The Ghrelin/GHS-R1a Pathway is Involved in the Mechanism of the CTA-regulated Neuronal Loop

The Ghrelin/GHS-R1a Pathway is Involved in the Mechanism of the CTA-regulated Neuronal Loop

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Author(s)

Author(s): Qian Wang

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DOI: 10.18483/ijSci.1182 273 631 64-72 Volume 6 - Jan 2017

Abstract

Objective: We aimed to investigate the effects of ghrelin and its receptor GHS-R1a on the regulation of taste aversion-associated emotional memory. Methods: We studied the effect of selective and reversible inactivation of emotion-associated neurons on emotions processed by the local neuronal loop of the lateral amygdala and insular cortex. We used intraperitoneal clozapine-N oxide (CNO) injection, local brain microinjection, and taste aversion test. Results: Microinjection of ghrelin into the lateral amygdala blocked the acquisition of taste aversion memory in mice. However, microinjection of ghrelin did not affect memory consolidation. Adeno-associated virus (AAV) microinjection into the lateral amygdaloid nucleus blocked the acquisition of taste aversion memory in mice. Moreover, intraperitoneal injection of CNO inhibited taste aversion memory formation, altering the aversion index. Conclusion: Microinjection of ghrelin and AAV-CaMKII-hM4Di-2A-GHS-R1a-GFP into the lateral amygdala inhibited the acquisition of conditioned taste aversion in mice. The inhibition of the memory formation was achieved through the activation of growth hormone secretagogue receptor 1a (GHS-R1a).

Keywords

Ghrelin, insular cortex, CNO, CTA, GI-DREADD System

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Cite this Article:

International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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