Effect of ATF5 in Mice Transfected with Human Cytomegalovirus IE2 Gene

Effect of ATF5 in Mice Transfected with Human Cytomegalovirus IE2 Gene

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Author(s)

Miao Yu, Ting Liu, Ming Hu, Bin Wang, Dongmeng Qian

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DOI: 10.18483/ijSci.1829 Downloads: 22 Views: 85 Pages: 35-38 Volume 7 - Nov 2018

Abstract

To investigate the qualitative and quantitative detection of ATF5 activated transcription factors in tumor tissue by using the method of transfecting IE2 mouse tumor to explore the effect of HCMV immediate early protein IE86 on ATF5 function. Transfected mice were treated with GL261 mouse glioma cells. The expression of transcription factor ATF5 in the tumor tissues of tumor-bearing mice was detected by PCR and Western-blotting methods. The experimental results showed that the expression of ATF5 was higher in IE2 transgenic mice than in normal mice. ATF5 is highly expressed in IE2 transgenic mice. ATF5 is a transcription factor closely related to apoptosis and plays an important role in apoptosis, differentiation and development.

Keywords

Human Cytomegalovirus, IE86, Mouse Glioma Cell, ATF5

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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Volume 7, November 2018


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