Heterosis and Heritability of Chemical Quality of Butter Derived from Some Cocoa (Theobroma Cacao L.) Clones

Heterosis and Heritability of Chemical Quality of Butter Derived from Some Cocoa (Theobroma Cacao L.) Clones

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Author(s): Pierre Effa Onomo, Mbia Ronelle Fabiola, Achu Mercy Bih, Martine Louise Ondobo, Kancsi Germain

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DOI: 10.18483/ijSci.1043 393 749 57-64 Volume 5 - Jun 2016


Butter from dry cocoa beans of five cocoa (Theobroma cacao L.) clones and their intercrossed hybrids were analyzed for chemical quality (acid, peroxide and iodine) in order to gain insight on the heterosis and broad-sense heritability values for these traits. Standard procedures were used for estimation of the lipid content and the indices. Hybrid F20 had the highest lipid content (59.7%) while the parent SCA12 and hybrid F12 had the lowest with 44.5 and 45% respectively. The acid index varied between 19.4 and 28.8 mg KOH/g, iodine index from 10.2 to 30.5 g/100g and peroxide index from 0.3 to 7.3 meq.g O2/kg. The acid index was higher than recommended limits for edible oils, iodine index lower and peroxide index within the international norms for conventional oils. Hybrids F12, F40 and F79 presented a positive mid-parent heterosis for acid index while F14, F13, F20 and F79 showed it for peroxide index. With regards to best-parent heterosis, only F40, F13 and F79 for acid index and F14 and F13 for peroxide index showed positive heterosis effect. For iodine index, about 71 % of hybrids showed a positive mid-parent heterosis. The acid and peroxide indices showed high heritability (while the iodine index had moderate heritability) estimates and this heritability could be said to be cytoplasmic except for the reciprocal hybrids F13 and F79 (acid index). The Principal component analysis categorized two distinct groups of six individuals each. Quality characteristics were moderate for butter obtained from the first cluster and lower for the second one. When all these quality parameters are taken into account, F79 and its reciprocal hybrid F13 showed the highest hybrid vigour. Their parents can be therefore considered as the best combination for producing hybrids with high nutritional oil quality vigour.


Cocoa butter, parameters of qualities, heterosis, heritability, Theobroma cacao L.


