Author(s): Luo Xu, Heling Hao, Feifei Guo, Xiangrong Sun, Yanling Gong
The orexin neuropeptide family consists of orexin-A and orexin-B, which are coded from the same prepro-mRNA. Although peripherally administered orexin-A abolishes small intestinal interdigestive contractions in rats, it still remains unclear whether orexin-A of lateral hypothalamic area(LHA) effects on the gastric motility in rats. We selected Wistar rats as our subjects. A stainless-steel injection cannula was implanted unilaterally into the LHA. A force transducer was embedmented into the stomach to record circular muscle contractiontions in freely moving conscious rats. The gastric motility was monitored by administration of ghrelin into LHA. The changes of amplitude of constriction and frequency of gastric motility were monitored in conscious rats by a transducer. Subdiaphragmatic vagotomy was performed to elucidate the neural pathways of orexin-A. After microinjection of orexin-A into LHA 5-20 min later, Orexin-A dose-dependently increased the amplitude of contraction and accelerated frequency of gastric motility in the stomach. The effect of orexin-A on promoting the amplitude and frequency of gastric contraction disappeared after 0.5μg orexin-A +6.0μg SB-334867 mixture was microinjection into LHA. However, there was no significant changes of amplitude and frequency of gastric contraction while injection of orexin-A receptor antagonists SB-334867 alone. The stimulatory effect of orexin-A on gastric motility was abolished by subdiaphragmatic vagotomy. It is suggested that exogenous orexin-A of LHA may promote gastric motility, the effect may be accomplished through the LHA-vagus pathway.
orexin-A, lateral hypothalamic area(LHA), gastric motility, SB-334867
- De Lecea L, Kilduff TS, Peron C, et al. The hypocretins: hypothalamus-specific peptides with neuroexcitatory activity. Proc Natrl Acad Sci, 1998;95(1):322–327.
- Sakurai T, Amemiya A, Ishii M, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding bahaviour. Cell, 1998;92(4):573–585.
- T.A. Butterick, et al., Orexin: pathways to obesity resistance? Rev. Endocr. Metab. Disord. 2013;14 (4):357–364.
- Liblau RS, Vassalli A, Seifinejad A, Tafti M. Hypocretin (orexin) biology and the pathophysiology of narcolepsy with cataplexy. Lancet Neurol, 2015;14:318–328. The hypocretins: hypothalamus-specific peptides with neuroexcitatory activity.
- Brown JA, Woodworth HL, Leinninger GM. To ingest or rest? Specialized roles of lateral hypothalamic area neurons in coordinating energy balance. Front Syst Neurosci. 2015; 18:9-18.
- Fernø J, Señarís R, Diéguez C, Tena-Sempere M, López M. Orexins (hypocretins) and energy balance: More than feeding. Mol Cell Endocrinol.2015;26. pii: S0303-7207(15)30029-0.
- Palus K, Chrobok L, Lewandowski MH. Orexins/hypocretins modulate the activity of NPY-positive and -negative neurons in the rat intergeniculate leaflet via OX1 and OX2 receptors. Neuroscience. 2015;6:370-380.
- Kirchgessner, AL. Orexins in the brain–gut axis. Endocr. Rev. 2002;23:1–15.
- Bülbül M, Babygirija R, Zheng J, Ludwig KA, Takahashi T. Central orexin-A changes the gastrointestinal motor pattern from interdigestive to postprandial in rats. Auton Neurosci. 2010;158(1-2):24-30.
- Paranjape S, Vavaiya K, Kale A, Briski K. Role of dorsal vagal motor nucleus orexin-receptor-1 in glycemic responses to acute versus repeated insulin administration. Neuropeptides , 2007;41:111–116.
- Krowicki ZK, Burmeister MA, Berthoud HR, et al. Orexins in rat dorsal motor nucleus of the vagus potently stimulate gastric motor function. Am. J. Physiol. Gastrointest. Liver Physiol. 2002;283:G465–G472.
- Kobashi M, Furudono Y, Matsuo R, Yamamoto T. Central orexin facilitates gastric relaxation and contractility in rats. Neurosci. Lett. 2002;332:171–174.
- Paxinos G., Watson C. The Rat Brain in Stereotaxic Coordinates, Academic Press Inc., San Diego, CA, 2007.
- Li ZL, Xu L, Sun XR, et al. Central nesfatin-1 influences the excitability of ghrelin-responsive gastric distension neurons in the arcuate nucleus and reduces gastric motility in rats. Eur J Neurosci. 2013;38(11):3636-3643.
- Taniguchi H, Ariga H, Zheng J, et al. Endogenous ghrelin and 5-HT regulate interdigestive gastrointestinal contractions in conscious rats. Am. J. Physiol. Gastrointest. Liver Physiol. 2008;295:G403–G411
- Wang Q, Guo F, Sun X, Gao S, Li Z, Gong Y, Xu L. Effects of exogenous nesfatin-1 on gastric distention-sensitive neurons in the central nucleus of the amygdala and gastric motility in rats. Neurosci Lett. 2014;17,582:65-70.
- Xu L, Gong Y, Wang H, et al. The stimulating effect of ghrelin on gastric motility and firing activity of gastric-distension-sensitive hippocampal neurons and its underlying regulation by the hypothalamus. Exp Physiol. 2014;99(1):123-35.
- Guo FF, Xu L, Gao SL, et al. The effects of nesfatin-1 in the paraventricular nucleus on gastric motility and its potential regulation by the lateral hypothalamic area in rats. J Neurochem. 2015;132(3):266-275.
- Shioda S, Takenoya F, Yagi M, et al. Neural networks of several novel neuropeptides involved in feeding regulation. Nutrition, 2008;24:48–853.
- Gong Y, Xu L, Wang H, Guo F, Sun X, Gao S. Involvements of the lateral hypothalamic area in gastric motility and its regulation by the lateral septum. Gen Comp Endocrinol. 2013;194:275-85.
- Wang Y, Chen F, Shi H, et al. Extrinsic ghrelin in the paraventricular nucleus increases small intestinal motility in rats by activating central growth hormone secretagogue and enteric cholinergic receptors.Peptides. 2015;74:43–49.
- Okumura T, Nozu T. Role of brain orexin in the pathophysiology of functional gastrointestinal disorders. J Gastroenterol Hepatol. 2011;26:61-67.
- Xu L, Gao S, Guo F, et al. Effect of motilin on gastric distension sensitive neurons in arcuate nucleus and gastric motility in rat.Neurogastro. Motil. 2011;23(3):121–265.
Cite this Article:
International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.