDL Receptor–Null Mice With Diet-Induced Insulin Resistance. A Journal Of The American Diabetes Assocation. 58(10)
  20. Naufalin, R, Jenie, BSL., Kusnandar, F. Sudarwamto, M. Rukmini, H. 2005. Kajian Sifat Antimikroba Ekstrak Bunga Kecombrang Terhadap Berbagai Mikroba Patogen dan Perusak Pangan. Jurnal Teknologi dan Industri Pangan, 16 (2); 119-125
  21. Odo, RI., Asuzu, IU., and Aba, PE. 2012. The antidiabetic activities of the methanolic root bark extract of Afzelia africana in alloxan-induced diabetic mice. J Complement Integr Med., 9;9:Article 31. doi: 10.1515/1553-3840.1649
  22. Ruela, HS., Sabino, KC., Leal, IC., Landeira-Fernandez, AM., de Almeida, MR., Rocha, TS., and Kuster, RM. 2013. Hypoglycemic effect of Bumelia sartorum polyphenolic rich extracts. Nat Prod Commun., 8(2):207-10
  23. Ruyani, A., Karyadi, B., Kadir, A. Fitri, D., Tanjung, RY., Puspa, Y. 2011. Alteration of Ossification Rate on Fetal Humerus and Femur Swiss Webster Mice (Mus muculus) as the Teratogenic Effects of Dioscorea hispida Dennst. Jurnal Kedokteran dan Farmasi, Medika, 37, 596-603
  24. Ruyani, A., Sudarwati, S., Sutasurya, LA., Sumarsono, SH., Kim, D.J., and Chung, J.H. 2005. A teratoproteomics analysis: Heat shock protein 70 is up-regulated in mouse forelimb bud by methoxyacetic acid treatment. Birth Defects Research A Clinical and Molecular Teratology, 73 (7), 517-21
  25. Sang, H., Yuan, N., Yao, S., Li, F., Wang, J., Fang, Y., and Qin, S. 2012. Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in ApoE-deficient mice. Lipids Health Dis., 11:166
  26. Santoso, M., dan Setiawan, T. 2007. Penyakit Jantung Koroner. http://www.kalbe.co.id/files /147_05 penyakit jantung koroner.pdf/147_05 penyakit jantung koroner.html. Akses 21 November 2011
  27. Sopi, RB and Khan, MFH. 2013. Bronchodilatory effect of ethanolic extract of the leaves of Nyctanthes arbortristis. Pharmacognosy Res., 5(3): 169–172. doi: 10.4103/0974-8490.112422. PMCID: PMC3719257
  28. Steel, RGD, and Torrie, JH. 1981. Principles and procedures of statistics. Mc Graw-Hill International Book Company. Singapura
  29. Sunarso, E. 2011. Pengaruh Ekstrak Kulit Batang Mulitinga calbura Terhadap Kadar Trigliserida Darah Mus musculus Sebagai Sumber Belajar Kimia (LKS) di SMKN 1 Curup. [Tesis] Prodi Pascasarjana Pendidikan IPA, Universitas Bengkulu
  30. Wan, L., Chen, C., Xiao, Z., Wang, Y., Min, Q., Yue, Y., and Chen J. 2013. In vitro and in vivo anti-diabetic activity of Swertia kouitchensis extract. J Ethnopharmacol., pii: S0378-8741(13)00208-0
  31. X H., Xu, HE., and Ryan, D. 2009. A study of the comparative effects of hawthorn fruit compound and simvastatin on lowering blood lipid levels. Am J Chin Med., 37(5):903-8

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