Inhibition of Human Parainfluenza Virus Type 2 Growth in Vitro by Catechin is caused by the Inhibition of Genome and mRNA Syntheses and by the Disruption of Cytoskeleton, and that by Tannic Acid is Mainly Caused by Genome Synthesis Inhibition and the Disruption of Cytoskeleton

Inhibition of Human Parainfluenza Virus Type 2 Growth in Vitro by Catechin is caused by the Inhibition of Genome and mRNA Syntheses and by the Disruption of Cytoskeleton, and that by Tannic Acid is Mainly Caused by Genome Synthesis Inhibition and the Disruption of Cytoskeleton

Loading document ...
Page
of
Loading page ...

Author(s)

Author(s): Hiroshi Komada, Jun Uematsu, Sahoko Kihira, Mika Uchida, Yurika Sakakura, Sayuri Deguchi, Kae Sakai, Hidetaka Yamamoto, Mitsuo Kawano, Masato Tsurudome, Myles O’Brien

Download Full PDF Read Complete Article

353 809 47-55 Volume 3 - Dec 2014

Abstract

The antiviral activities of catechin mixture (catechin) and tannic acid against human parainfluenza virus type 2 (hPIV-2) were investigated in vitro. Catechin and tannic acid both inhibited cell fusion induced by hPIV-2 in LLCMK2 cells. However, high concentrations of them caused cell toxicity. Both catechin and tannic acid reduced the number of viruses released from the cells. Real time PCR showed that catechin almost completely inhibited virus genome RNA synthesis, and tannic acid largely inhibited it. Virus nucleoprotein (NP), fusion (F) and hemaggulutinin-neuraminidase (HN) gene syntheses were largely inhibited by catechin, and mRNA syntheses of these proteins were partly inhibited by catechin. However, tannic acid did not cause inhibition. An indirect immunofluorescence study showed that catechin partly inhibited virus NP, F and HN protein syntheses, but tannic acid did not inhibit either virus NP, F or HN protein syntheses. Using a recombinant green fluorescence protein (GFP)-expressing hPIV-2 without matrix protein (rhPIV-2∆MGFP), it was found that virus entry into the cells was not inhibited by catechin or tannic acid, and that spreading of virus to the adjacent cells was not blocked by them. Catechin and tannic acid disrupted both actin microfilaments and microtubules. These results indicated that the inhibitory effect of catechin was caused by the inhibitions of both viral genome RNA and mRNA syntheses, and by the disruption of actin microfilaments and microtubules. However, inhibition by tannic acid was mainly caused by vial genome synthesis inhibition and the disruption of cytoskeleton.

Keywords

human parainfluenza virus type 2, catechin mixture, tannic acid, a recombinant green fluorescence protein expressing hPIV-2 without matrix protein

