The Effects of Aerobic Exercise and Melatonin on Ca Activity in Rats

The Effects of Aerobic Exercise and Melatonin on Ca Activity in Rats

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Author(s): Erdinç SIKTAR, Elif SIKTAR, Yavuz AKKUS

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DOI: 10.18483/ijSci.717 403 966 52-56 Volume 4 - May 2015


The purpose of this study was to determine the effects of Aerobic exercise and Melatonin on carbonic anhydrase activity in rats. In this study, 24 healthy Spraque Dawley male rats, weighing 250–300 g, 4–6 months of age were used. The rats were divided into four equal groups. C; (Control Group), M; (Melatonin Group), E; (Exercise Group), ME, (Melatonin-Exercise Group). The exercise groups (n:12) were run for endurance training at 23oC. The exercise was caried out 6 days a week for 4 weeks.The intensity of endurance training was gradually increased during four weeks. A standard mild electric shock deterrent was used intermittently when necessary to coerce the rats to run. At the end of the fourth week, rats were killed by decapitation on each training day. According to the result of this study, the level of carbonic anhydrase decreases in control, melatonin and “exercise groups and melatonin decreases significantly in exercise group. As a result, it can be said that melatonin and exercise reduces the level of CA in mechanisms related to O2 consumption and CO2 production in organism by having an organizer role.


