Identification and Investigation of Phenotypic and Genotypic Characteristics of Flavobacterıum Psychrophılum ın Fry Raınbow Trouts (Oncorhynchus Mykıss) in Some Trout

Identification and Investigation of Phenotypic and Genotypic Characteristics of Flavobacterıum Psychrophılum ın Fry Raınbow Trouts (Oncorhynchus Mykıss) in Some Trout

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Author(s)

Author(s): Mikail ÖZCAN, Mustafa SARIEYYÜPOĞLU

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448 1060 24-34 Volume 3 - Mar 2014

Abstract

In this study, it was aimed to identify and investigate phenotypic and genotypic charecteristics of Flavobacterium psychrophilum in four rainbow trout farms in Keban district of Elazığ province. Two different primers were used to confirm 160 Flavobacterium psychrophilum with PCR after determined according to the phenotype and genotype characteristics. Subsequently, these strains were confirmed as Flavobacterium psychrophilum with DNA. Gene typing was performed with PFGE technique on strains confirmed with PCR. As a result of this, it was decided that strains obtained from four different fish farms wasclose relative with reference Flavobacterium psychrophilum NCIMB 1947T.

Keywords

Flavobacterium psychrophilum, Rainbow trout, Oncorhynchus mykiss, Polymerase Chain Reaction, PCR, Pulsed Field Gel Electrophoresis, PFGE

