Research Progress on Exosomes Derived from Human Adipose Mesenchymal Stem Cells

Research Progress on Exosomes Derived from Human Adipose Mesenchymal Stem Cells

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Author(s): Wang Jing, Wang Zhiguo, Cai Xia, Li kun, Hao Rongan, An Yu

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DOI: 10.18483/ijSci.1984 12 90 114-117 Volume 8 - Mar 2019


Mesenchymal stem cells (MSC) have the potential of multi-directional differentiation, have stable genetic information, can self-replicate and renew, and are commonly used as seed cells in the field of regeneration1. Adipose mesenchymal stem cells, bone marrow mesenchymal stem cells and umbilical cord mesenchymal stem cells are the most commonly used mesenchymal stem cells. Compared with other mesenchymal stem cells, adipose mesenchymal stem cells have a wide range of sources, relatively simple acquisition methods and less trauma2. Therefore, adipose mesenchymal stem cells have gradually become a new favorite in stem cell research. However, due to the harsh storage conditions and inconvenient transportation of living cells, the survival rate of direct transplantation into living organisms is low, which makes it difficult for clinical application. Exosome is a kind of extracellular secretory vesicle, which exists in almost all body fluids and can be extracted from the supernatant of most cultured cells in vitro3. It has the characteristics of secretory cells, simple storage, convenient transportation and low immunogenicity, providing a new direction for the clinical application of adipose mesenchymal stem cells. Therefore, this paper reviews the exosomes derived from adipose mesenchymal stem cells.


