Arbuscular Mycorrhizal Fungi in Alleviation of Drought Stress on Grain Yield and Yield Components of Mungbean (Vigna Radiata L.) Plants

Arbuscular Mycorrhizal Fungi in Alleviation of Drought Stress on Grain Yield and Yield Components of Mungbean (Vigna Radiata L.) Plants

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Author(s): Yagoob Habibzadeh

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DOI: 10.18483/ijSci.651 553 1325 34-40 Volume 4 - Mar 2015


To investigate the effect of mycorrhizal fungi on reduction of drought stress on related grain yield and yield components of mungbean plants, a pot culture was conducted based on Randomized Completely Design with three replications in Urmia University in 2009. The experiment with four irrigation regimes (25, 50, 75 and 100 mm of evaporation from a class A pan) were assigned at the first factor and two mycorrhiza species; Glomus mosseae, Glomus intraradices and a non-inoculated treatment at the second factor. Results showed that in both mycorrhizae species significantly (P<0.05) increased the grain yield, so Glomus intraradices (4.29 g/plant) and Glomus mosseae (4.31 g/plant) had the highest grain yield. Non inoculated treatment had the lowest (2.64 g/plant) grain yield. The maximum (5.14 g/plant) and minimum (1.97 g/plant) grain yield achieved in irrigation after 25 and 100 mm evaporation from pan, respectively. With increasing water deficit stress decreased relative water content, pod length, seeds/pod, pods/plant and seeds/plant. Mycorrhizae colonization (r=0.72**), relative water content (r = 0.76**), pod length (r = 0.90**), seeds/pod (r = 0.74**), pods/plant (r = 0.71**) and Seeds/plant (r = 0.86**) had the positive correlation coefficients with grain yield. Also, results showed that mycorrhizae species affected grain yield of mungbean plants through their effect on pod length, seeds/pod, pods/plant and seeds/plant under well-watered and drought stress conditions.


Colonization, Drought stress, Grain yield, Mungbean, Mycorrhiza, Yield components