References

  1. Arai, H., Morita, Y., Izumi, S., Katagiri, T., Kimura, H., 2007. Molecular typing by pulsed-field gel electrophoresis of Flavobacterium psychrophilum isolates derived from Japanese fish. J Fish Dis.:30(6):345–55
  2. Arda, M., 2000. Temel Mikrobiyoloji; "Genişletilmiş İkinci Baskı.. Medisan Yayın Serisi no 46. Ankara"
  3. Arda, M., Seçer, S., Sarıeyyüpoğlu, M., 2005. Balık Hastalıkları Medisan Yayın serisi: 61, II. Baskı Ankara 230s
  4. Austin, B and Austin, D.A., 1987. Bacterial Fish Pathogens: Disease in Farmed and Wild Fish. Ellis Horwood Ltd. Chichester, 363s
  5. Austin, B., 1992. The recovery of Cytophaga psychrophila from Two Cases of Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fry Syndrome in the U.K. Bull. Eur. Assoc. Fish Pathol. 12: 207–208
  6. Austin, B., and Austin, D. A. 1999. Fish Pathogens Disease in Farmed and Wild Fish, 3rd ed. (revised), Praxis Publ., Chiester, UK
  7. Balta, F., 1997. Kültürü yapılan alabalıklarda (Oncorhynhus mykiss) görülen Flexibacter psychrophila enfeksiyonu. IX. Ulusal Su Ürünleri Sempozyumu Eğirdir/İsparta, 641–648
  8. Bej, A.K., Mahbubani M.H., Atlas, R.M., 1991. Amplification of Nucleic Acids by Polymerase Chain Reaction (PZR) and Other Method and Their Amplications. Critical Reviews in Biochemistry and molecular Biology, 26, 301–334
  9. Bernardet, J.F. and Keroualt, B., 1989. Phenotypic and Genomic Studies of ‘‘Cytophaga psychrophila’’, Isolated from Diseased Rainbow Trout (Oncorhynhus mykiss) in France. Appl. Environ. Microbial. Vol. 55, No: 7, 1796–1800
  10. Bernardet, J.F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. and Vandamme, P., 1996. Cutting a Gordian Knot: Emended Classification and Description of the Genus Flavobacterium, Emended Description of the Family Flavobacteriaceae, and Proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978) Internatıonal Journal Of Systematıc Bacterıology, Jan. 128–148
  11. Bowser, P.R., 1999. “Diseases of Fish”, PhD, Aquatic Animal Health Program, Department of Microbiology and Immunology, College of Veterinary Medicine Cornell University, Ithaca, New York, 26 p
  12. Bruno, D.W., 1992. “Cytophaga psychrophila, (Flexibacter psychrophilus)(Borg), histophathology associated with mortalities among farmed rainbow trout (Oncorhynchus mykiss) (Walbaum) in the UK ”, Bull. Eur. Ass. Fish Pathol., 12(6):215-216
  13. Buckley, R., Clough, E., Warken, W., Wıld, C., 1998. Coliform Bacteria in Streambed Sediments in a Subtropical Rainforest Conservation Reserve. Wat.Res., 32(6), 1852-1856
  14. Bustos, P., Calbuyahue, A., Maontaña, J., Opazo, B., Entrala, P. and Solervicens, R., 1995. First isolation of Flavobacterium psychrophilum as causative agent of rainbow trout fry syndrome (RTFS) in Chile. Bull. Eur. Assoc. Fish Pathol. 15: 162–164
  15. CDC, 2010. One-Day (24-28 h) Standardized Laboratory Protocol for Molecular Subtyping of Escherichia coli O157:H7, non-typhoidal Salmonella Serotypes, and Shigella sonnei by Pulsed Field Gel Electrophoresis (PFGE). EriÅŸim: (http://www.cdc.gov/PULSENET/protocols/ecoli_salmonella_shigella_protocols.pdf). EriÅŸim tarihi: 28.06.2010
  16. Cengizler, İ., 2000. Balık Hastalıkları Ders Kitabı. Çukurova Ünivesitesi Su Ürünleri Fakültesi Yayınları No: 7, 136s
  17. Chen, Y.C., Davis, M.A., Lapatra, S.E., Cain, K.D., Snekvik, K.R., Call, D.R., 2008. Genetic diversity of Flavobacterium psychrophilum recovered from commercially raised rainbow trout, Oncorhynchus mykiss (Walbaum), and spawning coho salmon, O. kisutch (Walbaum) J Fish Dis. :31(10):765-73
  18. Cipriano, R.C. and Holt R.A., 2005. Flavobacterium psychrophilum, Cause of Bacterial Cold- Water Disease and Rainbow Trout Fry Syndrome. Fish Disease Leaflet No:86. United States Dept. of the Interior. U.S. Geological Service, National Fish Health Research Laboratory, Kearneysville, WV. 44s
