Effect of Stereotactic Hematoma Aspiration on the NSE Expression after Experimental Intracerebral Hemorrhage

Effect of Stereotactic Hematoma Aspiration on the NSE Expression after Experimental Intracerebral Hemorrhage

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Author(s): Yanan Wang, Zhaoxing Li, Chengjie Sun, Xiaoying Wang, Kai Luo, Hongyan Zhang, Ning Gu, Dehui Zhu

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DOI: 10.18483/ijSci.795 462 1043 58-67 Volume 4 - Aug 2015


Objective: To observe the effect of stereotactic hematoma aspiration on the expression of neuron-specific enolase (NSE) in perihematomal tissue after intracerebral hemorrhage. Methods: The model of intracerebral hemorrhage were established by using inracerebral stereotaxic injection of type IV collagenase method in male adult Wistar rats and were randomly divided into sham operation group, intracerebral hemorrhage group, and hemorrhage aspiration group. The neurological impairment in rats were evaluated by means of Bederson four grade. The morphology of nerve cells in cortex were observed by the hematoxylin-eosin staining. The expression of NSE in cortex was detected respectively by the immunohistochemistry and Western Blot. Results: The expression of NSE in the peripheral region of hematoma in hematoma aspiration group and intracerebral hemorrhge group was significantly higher than that in sham operation group each time point after intracerebral hemorrhage (P<0.05). The expression of NSE in the peripheral region of hematoma in hematoma aspiration group was slightly higher than that of intracerebral hemorrhage ground one day after intracerebral hemorrhage (P<0.05), but there was no significantly difference. Compared with intracerebra hemorrhage group, the expression of NSE in the peripheral region of hematoma in hemorrhage aspiration group was significantly decreased on 3rd and 5th day after intracerebral hemorrhage. Compared with hemorrhage aspiration group, the numbers of positive cells were significantly increased and the damage of the neurons in cortex was worsened than that in intracerebra hemorrhage group. Conclusion: The expression of NSE in perihematomal tissue after intracerebral hemorrhage was increased obviously after intracerebral hemorrhage. However, early hematoma aspiration could weaken the expression of NSE, reduce the number of neuronal degeneration cells, and improved neurobehavioral function significantly after intracerebral hemorrhage.


NSE, Intracerebral Hemorrhage, Stereotactic Hematoma Aspiration, Rat


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