Identification and Investigation of Phenotypic and Genotypic Characteristics of Flavobacterıum Psychrophılum ın Fry Raınbow Trouts (Oncorhynchus Mykıss) in Some Trout

Identification and Investigation of Phenotypic and Genotypic Characteristics of Flavobacterıum Psychrophılum ın Fry Raınbow Trouts (Oncorhynchus Mykıss) in Some Trout

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Author(s)

Author(s): Mikail ÖZCAN, Mustafa SARIEYYÜPOĞLU

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445 1039 24-34 Volume 3 - Mar 2014

Abstract

In this study, it was aimed to identify and investigate phenotypic and genotypic charecteristics of Flavobacterium psychrophilum in four rainbow trout farms in Keban district of Elazığ province. Two different primers were used to confirm 160 Flavobacterium psychrophilum with PCR after determined according to the phenotype and genotype characteristics. Subsequently, these strains were confirmed as Flavobacterium psychrophilum with DNA. Gene typing was performed with PFGE technique on strains confirmed with PCR. As a result of this, it was decided that strains obtained from four different fish farms wasclose relative with reference Flavobacterium psychrophilum NCIMB 1947T.

Keywords

Flavobacterium psychrophilum, Rainbow trout, Oncorhynchus mykiss, Polymerase Chain Reaction, PCR, Pulsed Field Gel Electrophoresis, PFGE

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