  19. Crump, E.M., Perry, M.B., Clouthıer, S.C., Kay, W.W., 2001. Antigenic Characterization of the Fish Pathogen Flavobacterium psychrophilum, Applıed and environmental microbiology, 67, 750-759
  20. Dalsgaard, I. and Madsen, L., 2000. Bacterial pathogens in rainbow trout Oncorhynchus mykiss (Walbaum), reared at Danish freshwater farms. J. Fish Dis. 23: 199–209
  21. Daskalov, H., Austin, D.A., Austin B., 1999. An improved growth medium for Flavobacterium psychrophilum. Letters in Applied Microbiology 28: 297–299
  22. Del Cerro, A., Márquez I., Prieto J.M., 2010. Genetic diversity and antimicrobial resistance of Flavobacterium psychrophilum isolated from cultured rainbow trout, Onchorynchus mykiss (Walbaum), in Spain. J Fish Dis. :33(4):285–91
  23. Derbentli Ş., 2002. Hastane infeksiyonlarının epidemiyolojisinde moleküler yöntemlerin yeri. Ağaçfidan A, Badur S, Türkoğlu S (Editörler). İnfeksiyon hastalıklarının laboratuar tanısında moleküler yöntemler’inde. İstanbul: Türk mikrobiyoloji cemiyeti yayın; 6–13
  24. Didinen, B.I., Diler, Ö., Ekici, S., Altun, S., 2005. Flavobacterium psychrophilum izolatlarının teşhisinde API ZYM kullanımı ve ATB VET ile antimikrobiyal duyarlılığın belirlenmesi, Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 1 (2): 64-70
  25. Diler, Ö., Altun, S., Çalıkuşu, F., Diler, A., 2000. Gökkuşağı alabalığı (Oncorhynchus mykiss)’nin yaşadığı ortam ile ilişkili kalitatif ve kantitatif bakteriyel florası üzerine bir araştırma. Turk J. Vet. Anim. Sci. 24: 251–259
  26. Diler, Ö., Altun, S., Işıklı, B.I., 2003. Kültürü yapılan gökkuşağı alabalığı (Onchorhynchus mykiss)’ndan izole edilen Flavobacteriun psychrophilum’un fenotipik karakterleri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 7.1, 1–8
  27. Domig, J.K., Mayer, HK., Kneifel, W., 2003. Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp.2. Pheno- and genotypic criteria. International Journal of Food Microbiology.; 88 :165–188
  28. Ekman, E., 2003. Natural and experimental Infections with Flavobacterium psychrophilum in salmonid fish. Swedish University of agricultural sciences, Doctoral thesis, 47s
  29. Ekman, E., Börjeson, H., Johansson, N., 1993. Flavobacterium psychrophilum in Baltic salmon Salmo salar brood fish and their offspring. Dis Aquat Org, 37, 159-163
  30. Elsayed, E.E., Eissa, A.E., Faisal, M., 2006. Isolation of Flavobacterium psychrophilum from sea lamprey, Petromyzon marinus L., with skin lesion in Lake Ontairo, Journal of Fish Diseases, 29: 629-632
  31. Gonzalez, C.J., Lopez-Diaz, T.M., Garcia-Lopez, M.L., Prieto, M., Otero, M., 1999. Bacterial microflora of wild brown trout (Salmo trutta), wild pike (Esox lucius) and aquacultured rainbow trout (Oncorhynchus mykiss), Journal of Food Protection, 62(11): 1270-1277
  32. Gonzalez, C.J., Santos, J.A., Garcia-Lopez, M.L., Gonzalez, N., Otero, A., 2001. Mesophilic aeromonads in wild and aquacultured freshwater fish, Journal of Food Protection, 64(5): 687-691
  33. Hatha, M., Vivekanandhan, A.A., Joice, G.J., Christol, J.J., 2005. Antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish, International Journal of Food Microbiology, 98(2):131-134
  34. Holt, R.A., 1987. Cytophag, Psychrophil, the Causative Agent of Bacterial Cold-Water Disease in Salmonid Fish Oregon State University A Thesis 182 p
  35. Holt, R.A., Rohovec, J.S. and Fryer, J.L., 1993. “ Bacterial cold-water disease ”, In: “Bacterial Diseases of Fish ”, Inglis, V., Roberts, R. and Bromage, N. R. (ed.), Blackwell Scientific Publications, Oxford., 312p
  36. İspir, Ü., Şeker, E., Sağlam, N., Dörücü, M., 2004. Doğu Anadolu bölgesinde bazı gökkuşağı alabalığı (Oncorhynchus mykiss) işletmelerinde görülen Flavobacterium psychrophilum enfeksiyonunun araştırılması, Fırat Üniversitesi Fen ve Mühendislik Bilimler dergisi, 16 (4), 718-724