Melatonin, Carbonic anhydrase, Aerobic Exercise, Rat


  1. Frisch, H. Waldhauser, F., Waldhor, T., Müllner-Eidenbock, A., Schweitzer P. and K. (2004). Increase in 6-Hydroxymelatonin Excretion in Humans during Ascent to High Altitudes, The Journal of Clinical Endocrinology & Metabolism 89(9):4388–4390.
  2. Kaur C, Srinivasan KN, Singh J, Peng CM, Ling EA )2002( Plasma melatoninpinealocyte morphology, and surface receptors/antigen expression on macrophages macrophages/ microglia in the pineal gland following a high-altitude exposure. J Neurosci Res 67:533–543.
  3. Hardeland R, Madrid JA, Tandx et al., (2012). Melatonin, the circadian multioscillator system and health: the need for detailed analyses of peripheral melatonin signaling. J Pineal Res 2012; 52:139–166.
  4. Motilva V, Garcia-Maurino S, Taero E et al., (2011). New paradigms in chronic intestinal inflammation and colon cancer: role of melatonin. J Pineal Res 2011; 51:44–60.
  5. Kornatowskak, K., Szewczyk-Golec K, Czuczejko J et al. Effect of melatonin on the oxidative stress in erythrocytes of healthy young and elderly subjects. J Pineal Res 2007; 42:153–158.
  6. Nickkholgh A, Schneider H, Sobirey M et al., (2011). The use of high-dose melatonin in liver resection is safe: first clinical experience. J Pineal Res 2011; 50:381–388.
  7. Dun-Xian Tan, Lucien C. Manchester, Xiaoyan Liu, Sergio A. Rosales-Corral, Dario Acuna-Castroviejo and Russel J. Reiter (2013), Mitochondria and chloroplasts as the original sites of melatonin synthesis: a hypothesis related to melatonin’s primary function and evolution in eukaryotes. J. Pineal Res. 2013; 54:127–138.
  8. Gülçin I., Mehmet E. Büyukokuroglu, Münir Oktay and O. Irfan Kufrevioglu (2002). On the in vitro antioxidative properties of melatonin, J. Pineal Res. 2002; 33:167–171.
  9. Beydemir, S., Ciftci, M., Ozmen, I ., Büyükokuroglu, M.E., Ozdemir, H. and Küfrevioglu, O.I. (2000). Effects of some medical drugs on enzyme activities of carbonic anhydrase from human erythrocytes in vitro and from rat erythrocytes in vivo. Pharmacol. Res. 42, 187–191.
  10. Moroshima I, Matsui H, Mukawa H, hayashi K, Toki Y, Okumura K, et al (1998). Melatonin: A pineal hormone with antioxidant property protects against adriamycin cardiomyopathy in rats. Life Sci 1998;63:511-21.
  11. Kumar K.Vijay, M.U.R.Naidu (2002), Effect of oral melatonin on exercise-induced oxidant stress in healthy subjects. Indian Journal of Pharmacology 2002; 34: 256-259.
  12. Meydani M, Evans WJ(1993). Free radicals, exercise and aging, Yu BP, editor. Free Radical in Aging. Florida: CRC Press; 1993. p. 83-9.
  13. Bulbul, M., Hisar, O., Beydemir, S., Çiftci, M. and Kufrevioglu, O.I. (2003) J. The in vitro and in vivo inhibitory effects of some sulfonamide derivatives on rainbow trout (Oncorhynchus mykiss) erythrocyte carbonic anhydrase activity. J. Enz. Inhib. Med. Chem. 18, 371–375.
  14. Beydemir S. and Gulcin I., (2004) Effects of Melatonin on Carbonic Anhydrase from Human Erythrocytes In Vitro and from Rat Erythrocytes In Vivo. Journal of Enzyme Inhibition and Medicinal Chemistry, April 2004 Vol. 19 (2), pp. 193–197.
  15. Coban T.A., Beydemir , S., Gülçin, İ. and Ekinci, D. (2007), Morphine Inhibits Erythrocyte Carbonic Anhydrase in Vitro and in Vivo, Biol. Pharm. Bull. 30(12) 2257—2261.
  16. Gülçin I., Beydemir S.¸ Alici H. A., Elmastas¸ M., Büyükokuroglu M. E (2004), In vitro antioxidant properties of morphine. Pharmacol. Res., 49, 59—66.
  17. Beydemir S., Çiftçi M., Küfrevioglu O. I., Büyükokuroglu M. E., Effects of gentamicin sulfate on enzyme activities of carbonic anhydrase from human erythrocytes in vitro
  18. and from rat erythrocytes in vivo. Biol. Pharm. Bull., 25, 966—969 (2002).
  19. Rickli, E.E., Ghazanfar, SAS, Gibbons, B.H., Edsall, J.T., (1964). Carbonic anhydrases from human erythrocytes. Preparation and properties of two enzymes. J. Biol. Chem. 239: 1065-1078.
  20. Wilbur, K.M., Anderson, N.G., (1976). Electrometric and colorimetric determination of carbonic anhydrase. J. Biol. Chem. 176: 147-151.
  21. Hisar, O., Beydemir, S., Gülçin I., O .Irfan küfrevioglu & Claudiut, Süpüra (2005). Effects of low molecular weight plasma inhibitors of rainbow trout (Oncorhynchus mykiss) on human erythrocyte carbonic anhydrase-II isozyme activity in vitro and rat erythrocytes in vivo. Journal of Enzyme Inhibition and Medicinal Chemistry, February 2005; 20(1): 35–39.
  22. Gülçin I, Beydemir, Ş. And Hisar O (2005). The effect of _-tocopherol on the antioxidant enzymes activities and lipid peroxidation of rainbow trout (Oncorhynchus mykiss). Acta Veterinaria Hungarica, 53(4): 425-433.
  23. Şişecioğlu, M., Çankaya, M., Gülçin, I., Özdemir, M., (2009). The Inhibitory effect of propofol on actoperoxidase. Protein Peptide Lett. 16(1): 46-49.
  24. Beydemir, Ş., Gülçin I, Küfrevioglu ÖI, Çiftçi M (2003). Glucose 6-phosphate dehydrogenase: In vitro and In vivo effects of dantrolene sodium. Pol. J. Pharmacol. 55: 787-792.
  25. Kim, S.J., Reiter, R.J. and Qi, W (2000). Melatonin prevents oxidative damage to protein and lipid induced by ascorbate −Fe3+-EDTA: comparison with glutathione and α-tocopherol.Neuroendocrinol. Lett. 21, 269–276.
  26. Hochster, R.M., Kates, M. and Quastel, J.H(1972) Metabolic Inhibitors (Academic Press, New York), pp 71–89.
  27. Hochster, R.M., Kates, M. and Quastel, J.H(1973) Metabolic Inhibitors (Academic Press, New York), pp 66–82.
  28. Reiter, R.J., Tang, L., Garcia, J.J. and Munoz-Hoyos, A. (1997). Pharmacological actions of melatonin in oxygen radical pathophysiology. Life Sci. 60, 2255–2271.
  29. Supuran, C.T. and Scozzafava, A. (2002). Applications of Carbonic Anhydrase inhibitors and activators in therapy. Exp. Opin. Ther. Pat. 12, 217–242.
  30. Olcay H, Beydemir S., Ilhami Gulcin (2009). The Effects of Melatonin Hormone on Carbonic Anhydrase Enzyme Activity in Rainbow Trout(Oncorhynchus mykiss) Erythrocytes In Vitro and In Vivo. Turk J Vet Anim Sci, 29 (2005) 841-845.

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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