  1. Abbaspour, H., Saeid-Sar, S., Afshari, H., and Abdel-Wahhab, M.A. 2012. Tolerance of mycorrhiza infected Pistachio (Pistacia vera L.) seedlings to drought stress under glasshouse conditions. J. Plant Physiol. 169:704–709.
  2. Abd-Alla, M.H., Omar, S.A., and Karanxha, S. 2000. The impact of pesticides on arbuscular mycorrhizal and nitrogen-fixing symbiosis in legumes. Appl. Soil Ecol. 14: 191-200.
  3. Al-Karaki, G.N., and Al-Raddad, A. 1997. Effects of arbuscular mycorrhizal fungi and drought stress on growth and nutrient uptake of two wheat genotypes differing in drought resistance. Mycorrhiza 7:83–88.
  4. Al-Karaki, G.N., and Clark, R.B. 1998. Growth, mineral acquisition, and water use by mycorrhizal wheat grown under water stress. J. Plant Nutr. 21:263–276.
  5. Al-Karaki, G.N., McMichael, B. and Zak J. 2004. Field response of wheat to arbuscular mycorrhizal fungi and drought stress. Mycorrhiza 14:263–269.
  6. Auge, R.M. 2001. Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza, 11(1): 3-42.
  7. Auge, R.M. 2004. Arbuscular mycorrhizae and soil/plant water relations. Canadian J. Soil Sci. 84: 373-381.
  8. Baslam, M., and Goicoechea, N. 2012. Water deficit improved the capacity of arbuscular mycorrhizal fungi for inducing the accumulation of antioxidant compounds in lettuce leaves. Mycorrhiza 22:347–359.
  9. Benami, A., and Ofen, A. 1984. Irrigation engineering-Sprinkler, trickle and surface irrigation: Principles, design and agricultural practices. Irrig. Eng. Sci. Publ., Haifa, Israel.
  10. Bray, E.A. 1997. plant responses to water deficit. Trends in Plant Sci. 2: 48-5.
  11. Caravaca, F., Diaz, E., Barea, J.M., Azcón-Aguilar, C., and Roldan, A. 2003. Photo-synthetic and transpiration rates of Olea europaea subsp. sylvestris and Rhamnus lycioides as aff ected by water defi cit and mycorrhiza. Biol. Plant 46:637–639.
  12. Demir‚ S. 2004. Influence of arbuscular mycorrhiza on some physiological‚ growth parameters of pepper. Turkish J. Biol. 28: 85-90.
  13. Duan, X., Neuman, D.S., J.M. Reiber, J.M., Geen, C.D., Saxton, A.M., and Auge, R.M. 1996. Mycorrhizal influence on hydraulic and hormonal factors implicated in control of stomatal conductance during drought. J. Exp. Bot. 47:1541–1550.
  14. Faber, B.A., Zasoski, R.J., Burau, R.G., and Uriu, K, 1990. Zinc uptake by corn as affected by vesicular-arbuscular mycorrhizae. Plant Soil 129:121–130.
  15. Faisal, E.A., Samia, O.Y., Elsiddig, A.E.E. 2000. Effects of mycorrhizal inoculation and phosphorus application on the nodulation, mycorrhizal infection and yield components of Faba Bean grown under two different watering regimes. University of Khartoum J. Agri. Sci. 8(2): 107-116.
  16. Hardie, K. 1985. The effect of removal of extraradical hyphae on water uptake by VAM plants. New Phytol. 101: 677–684.
  17. Habibzadeh, Y., Pirzad, A., Zardashti. M.R., Jalilian, J., and Eini, O. 2013. Effects of Arbuscular Mycorrhizal Fungi on Seed and Protein Yield under Water-Deficit Stress in MungBean. Agron. J.105:79–84.
  18. Heinemeyer, A., Fitter, A.H. 2004. Impact of temperature on the arbuscular mycorrhizal (AM) symbiosis: growth responses of the host plant and its AM fungal partner. J. Exp. Bot. 396:525–534.
  19. Kaya, C., Higgs, D., Kirnak, H., Tas, I. 2003. Mycorrhizal colonization improves fruit yield and water use efficiency in Watermelon (Citrullus lanatus Thunb.) grown under well-watered and water-stressed conditions. Plant and Soil 253(2): 287-292.
  20. Khalvati, M.A., Hu, Y., Mozafar, A., and Schmidhalter, U. 2005. Quantification of water uptake by arbuscular mycorrhizal hyphae and its significance for leaf growth, water relations, and gas exchange of barley subjected to drought stress. Plant Biol. 7:706–712.
  21. Kokubun, M., Shimada, S., and Takahashi, M. 2001. Flower abortion caused by pre-anthesis water deficit is not attributed to impairment of pollen in Soybean. Crop Sci. 41: 1517-1521.
  22. Lambert, D.H., and Weidensaul, T.C. 1991. Element uptake by mycorrhizal soybean from sewage-sludge-treated soil. Soil Sci. Soc. Am. J. 55:393–398.
  23. Liu, F., Andersen, M.N., and Jensen, C.R. 2003. Loss of pod set caused by drought stress is associated with water status and ABA content of reproductive structures in soybean. Func. Plant Bio. 30: 271-280.
  24. Mathur, N., and Vyas, A. 2000. Influence of arbuscular mycorrhizae on biomass production, nutrient uptake and physiological changes in Ziziphus mauritiana Lam. under water stress. J. Arid Environ. 45:191–195.
  25. Martin, P. 2008. Arbuscular mycorrhiza: the mother of plant root endosymbioses. Nat. Rev. Micro. 6:763–775.
  26. Pelletier, S., and Dionne, J. 2004. Inoculation rate of Arbuscular Mycorrhizal Fungi Glomus intraradices and Glomus etunicatum affects establishment of landscape Turf with no irrigation or fertilizer inputs.Crop Sci. 44: 335-338.
  27. Porcel, R., and Ruiz-Lozano, J.M. 2004. Arbuscular mycorhhizal influence on leaf water potential, solute accumulation and oxidative stress in soybean plants subjected to drought stress. J. Exp. Bot. 55:1743–1750.
  28. Robert, M. 2001. Water relations, drought and vesicular arbuscular mycorrhizal symbiosis. Springer-Verlag. Mycorrhiza 11: 3-42.
  29. Ruiz-lozano, J.M. and Azcon, R. 1995. Hyphal contribution to water uptake in mycorrhizal plants as affected by the fungal species and water status. Physiol. Plant 95: 472–478.
  30. Sanches-blanco, M.J., T. Ferrandez‚ T., Morales‚ M.A., Morte, A., and Alarcon, J.J. 2004. Variations in water status‚ gas exchange‚ and growth in Rosmarinus officinalis plant infected with Glomus deserticola under drought conditions. J. Plant Physio. 161: 675-682.
  31. Schussler, A., Schwarzott, D., and Walker, C. 2001. A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycol. Res. 105:1413–1421.
  32. Sylvia, D.M., Hammond, L.C., Bennett, J.M., Haas, J.H., and Linda, S.B. 1993. Field response of maize to a VAM fungus and water management. Agron. J. 85:193–198.
  33. Smith, S.E., and Read, D.J. 2008. Mycorrhizal symbiosis, 3rd edn. Academic, London.
  34. Wu, Q.S., and Xia, R.X. 2006. Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions. J. Plant Physiol. 163:417–425.
  35. Yooyongwech, S., Phaukinsang, N., Cha-Um, S., and Supaibulwatana, K. 2013. Arbuscular mycorrhiza improved growth performance in Macadamia tetraphylla L. grown under water deficit stress involves soluble sugar and proline accumulation. Plant Growth Regul. 69:285–293.

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