  37. Korun, J. ve Timur, G., 2001. Gökkuşağı Alabalıklarında (Onchorhynchus mykiss) fry mortalite sendromu (FMS) üzerinde bir çalışma İstanbul Üniversitesi su ürünleri dergisi 12:15-30
  38. Kubilay A., Altun, S., Savaş S., 2009b. A study on aerobic bacterial flora during incubation of rainbow trout (Oncorhynchus mykiss, Walbaum 1792) eggs in hatchery. Journal of FisheriesSciences. 3(1) 5–9
  39. Kubilay, A., Altun, S., Didinen, B.I., Ekici, S., Diler, Ö., 2009a. Gökkuşağı Alabalığı (Oncorhyncus mykiss) İşletmelerinde Flavobacterium psychrophilum İzalosyonu Kafkas Univ. Vet. Fak. Derg. 15 (5): 709–715
  40. Kumagai, A., Nakayasu, C., Oseko, N., 2004. Effect of Tobramycin Supplementation to Medium on Isolation of Flavobacterium psychrophilum from Ayu Plecoglossus altivelis Japan Science and Technology Agency 39;2; 75-78
  41. Lin, C.K., and Tsen H.Y., 1996. Use of two 16S DNA Targeted Oligonücleotides as PZR Primers for the Specific Detection of Salmonella in Foods. Journal of Applied Bacteriology, 80: 659–666
  42. Lorenzen, E., 1994. Studies on Flexibacter psychrophilus in relation to rainbow trout fry syndrome (RTFS), National Veterinary Laboratory, Royal Veterinary and Agricultural University, Copenhagen
  43. Lorenzen, E., Dalsgaard, I., Bernardet, J.F., 1997. Characterization of isolates of Flavobacterium psychrophilum associated with coldwater disease or rainbow trout fry syndrome I: phenotypic and genomic studies, Diseases of Aquatic Organisms, 31: 197-208
  44. Lorenzen, E., Dalsgaard, I., From, J., Hansen, F. M., Horlyck, V., Korsholm, H., Mellergaard, S. and Olesen, N.J., 1991. Preliminary investigations of fry mortality syndrome in rainbow trout. Bull. Eur. Assoc. Fish Pathol. 11: 77–79
  45. Lumsden, J.S., Ostland, V.E. and Ferguson, H.W., 1996. Necrotic Myositis in Cage Cultured Rainbow Trout, Oncorhynhus mykiss (Walbaum), Caused by Flexibacter psychrophilus. Journal of fish diseases 19; 113–119
  46. Madetoja, J. and Wiklund, T., 2002. Detection of the fish pathogen Flavobacterium psychrophilum in water from fish farms. System. Appl. Microbiol. 25: 259–266
  47. Madetoja, J., Dalsgaard, I., Wiklund, T., 2002. Occurrence of Flavobacterium psychrophilum in fish-farming environments. Dis Aqua Org, 52, 109-118
  48. Madetoja, J., Hänninen, M-N., Hirvelä-Koski, V., Dalsgaard, I. and Wiklund, T., 2001. Phenotypic and genotypic characterization of Flavobacterium psychrophilum from Finnish fish farms. J. Fish Dis. 24:469–479
  49. Madsen, L and Dalsgaard, I., 2008. Water recirculation and good management potential methods to avoid disease outbreaks with Flavobacterium psychrophilum. J Fish Dis, 31, 799-810
  50. Maslow, J.N., Slutsky, A.M. and Arbeit, R.D., 1993. Application of PFGE to molecular epidemiology. P: 563–572. Diagnostic Molecular Microbiology: Principles and Applications. ASM Washington D.C
  51. Mullis, K.B., 1990. The Unusual Origin of the Polymerase Chain Reaction. Sci. Am. 262 (4) : 56 – 65
  52. Nematollahi, A ., Decostere, A ., Pasmans, F. and Haesebrouck, F., 2003. Flavobacterium psychrophilum infections in salmonid fish. Journal of Fish Diseases, 26: 563–574
  53. Ostland, V.E., McGrogan, D.G., Ferguson, H.W., 1997. Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus, Journal of Fish Diseases, 20: 443-451
  54. Pacha, 1968. Characteristics of Cytophaga psychrophila (Borg) Isolated During Outbreaks of Bacterial Cold-Water Disease, Applıed Mıcrobıology, p. 97-101
  55. Rangdale, R.E., Richards R.E., Alderman, D.J., 1996. Isolation of Cytophaga psychrophila, causal agent of rainbow trout fry syndrome (RTFS) from reproductive fluids and egg surfaces of rainbow trout (Oncorhynchus mykiss). Bull Eur Ass Fish Pathol 16, 63-67