References

  1. Lamb R.A., Parks G.P. (2007) Paramyxoviridae: The viruses and their Replication. In: Kniep D.M., Howley P.M., eds. Fields Virology, 5th edn. Philadelphia: Lippincott Williams and Wilkins, pp1449-1496
  2. Yuasa T., Bando H., Kawano M., Tsurudome M., Nishio M., Kondo K., Komada H., Ito Y. (1990) Sequence analysis of the 3’ genome end and NP gene of human parainfluenza type 2 virus: sequence variation of the gene-starting signal and the conserved 3’ end. Virology 179: 777-784
  3. Ohgimoto S., Bando H., Kawano M., Okamoto K., Kondo K., Tsurudome M., Nishio M., Ito Y. (1990) Sequence analysis of P gene of human parainfluenza type 2 virus: P and cystein-rich proteins are translated by two mRNAs that differ by two non-templated G residues. Virology 177: 116-123
  4. Kawano M., Bando H., Ohgimoto S., Okamoto K., Kondo K., Tsurudome M., Nishio M., Ito Y. (1990) Complete nucleotide sequence of the matrix gene of human parainfluenza type 2 virus and expression of the M protein in bacteria. Virology 179: 857-861
  5. Kawano M., Bando H., Ohgimoto S., Okamoto K., Kondo K., Tsurudome M., Nishio M., Ito Y. (1990) Sequence of the fusion protein gene of human parainfluenza type 2 virus and its 3’ intergenic region: lack of small hydrophobic (SH) gene. Virology 178: 289-292
  6. Kawano M., Bando H., Yuasa T., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. (1990) Sequence determination of the hemagglutinin-neuraminidase (HN) gene of human parainfluenza type 2 virus and the construction of a phylogenetic tree for HN proteins of all the paramyxoviruses that are infectious to humans. Virology 174: 308-313
  7. Kawano M., Okamoto K., Bando H., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. (1991) Characterizations of the human parainfluenza type 2 virus gene encoding the L protein and the intergenic sequences. Nucleic Acids Res 19: 2739-2246
  8. Tsurudome M., Nishio M., Komada H., Bando H., Ito Y. (1989) Extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramxoviruses revealed by monoclonal antibodies. Virology 171: 38-48
  9. Kawano M., Kaito M., Kozuka Y., Komada H., Noda N., Namba K., Tsurudome M., Ito M., Nishio M., Ito Y. (2001) Recovery of infectious human parainfluenza type 2 virus from cDNA clones and properties of the defective virus without V-specific cysteine-rich domain. Virology 284: 99-112
  10. Steinmann J., Buer J., Pietschmann T., Steinmann E. (2013) Anti-infective properties of epigallocatechin-3-gallate (EGCG), a component of green tea. British J Pharmacol 168: 1059-1073
  11. Ueda K., Kawabata R., Irie T., Nakai Y., Tohya Y., Sakaguchi T. (2013) Inactivation of pathogenic viruses by plant-derived tannins: Strong effects of extracts from persimmon (Diospyros kaki) on a broad range of viruses. PLoS ONE 8: e55343
  12. Uematsu J., Koyama A., Takano S., Ura Y., Tanemura M., Kihira S., Yamamoto H., Kawano M., Tsurudome M., O’Brien M., Komada H. (2012) Legume lectins inhibit human parainfluenza virus type 2 infection by interfering with the entry. Viruses 4: 1104-1115
  13. De B.P., Banerjee A.K. (1999) Involvement of actin microfilaments in the transcription/replication of human parainfluenza virus type 3: possible role of actin in other viruses. Microsc Res Teck 47: 114-123
  14. Moyer S.A., Baker S.C., Lessard J.L. (1986) Tubulin: a factor necessary for the synthesis of both Sendai virus and vesicular stomatitis virus RNAs. Proc Natl Acad Sci USA 83: 5405-5409
  15. Kitagawa H., Kawano M., Yamanaka K., Kakeda M., Tsuda K., Inada H., Yoneda M., Sakaguchi T., Nigi A., Nishimura K., Komada H., Tsurudome M., Yasutomi Y., Nosaka T., Mizutani H. (2013) Intranasally administered antigen 85B gene vaccine in none-replacing human parainfluenza type 2 virus vector ameolirates mouse atopic dermatitis. PLoS ONE 8: e66614
  16. Buchholz U.J., Frink S., Conzelmann K.K. (1999) Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 73: 251-259
  17. Niwa H., Yamamura K., Miyazaki J. (1991) Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108: 193-199
  18. Weber J.M., Ruzindana-Umunyana A., Imbeault L., Sircar S. (2003) Inhibition of adenovirus infection and adenain by green tea catechins. Antiviral Res 58: 167-173
  19. Chang L.K., Wei T.T., Chiu Y.F., Tung C.P., Chuang J.Y., Hung S.K., Li C., Liu S.T. (2003) Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate. Biochem Biophys Res Commun 301: 1062-1068
  20. Song J.M., Lee K.H., Seong B.L. (2005) Antiviral effect of catechins in green tea on influenza virus. Antiviral Res 68: 66-74
  21. Kuzuhara T., Iwai Y., Takahashi H., Hatakeyama D., Echigo N. (2009) Green tea catechins inhibit the endnuclease activity of influenza A virus RNA polymerase. PLoS Curr 1: RRN1052
  22. Xu J., Wang J., Deng F., Hu Z., Wang H. (2008) Green tea extract and its major component epigallocatechin gallate inhibits hepatitis B virus in vitro. Antiviral Res 78: 242-249
  23. Ciesek S., Von Hahn T., Colpitts C.C., Schang L.M., Friesland M., Steinmann J. (2011) The green tea polyphenol, epigallocatechin-3-gallate, inhibits hepatitis C virus entry. Hepatology 54: 1947-1955
  24. Lin Y.T., Wu Y.H., Tseng C.K., Lin C.K., Chen W.C., Hsu Y.C., Lee J.C. (2013) Green tea phenolic epicatechins inhibit hepatitis C virus replication via cycloxygenase-2 and attenuate virus-induced inflammation. PLoS ONE 8: e54466
  25. Fassina G., Buffa A., Benelli R., Varnier O.E., Nooman D.M., Albini A. (2002) Polyphenolic antioxidant (-)-epigallocatechin-3-gallate from green tea as a candidate anti-HIV agent. Aids 16: 939-941
  26. Yamaguchi K., Honda M., Ikigai H., Hara Y., Shimamura T. (2002) Inhibitory effects of (-)-epigallocatechin gallate on the life cycle of human immunodeficiency virus type 1 (HIV-1). Antiviral Res 53: 19-34
  27. Williamson M.P., McCormick T.G., Nance C.L., Shearer W.T. (2006) Epigallocatechin gallate, the main polyphenol in green tea, binds to the T-cell receptor, CD4: potential for HIV-1 therapy. J Allergy Clin Immunol 118: 1369-1374
  28. Jiang F., Chen W., Yi K., Wu Z., Si Y., Han W., Zhao Y. (2010) The evaluation of catechins that contain a galloyl moiety as potential HIV-1 integrase inhibitors. Clin Immunol 137: 347-356
  29. Jsaacs C.E., Xu W., Merz G., Hillier S., Rohan L., Wen G.Y. (2011) Digallate dimers of (-)-epigallocatechin gallate inactivate herpes simplex virus. Antimicrob Agents Chemother 55: 5646-5653
  30. Ho H.Y., Cheng M.L., Weng S.F., Leu Y.L., Chiu D.T. (2009) Antiviral effect of epigallocatechin gallate on enterovirus 71. J Agric Food Chem 57: 6140-6147
  31. Zhang X.F., Dai Y.C., Zhong W., Tan M., Lv Z.P., Zhou Y.C., Jiang X. (2012) Tannic acid inhibited norovirus binding to HBGA receptors, a study of 50 Chinese medicinal herbs. Bioorg Med Chem 20: 1616-1623

Cite this Article:

  • BibTex
  • RIS
  • APA
  • Harvard
  • IEEE
  • MLA
  • Vancouver
  • Chicago

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

Search Articles

Issue June 2019

Volume 8, June 2019


Table of Contents


Order Print Copy

World-wide Delivery is FREE

Share this Issue with Friends:


Submit your Paper