  1. Andres-Lacueva C., Monagas M., Khan N, Izquierdo-Pulido M., Urpi-Sardam M., Permanyerm J., Lamuela-Raventos & R.M. (2008). Flavanol and Flavonol Contents of Cocoa Powder Products: Influence of the Manufacturing Process. J. Agric. Food Chem. (56): 3111-3117. DOI:10.1021/jf0728754
  2. AFNOR - Association Française de Normalisation 1981
  3. Ardhana M.M. & Fleet G.H. (2003).The microbial ecology of cocoa bean fermentation in Indonesia. International Journal of Food Microbiology 86: 87-99. DOI:10.1016/S0168-1605(03)00081-3
  4. Atasie V. N., Akinhanmi T. F. & Ojiodu C. C. (2009). Proximate Analysis and Physico-Chemical Properties of Groundnut (Arachis hypogaea L.). Pakistan Journal of Nutrition 8 (2): 194-197. DOI: 10.3923/pjn.2009.194.197
  5. Beckett S.T. 1994. Industrial Chocolate Manufacture and Use. 2nd Ed., Blacki academic and professional, Glasgow, United Kingdom.
  6. Cilas C. (1991). Estimation of some genetic parameters of different crosses plants of cocoa. Café Cacao Thé 25:3-13.
  7. Codex Alimentarius Commission (1992). Programme mixte FAO/OMS sur les normes alimentaires. FAO, Rome.
  8. Dhellot J.R., Matouba E., Maloumbi M.G., Nzikou J.M., Safou-Ngoma D.G., Linda M., Desobry S. & Parmentier M. (2006). Extraction, chemical composition and nutritional characterization of vegetable oils: case of Amaranthus hybridus (Var1 and 2) of Congo Brazzaville. Afr. J. Biotechnol. 5(11):1095-1101.
  9. Effa O. P., Niemenak N., Djocgoue P. F., Ondobo M. L. & Omokolo N. D. (2015). Heritability of polyphenols, anthocyanins and antioxidant capacity of Cameroonian cocoa (Theobroma cacao L.) beans. Afr. J. Biotechnol. 14(36): 3672-2682. DOI: 10.5897/AJB2015.14715
  10. Fokou E., Achu M. B. & Tchounguep F. M. (2004). Preliminary nutritional evaluation of five species of egusi seeds in Cameroon. African Journal of Food, Agriculture, Nutrition and Development 4: 1-11. DOI.org/10.4314/ajfand.v4i1.19151
  11. Fokou E., Achu M.B., Kansci G., Ponka R., Fotso M., Tchiégan C. & Tchouanguep F.M. (2009). Chemical Properties of Some Cucurbitaceae Oils from Cameroon. Pakistan Journal of Nutrition 8(9): 1325-1334. DOI: 10.3923/pjn.2009.1325.1334
  12. Folch J., Lees M. & Sloane S.G.H. (1957). A simple method for the isolation of total lipids from animal tissues. Journal of Biological Chemistry 226:497-509
  13. Hui L. (1996). Edible oil and fat products: oils and oilseeds, bailey’s industrial oil and fat products, 5th Ed. Wiley, New York. pp.109 - 110. DOI: 10.1002/047167849X.bio066
  14. Idah P.A., Meshack S.I. & Mohammed M.A. (2014). Extraction and Characterization of Cashew Nut (Anacardium Occidentale) Oil and Cashew Shell Liquid Oil. Academic Research International, 5(3): 50-54. DOI : 10.1007/BF02885447
  15. Kanematsu H., Maruyama T., Niiya I., Imamura M. & Matsumoto T. (1978b). Studies on the hard butter. I. On the components of cocoa butter. Journal of the Japanese Oil Chemist’s Society 27: 385-389. http://doi.org/10.5650/jos1956.27.385
  16. Klagge P. & SenGupta A.K. (1990) Kakaobutter und ihre Alternativen fir Schokoladenprodukte. European Journal of Food Science and Technology 92(12): 485-492. DOI: 10.1002/lipi.19900921208
  17. Kiritsakis A.K. & Min D.B. (1989). Flavor chemistry of olive oil in: Flavor chemistry of lipids foods Chap. 11. Eds Min DB., Ohio state university, Colombus, Ohio and Smouse TH, Archer Daniels Midland, Decatur Illinois 62525.
  18. Krishnamurthy R.G. (1982). Cooking oils, salad oils and salad dressings. In Bailey’s Industrial Oils and Fat Products (Vol. 2, 4th ed.), Swern D (eds.). John Wiley and Sons Inc.: New-York (USA); 603 p.
  19. Lipp M. & Anklam E. (1998). Review of cocoa butter and alternative fats for use in chocolate-Part A. Compositional data. Food Chemistry 62 (1): 73-97. DOI:10.1016/S0308-8146(97)00160-X
  20. Lynch M. & Walsh B. (1998). Genetics and Analysis of Quantitative Traits. Sinauer Associates, Sunderland, MA.
  21. Mbondji M.P. (2010). Cocoa tree in Cameroon. The Catholic University of Central Africa Edition, pp 254.
  22. Phudenpa A., Joglo, S., Toomsan B., Wongkaew S., Kesmala T. & Patanothai A. (2004). Heritability and phenotypic correlation of traits related to N2-fixation and agronomic traits in peanut (Arachis hypogaea L.). Songklanakarin Journal of Science Technology 26 (3): 317-325.
  23. Tang F. & Xiao W. (2013). Genetic effects and heterosis of within-boll yield components in upland cotton (Gossypium hirsutum L.). Euphytica 194: 41-51. DOI :10.1007/s10681-013-0958-3
  24. Xanthopoulou M.N., Nomikos T., Fragopoulou E. & Antonopoulou S. (2009). Antioxidant and lipoxygenase inhibitory activities of pumpkin seed extracts. Food Research International, 42(5-6): 641-646. DOI:10.1016/j.foodres.2009.02.003

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International Journal of Sciences is Open Access Journal.
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