  56. Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B. and Erlich, H.A., 1988. Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase. Science; 239, 487–491
  57. Sander, J.E., Fryer J.L., 1988. Bacteria of Fish in "Methods in Aquatic Bacteriology" (Ed by AUSTIN), John wiley & Sons ltd., Cichester, New York, Brisbane, Toronto, Singapore
  58. Sarıeyyüpoğlu, M., 1984. Gökuşağı Alabalıklarında (S. gairdneri) Mide-Bağırsak Bakteriyel Florasının Aerobik Yönden İncelenmesi. Doğa Bilim Dergisi, Seri: D 1 Cilt: 8, Sayı: 3, S. 281-287
  59. Schmidt, A.S., Bruun, M.S., Dalsgaard, I., Pedersen, K. and Larsen, J.L., 2000. Occurrence of antimicrobial resistance in fish-pathogenic and environmental bacteria associated with four Danish rainbow trout farms. Applied and Environmental Microbiology 66, 4908–4915
  60. Schmidtke, L.M., Carson, J., 1995. Characteristics of Flexibacter psychrophilus isolated from Atlantic salmon in Australia, Diseases of Aquatic Organisms, 21:157-161
  61. Skrodenyte-Arbaciauskiene, V., Sruoga, A., Butkauskas, D., 2006. Assessment of microbial diversity in the river trout, Salmo trutta fario L., intestinal tract identified by partial 16SrRNA gene sequence Analysis. Fisheries Science, 72(3): 597–602
  62. Starliper, C., Marcquenski S., Noyes A., 2007. Development of an improved medium for primary isolation of Flavobacterium psychrophilum, cause of bacterial coldwater disease
  63. Suzuki, K., Arai, H., Kuge, T., Katagiri, T., Ä°zumi S., 2008. Reliability of PCR Methods fort he Detection Flavobacterium psychrophilum. Fish Patology, 43 (3), 124-127
  64. Taylor, G.R., 1993. Polymerase Chain Reaction: Basic Principles and Automation.In PZR: A Pratical Approach, Eds:McPherson, M.J., Quirke, P. and Taylor, G.R.Oxford University Press,Oxford, 1-14
  65. Toranzo, A.E. and Barja, L.J., 1993. “Fry Mortality Syndrome (FMS) In Spain. Isolation of The Causative Bacterium Flexibacter psychrophilus”, Bull. Eur. Ass. Fish Pathol., 13(1):30-32
  66. 66. Toshio, N., Yuichi, I., Hajime, A., Takahiro N., Shotaro, I., 2006. A Newly Designed Culture Medium for Flavobacterium psychrophilum Japan Science and Technology Agency 28;.33-37
  67. Van Belkum, A., Tassios, P.T., Dijkshoorn, L., Haeggman, S., Cookson, B., Fry, NK., Fussing, V., Gren, J., Feil, E., Gerner-Smidt, P., Brisse, S., Struelens, M., 2007. Guidelines for the validation and applicationof typing methods for use in bacterial epidemiology. Clinical Microbiology and Infectious Diseases.; 13: 1–46
  68. Vastos, I.N., Thompson, K.D. and Adams, A., 2003. Starvation of Flavobacterium psychrophilum in broth, stream water and distilled water. Dis. Aquat. Org. 56: 115–126
  69. Wiklund, T., Kaas, K., Lönnström, L., Dalsgaard, I., 1994. Isolation of Cytophaga psychorphila (Flexibacter psychorphilus) from wild and farmed rainbow trout (Oncorhynchus mykiss) in Finland, Bulletin of the European Association of Fish Pathologists, 14 (2): 44-46
  70. Yıldırım, S., 2007. Mersin Çağlarca Köyündeki Gökkuşağı Alabalığı (Oncorhynchus mykiss,Walbaum, 1792) Kuluçkahanelerinde Flavobacterıum spp.’nin Araştırılması. Mersin Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi 79s
  71. Barrett T.J., Lior H., Green J.H., Khakhria R., Wells J.G., Bell B.P., Greene K.D., Lewis J. & Griffin P.M. (1994) Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel electrophoresis and phage typing. Journal of Clinical Microbiology 32,3013–3017
  72. Izumiya H., Terajima J., Wada A., Inagaki Y., Itoh K., Tamura K. & Watanabe H. (1997) Molecular typing of enterohemorrhagic Escherichia coli O157:H7 isolates in Japan by using pulsed-field gel electrophoresis. Journal of Clinical Microbiology 35, 1675–1680

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