Adipose-Derived Stem Cells, ADSCs, Mesenchyma Stem Cell, Exosomes


  1. Liao, H. T. & Chen, C. T., Osteogenic potential: Comparison between bone marrow and adipose-derived mesenchymal stem cells. WORLD J STEM CELLS 6 288 (2014).
  2. Daniel A, D. U., Peter H, A., Amir, E. & Marc H, H., Future of fat as raw material for tissue regeneration. ANN PLAS SURG 50 (2003).
  3. Raposo, G. & Stoorvogel, W., Extracellular vesicles: Exosomes, microvesicles, and friends. The Journal of Cell Biology 200 373 (2013).
  4. Zuk, P. A. et al., Multilineage cells from human adipose tissue: implications for cell-based therapies. TISSUE ENG 7 (2001).
  5. Patricia A. Zuk, M. Z. P. A. & Prosper Benhaim, A. M. H. H., Human Adipose Tissue Is a Source of Multipotent Stem Cells. MOL BIOL CELL (2002).
  6. Cardozo, A. J., Gomez, D. E. & Argibay, P. F., Transcriptional characterization of Wnt and Notch signaling pathways in neuronal differentiation of human adipose tissue-derived stem cells. J MOL NEUROSCI 44 186 (2011).
  7. E, V. D. P. et al., Optical and non-optical methods for detection and characterization of microparticles and exosomes. J THROMB HAEMOST 8 2596 (2010).
  8. Navid Mohamadpour, T., Marlene Louise, C., S Ren Paludan, S. & Jens Ahm, S. R., Adipose-Derived Stem Cells: New Treatment for Wound Healing? ANN PLAS SURG 75 (2015).
  9. John K, F., Isabella, W., Zeni, A. & Marc H, H., Fat tissue: an underappreciated source of stem cells for biotechnology. TRENDS BIOTECHNOL 24 (2006).
  10. Yoshimura, K. et al., Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J CELL PHYSIOL 208 64 (2006).
  11. Kevin McIntosh, S. Z. S. G., The Immunogenicity of Human Adipose-Derived Cells: Temporal Changes In Vitro. STEM CELLS 24 (2006).
  12. Aust, L. et al., Yield of human adipose-derived adult stem cells from liposuction aspirates. CYTOTHERAPY 6 7 (2004).
  13. Li-xin, L. et al., Comparison of biological characteristics of adipose-derived stem cells from different parts. Journal of Clinical Rehabilitative Tissue Engineering Research 4992 (2013).
  14. Stosich, M. S. & Mao, J. J., Adipose tissue engineering from human adult stem cells: clinical implications in plastic and reconstructive surgery. PLAST RECONSTR SURG 119 71, 84 (2007).
  15. Huang, J. I. et al., Rat extramedullary adipose tissue as a source of osteochondrogenic progenitor cells. PLAST RECONSTR SURG 109 1033, 1042 (2002).
  16. Jaiswal, N., Haynesworth, S. E., Caplan, A. I. & Bruder, S. P., Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J CELL BIOCHEM 64 295 (1997).
  17. D M, S., G N, K. & D V, C., Collagen synthesis in cultured osteoblast-like cells. ARCH BIOCHEM BIOPHYS 201 (1980).
  18. Backesjo, C. M., Li, Y., Lindgren, U. & Haldosen, L. A., Activation of Sirt1 decreases adipocyte formation during osteoblast differentiation of mesenchymal stem cells. CELLS TISSUES ORGANS 189 93 (2009).
  19. Jm, G. & F, G., Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. CYTOTHERAPY 5 (2003).
  20. Ogawa, R. et al., Osteogenic and chondrogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice. Biochem Biophys Res Commun 313 871 (2004).
  21. Winter, A. et al., Cartilage-like gene expression in differentiated human stem cell spheroids: a comparison of bone marrow-derived and adipose tissue-derived stromal cells. Arthritis Rheum 48 418 (2003).
  22. Martinez-Conesa, E. M., Espel, E., Reina, M. & Casaroli-Marano, R. P., Characterization of ocular surface epithelial and progenitor cell markers in human adipose stromal cells derived from lipoaspirates. Invest Ophthalmol Vis Sci 53 513 (2012).
  23. Zhu, Y. et al., Adipose-derived stem cell: a better stem cell than BMSC. CELL BIOCHEM FUNCT 26 664 (2008).
  24. Jianhui, Z., Development in basic research of adipose-derived stromal/stem cells. (2011).
  25. van der Pol, E., Boing, A. N., Harrison, P., Sturk, A. & Nieuwland, R., Classification, Functions, and Clinical Relevance of Extracellular Vesicles. PHARMACOL REV 64 676 (2012).
  26. Wolf, P., The nature and significance of platelet products in human plasma. Br J Haematol 13 269 (1967).
  27. Dalton, A. J., Microvesicles and vesicles of multivesicular bodies versus "virus-like" particles. J Natl Cancer Inst 54 1137 (1975).
  28. Johnstone, R. M., Adam, M., Hammond, J. R., Orr, L. & Turbide, C., Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes). J BIOL CHEM 262 9412 (1987).
  29. Lundy, S. K., Klinker, M. W. & Fox, D. A., Killer B lymphocytes and their fas ligand positive exosomes as inducers of immune tolerance. FRONT IMMUNOL 6 122 (2015).
  30. Lu, J., Wu, J., Tian, J. & Wang, S., Role of T cell-derived exosomes in immunoregulation. IMMUNOL RES 66 313 (2018).
  31. Chen, X. et al., Exosomes derived from hypoxic epithelial ovarian cancer deliver microRNA-940 to induce macrophage M2 polarization. ONCOL REP 38 522 (2017).
  32. Tan, J. L. et al., Amnion Epithelial Cell-Derived Exosomes Restrict Lung Injury and Enhance Endogenous Lung Repair. Stem Cells Transl Med 7 180 (2018).
  33. Ju, R. et al., Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) and syndecan-4/syntenin pathways. J BIOL CHEM 289 510 (2014).
  34. Mead, B. & Tomarev, S., Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms. Stem Cells Transl Med 6 1273 (2017).
  35. Picciolini, S. et al., Detection and Characterization of Different Brain-Derived Subpopulations of Plasma Exosomes by Surface Plasmon Resonance Imaging. ANAL CHEM 90 8873 (2018).
  36. Street, J. M., Koritzinsky, E. H., Glispie, D. M. & Yuen, P. S. T., Urine Exosome Isolation and Characterization. Methods Mol Biol 413 (2017).
  37. Hong, C. S., Funk, S., Muller, L., Boyiadzis, M. & Whiteside, T. L., Isolation of biologically active and morphologically intact exosomes from plasma of patients with cancer. J Extracell Vesicles 5 29289 (2016).
  38. Hock, A. et al., Breast milk-derived exosomes promote intestinal epithelial cell growth. J PEDIATR SURG 52 755 (2017).
  39. Machida, T. et al., miR1246 and miR4644 in salivary exosome as potential biomarkers for pancreatobiliary tract cancer. ONCOL REP 36 2375 (2016).
  40. Menay, F. et al., Exosomes Isolated from Ascites of T-Cell Lymphoma-Bearing Mice Expressing Surface CD24 and HSP-90 Induce a Tumor-Specific Immune Response. FRONT IMMUNOL 8 286 (2017).
  41. Gruenberg, J. & van der Goot, F. G., Mechanisms of pathogen entry through the endosomal compartments. Nat Rev Mol Cell Biol 7 495 (2006).
  42. Gould, S. J. & Raposo, G., As we wait: coping with an imperfect nomenclature for extracellular vesicles. Journal of Extracellular Vesicles 2 (2013).
  43. Trajkovic, K. et al., Ceramide triggers budding of exosome vesicles into multivesicular endosomes. SCIENCE 319 1244 (2008).
  44. Thery, C., Zitvogel, L. & Amigorena, S., Exosomes: composition, biogenesis and function. NAT REV IMMUNOL 2 569 (2002).
  45. Thery, C. et al., Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73. J CELL BIOL 147 599 (1999).
  46. Dorayappan, K., Wallbillich, J. J., Cohn, D. E. & Selvendiran, K., The biological significance and clinical applications of exosomes in ovarian cancer. GYNECOL ONCOL 142 199 (2016).
  47. Tkach, M. & Thery, C., Communication by Extracellular Vesicles: Where We Are and Where We Need to Go. CELL 164 1226 (2016).
  48. Yoon, Y. J., Kim, O. Y. & Gho, Y. S., Extracellular vesicles as emerging intercellular communicasomes. BMB REP 47 531 (2014).
  49. Ailawadi, S., Wang, X., Gu, H. & Fan, G. C., Pathologic function and therapeutic potential of exosomes in cardiovascular disease. Biochim Biophys Acta 1852 1 (2015).
  50. Huang, L. et al., Exosomes in mesenchymal stem cells, a new therapeutic strategy for cardiovascular diseases? INT J BIOL SCI 11 238 (2015).
  51. A Aryani, B. D., Exosomes as a Nanodelivery System: a Key to the Future of Neuromedicine? MOL NEUROBIOL 2 (2014).
  52. Lopez-Verrilli, M. A. et al., Mesenchymal stem cell-derived exosomes from different sources selectively promote neuritic outgrowth. NEUROSCIENCE 320 129 (2016).
  53. Baglio, S. R. et al., Human bone marrow- and adipose-mesenchymal stem cells secrete exosomes enriched in distinctive miRNA and tRNA species. STEM CELL RES THER 6 127 (2015).

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International Journal of Sciences is Open Access Journal.
This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Author(s) retain the copyrights of this article, though, publication rights are with Alkhaer Publications.

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